RNAMAPPER.ORG
RNAmapperRNAmapper is a bioinformatic pipeline described in Miller et al. (accepted Genome Research). It enables the use of RNA-Seq data to map and identify mutations in zebrafish.
http://www.rnamapper.org/
RNAmapper is a bioinformatic pipeline described in Miller et al. (accepted Genome Research). It enables the use of RNA-Seq data to map and identify mutations in zebrafish.
http://www.rnamapper.org/
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RNAmapper | rnamapper.org Reviews
https://rnamapper.org
RNAmapper is a bioinformatic pipeline described in Miller et al. (accepted Genome Research). It enables the use of RNA-Seq data to map and identify mutations in zebrafish.
rnamapper.org
Galaxy Online - RNAmapper
http://www.rnamapper.org/Online-Setup
Methods for performing the pipeline using Galaxy on the Amazon Cloud. Here you will find information on the process of setting up your own Galaxy server with RNAmapper. In the cloud using Amazon Web Services and the Amazon Elastic Compute Cloud. We have created an Amazon Machine Image that contains the same components as the downloadable version, but you can use Amazon's computing power to analyze your data. And there is specific help for implementing an Amazon version ( Galaxy Amazon help. Under Communi...
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Galaxy 101 - RNAmapper
http://www.rnamapper.org/rnamapper-101
Methods for using RNAmapper on Galaxy. This tutorial briefly explains how to use RNAmapper. In Galaxy. Galaxy is a vast toolbox for data analysis, going far beyond what is presented here. However, you have the option to use RNAmapper in a turnkey way by using the provided workflows as described below. Most of this tutorial is concerned with uploading data. Once your data is uploaded, all you need to do is click on a button and come back 24 48 hrs. USING YOUR RNAMAPPER GALAXY PROGRAM. First open Firefox, ...
In Vivo Methods - RNAmapper
http://www.rnamapper.org/in-vivo-methods
Experimental set up for mapping mutations using RNA-seq data. Below are the methods we used to maintain fish, set up crosses and pool embryos, extract RNA from those pools, create cDNA and Illumina libraries, and perform RNA-Se q. CDNA and Illumina library preparation. We used the Illumina TruSeq kit. Preparation method to create sequencing libraries from our RNA samples.
RNAmapper
http://www.rnamapper.org/home
This is the home of the bioinformatics pipeline RNAmapper - Miller, Obholzer, Shah, Megason, and Moens (Genome Research, 2013). Pubmed - http:/ www.ncbi.nlm.nih.gov/pubmed/23299976. You will need to prepare your samples and perform the RNA-seq experiment - in vivo methods. You will need to use the RNA-seq data to map your mutations. We provide two RNAmapper implementations:. From the command line - command line 101. RNAmapper using Galaxy - galaxy download. Now go clone your genes! The black marks repres...
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Resources - millerlab
http://millerlab.brainbuild.org/software
One of the coolest emerging genetic technologies is CRISPR. Which allows for unprecedented control over modifying genomes. We took advantage of CRISPR's high rate of mutagenesis to perform the first in vivo. Reverse genetic screen in a vertebrate. We screened for electrical synapse defects but also found that the approach was applicable to many different developmental processes. The method will greatly facilitate the interrogation of gene function in vertebrates.
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RNAmapper
This is the home of the bioinformatics pipeline RNAmapper - Miller, Obholzer, Shah, Megason, and Moens (Genome Research, 2013). Pubmed - http:/ www.ncbi.nlm.nih.gov/pubmed/23299976. You will need to prepare your samples and perform the RNA-seq experiment - in vivo methods. You will need to use the RNA-seq data to map your mutations. We provide two RNAmapper implementations:. From the command line - command line 101. RNAmapper using Galaxy - galaxy download. Now go clone your genes! The black marks repres...
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RNA Matters | Musings on the important molecule known as RNA
Musings on the important molecule known as RNA. Skip to primary content. Skip to secondary content. Heteroplasmy and exchange of mitochondrial DNA. October 21, 2014. A very interesting paper appeared on the BiorXiv server. It is sort of a hybrid paper: it presents a new method (Mseek) for enriching and isolating mitochondrial (MT) DNA as well as it reports results that demonstrate the transfer of MT DNA between cell lines. Sort of like ‘horizontal gene transfer’ for eukaryotes. How many tRNAs are there?
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Software Developer, Game Nerd, Hip Hop Head and more. My name is Rohit Nambiar and i currently live in San Francisco CA. This is just a site for me to place projects i'm working on and to link my blog to this site, which i am trying to update as much as possible. Enjoy! Click Here to Download. Few recent pictures from my Instagram. Created with BlueprintCSS, jQuery, FancyBox and TextMate.
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