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Antibodies that work

Friday, June 12, 2015. The Antibody Two-Step Solution. In the past year there have been a number of articles in Nature and other major journals that discuss the antibody problem in somewhat apocalyptic terms. In my mind there are only two main issues in the antibody problem: antibody validation and antibody variability. Both of these issues have straightforward solutions that do not require any massive influx of cash or massive restructuring of antibody production. The first is that once an antibody is f...

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Antibodies that work | antibodiesthatwork.blogspot.com Reviews

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Friday, June 12, 2015. The Antibody Two-Step Solution. In the past year there have been a number of articles in Nature and other major journals that discuss the antibody problem in somewhat apocalyptic terms. In my mind there are only two main issues in the antibody problem: antibody validation and antibody variability. Both of these issues have straightforward solutions that do not require any massive influx of cash or massive restructuring of antibody production. The first is that once an antibody is f...

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Antibodies that work | antibodiesthatwork.blogspot.com Reviews

https://antibodiesthatwork.blogspot.com

Friday, June 12, 2015. The Antibody Two-Step Solution. In the past year there have been a number of articles in Nature and other major journals that discuss the antibody problem in somewhat apocalyptic terms. In my mind there are only two main issues in the antibody problem: antibody validation and antibody variability. Both of these issues have straightforward solutions that do not require any massive influx of cash or massive restructuring of antibody production. The first is that once an antibody is f...

INTERNAL PAGES

antibodiesthatwork.blogspot.com

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Antibodies that work: May 2013

http://www.antibodiesthatwork.blogspot.com/2013_05_01_archive.html

Tuesday, May 7, 2013. Antibodies That DO NOT WORK in Western Blots. What does it mean when an antibody company says an antibody “does not work” in western blots? I started wondering about this question the other day after a colleague told me about a company that “validates” all of their antibodies by immunostaining in no less than 5 different tissues! We examined the company’s website and in some cases western blots (WB) showing labeling of a single band were also presented. What does this mean? The lane...

2

Antibodies that work

http://www.antibodiesthatwork.blogspot.com/2012/07/antibody-specificity-use-of-blocking.html

Tuesday, July 24, 2012. Antibody specificity: The use of a blocking control has only limited value. Antibody specificity is one of the key issues in determining whether you have an antibody that works. How does one determine that the antibody specifically recognizes only the target of interest? There are a number of control procedures one can use to be sure that the signal generated in the antibody based assay truly and quantitatively represents the presence of the target of interest. July 26, 2012 at 6:...

3

Antibodies that work: October 2013

http://www.antibodiesthatwork.blogspot.com/2013_10_01_archive.html

Thursday, October 10, 2013. In a recent article in Nature Methods, Vivien Marx discussed some of the key issues relating to the use of antibodies http:/ tinyurl.com/osygswn. Variability in the quality of the antibodies available commercially was one of the principal problems encountered by scientists in her article. This problem is closely related to the larger issue addressed in numerous editorials in Nature and other journals regarding the irreproducibility of research. So this is one huge contributor ...

4

Antibodies that work: April 2015

http://www.antibodiesthatwork.blogspot.com/2015_04_01_archive.html

Monday, April 13, 2015. Why Recombinant Proteins Make Poor Antibody Validation Tools. Antibody validation is a topic that has garnered a great deal of attention lately in discussions of the problem with the lack of reproducibility in science. One type of antibody validation that should be avoided uses purified recombinant proteins. In a typical study of this type a purified recombinant protein is run on a western blot and then the labeling of the new antibody is examined. In a tissue of interest? Neverth...

5

Antibodies that work: Antibody Variability

http://www.antibodiesthatwork.blogspot.com/2013/10/antibody-lot-to-lot-variability.html

Thursday, October 10, 2013. In a recent article in Nature Methods, Vivien Marx discussed some of the key issues relating to the use of antibodies http:/ tinyurl.com/osygswn. Variability in the quality of the antibodies available commercially was one of the principal problems encountered by scientists in her article. This problem is closely related to the larger issue addressed in numerous editorials in Nature and other journals regarding the irreproducibility of research. So this is one huge contributor ...

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antibodiesthatwork.blogspot.com

Antibodies that work

Friday, June 12, 2015. The Antibody Two-Step Solution. In the past year there have been a number of articles in Nature and other major journals that discuss the antibody problem in somewhat apocalyptic terms. In my mind there are only two main issues in the antibody problem: antibody validation and antibody variability. Both of these issues have straightforward solutions that do not require any massive influx of cash or massive restructuring of antibody production. The first is that once an antibody is f...

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