amplicot.ucsf.edu
amplicot.ucsf.eduAmpliCot -- using Cot analysis to measure PCR product sequence diversity
http://amplicot.ucsf.edu/
AmpliCot -- using Cot analysis to measure PCR product sequence diversity
http://amplicot.ucsf.edu/
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https://amplicot.ucsf.edu
AmpliCot -- using Cot analysis to measure PCR product sequence diversity
amplicot.ucsf.edu
AmpliCot protocols
http://amplicot.ucsf.edu/protocols.htm
Http:/ amplicot.ucsf.edu. Below are links to the most recent protocols. Ordering information for supplies (Excel). General AmpliCot protocol (pdf). TCR AmpliCot protocol (pdf). TCR Primer sequences (Excel). PCR and annealing programs. Last updated September 5, 2006.
AmpliCot forum
http://amplicot.ucsf.edu/forum.htm
Http:/ amplicot.ucsf.edu. Future home of the AmpliCot forum! We are still constructing this website, but in the future we hope to host a discussion forum on the AmpliCot method. Until the forum is established, please send any comments or queries you would like to share directly to us. And we will post them on this site.
AmpliCot publications
http://amplicot.ucsf.edu/publications.htm
Http:/ amplicot.ucsf.edu. Below are links to publications describing or using the AmpliCot method. Direct measurement of T-cell receptor repertoire diversity with AmpliCot. Baum PD, McCune JM. Nature Methods. 2006 Nov;3(11):895-901. News and views pdf. Erratum: Labels for naive and memory cells were reversed in Figure 6. Design, construction, and validation of a modular library of sequence diversity standards for polymerase chain reaction. Baum PD, Young JJ, Zhang Q, Kasakow Z, McCune JM.
AmpliCot FAQ
http://amplicot.ucsf.edu/faq.htm
Http:/ amplicot.ucsf.edu. What equipment would I need to start using AmpliCot? How much does it cost per sample to perform the AmpliCot assay? How difficult is it to learn how to perform the AmpliCot assay? Why bother with hybridization kinetics in the era of high-throughput sequencing? How could the AmpliCot assay be improved in the future? How does AmpliCot compare to other methods for evaluating TCR diversity? How much does it cost per sample to use AmpliCot to measure TCR diversity? I was able to tea...
Welcome to amplicot.ucsf.edu
http://amplicot.ucsf.edu/welcome.htm
Http:/ amplicot.ucsf.edu. This site is devoted to AmpliCot - a new method we have invented. AmpliCot provides a way to measure the sequence diversity of PCR products without requiring any sequencing. AmpliCot is based on the principle of Cot analysis, also known as DNA hybridization kinetics, first developed by Britten and colleagues. We've taken this method, automated it, and solved many of the problems inherent in applying it to PCR products. Paul Baum and Mike McCune.
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