adrian-protein.blogspot.com
Dimension: Insoluble protein?!
http://adrian-protein.blogspot.com/2010/08/insoluble-protein.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Wednesday, August 18, 2010. According to SDS P.A.G.E for solubility which I did on 18 AUG, the result doesn't looks good. Maybe it is better to subclone the stored plasmid to Rosetta-gami2 (DE3) and BL21 (DE3) , BL21 (DE3)/pLySs? At the same time, design primer and clone into pCold and pMAL.
adrian-protein.blogspot.com
Dimension: August
http://adrian-protein.blogspot.com/2010/08/august.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Monday, August 16, 2010. Last 2 weeks screening for the culture of RG2 pET32 phaCcs between 2 culture from glycerol stock. As a result, one of the bacteria was contamination, didn't express protein phaC and didnt contain the correct plasmids. 17 AUG 2010 (Done). 1 Purification by TALON.
adrian-protein.blogspot.com
Dimension: The definition of biodegradability
http://adrian-protein.blogspot.com/2010/12/definition-of-biodegradability.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Wednesday, December 22, 2010. The definition of biodegradability. Towards Common Ground –. Meeting Summary of the International Workshop on Biodegradability,. Annapolis, MD, USA, 1992. Such as composting, sewage treatment, denitrification, or anaerobic sludge treatment. Stuck @ dec 2010.
adrian-protein.blogspot.com
Dimension: Crystallization tutorial
http://adrian-protein.blogspot.com/2014/06/crystallization-tutorial.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Wednesday, June 4, 2014. This is a very simple tutorial about protein crystallization:. Http:/ xray.bmc.uu.se/ terese/tutorials.html. Bergfors, T., Editor. Protein Crystallization: Second Edition. 2009. International. University Line, La Jolla, California, 500 pp. KAS project by hani hadi.
adrian-protein.blogspot.com
Dimension: December 2010
http://adrian-protein.blogspot.com/2010_12_01_archive.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Wednesday, December 22, 2010. The definition of biodegradability. Towards Common Ground –. Meeting Summary of the International Workshop on Biodegradability,. Annapolis, MD, USA, 1992. Such as composting, sewage treatment, denitrification, or anaerobic sludge treatment. Stuck @ dec 2010.
adrian-protein.blogspot.com
Dimension: August 2010
http://adrian-protein.blogspot.com/2010_08_01_archive.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Sunday, August 22, 2010. This week will be a busy week starting from tomorrow. Maybe should say it is a busy month too. I do not want to waste my time anymore. So let's get to work. An amount of bacterial hosts and expression vectors appearing in my minds now. The NEB express - ER2523. As a ...
adrian-protein.blogspot.com
Dimension: Stuck @ dec 2010
http://adrian-protein.blogspot.com/2010/12/stuck-dec-2010.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Wednesday, December 8, 2010. Stuck @ dec 2010. E coli Rosettagami2(DE3) with pET32 harbouring pha. Problem 1: Expression level of PhaC is very low compare to previous batches. Less than 10% , will be very difficult to be purified). Problem 2: Almost 100% of the expressed PhaC is insoluble.
adrian-protein.blogspot.com
Dimension: July 2010
http://adrian-protein.blogspot.com/2010_07_01_archive.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Friday, July 30, 2010. Was trying to produce more protein from the 1.6L induced bacterial cultures. Purified with Affinity chromatography successfully. (Although lost an amount of protein due to my mistakes). Concentrated it with a protein concentrator since it has been diluted. The culture ...
adrian-protein.blogspot.com
Dimension: JUNE-JULY
http://adrian-protein.blogspot.com/2010/07/june-july.html
The X-ray study of proteins is sometimes regarded as an abstruse subject comprehensible only to specialists, but the basic ideas underlying our work are so simple that some physicists find them boring. (Perutz 1992). Friday, July 30, 2010. Was trying to produce more protein from the 1.6L induced bacterial cultures. Purified with Affinity chromatography successfully. (Although lost an amount of protein due to my mistakes). Concentrated it with a protein concentrator since it has been diluted.
