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BioFreaks Biochemistry Blog (v.Beta) | bio-ggs.blogspot.com Reviews
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Always looking for answers...
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BioFreaks Biochemistry Blog (v.Beta) | bio-ggs.blogspot.com Reviews

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BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Cloning

https://bio-ggs.blogspot.com/2009/11/ggs-live-cloning.html

Sunday, 22 November 2009. GGS LIVE - Cloning. What is cloning you ask! I see cloning as set of methods which allow to copy specyfic piece of DNA and then to use it as we wish. Amplification of three different cDNAs (cDNA is a protein coding part of the gene for more informations see this amnimation McGraw Hill Animations - cDNA (complementary DNA). By PCR and transfering them (cloning) into first transient vector and afterwards to a final (target) vector. Each of our cDNAs is around 300bp. As you can see...

2

BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Antibody Generation

https://bio-ggs.blogspot.com/2015/05/ggs-live-antibody-generation.html

Saturday, 2 May 2015. GGS LIVE - Antibody Generation. Today in the GGS LIVE section we will cover how to generate rabbit polyclonal antibody:). Established methodology for antibody generation is an essential tool to study cellular functions of the protein of interest. Antibodies are generated by injection of the antigen. Into host animal (mouse, rat, rabbit etc) and subsequent isolation of antibodies from the animal serum. For instance from rabbit serum). Generation of antibody against protein X. Once we...

3

BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Polymerase chain reaction (PCR)

https://bio-ggs.blogspot.com/2011/01/ggs-live-polymerase-chain-reaction-pcr.html

Wednesday, 26 January 2011. GGS LIVE - Polymerase chain reaction (PCR). Polymerase chain reaction (PCR). PCR allows for amplification of a particular DNA sequence. Ampliifcation of a gene X sequence from genomic DNA. Ok lets start. Polymerase chain reaction is a commonly used technique. The theory behind it as well aas practical part of PCR are very simple. For PCR reaction we need:. Thermostabile polymerase - which can stand temperature up to 95 degrees Celsius,. DNA template - contains target DNA,.

4

BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Site Directed Mutagenesis

https://bio-ggs.blogspot.com/2011/06/ggs-live-site-directed-mutagenesis.html

Monday, 13 June 2011. GGS LIVE - Site Directed Mutagenesis. Today in the GGS - LIVE section the Site Directed Mutagenesis technique. Wanna see how it is done? Site Directed Mutagenesis (SDM). Allows for introduction of point mutations in DNA and thus sometimes in protein sequence. Introduction of STOP codon in order to generate C-terminal deletion in protein X. Site directed mutagenesis is performed on circular DNA substrate. In our study case, the cDNA. You probably, wonder why SDM product and template ...

5

BioFreaks Biochemistry Blog (v.Beta): GGS LIVE - Making a fusion protein

https://bio-ggs.blogspot.com/2010/11/ggs-live-making-fusion-protein.html

Wednesday, 17 November 2010. GGS LIVE - Making a fusion protein. Today in the GGS LIVE section we will learn how to virtually design and generate a fusion protein. Protein tagging allows to perform specific experimenents that are not possible with the endogenous protein (wild-type protein). Desing and generation of protein X fusion with a GFP (green fluorescent protein) tag. Easily detect our protein, we would use epitope tags like myc, V5 or HA,. The red triplets are start and stop codons, respectively&...

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Stringent Response: December 2011

http://stringentresponse.blogspot.com/2011_12_01_archive.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Wednesday, December 14, 2011. Single molecule tracking fluorescence microscopy in mitochondria reveals highly dynamic but confined movement of Tom40. Kuzmenko et al., Scientific Reports (2011) 1:95. Friday, December 2, 2011. Long memories of RelA. Enzymes have their...

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Stringent Response: September 2012

http://stringentresponse.blogspot.com/2012_09_01_archive.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Friday, September 14, 2012. PpGpp regulates GTP synthesis by inhibiting Gmk and HprT. Another role for ppGpp was discovered by Allison Kriel and colleagues: it directly regulates GTP levels by interfering with GTP biosynthetic pathway. So the connection between.

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O mnie | leny.pl

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Witaj w świecie Lenego :). Jestem Leny. Lubię maincrafta, spore, lego przygodę i inne gry, bajki i zabawy z kolegami. No i chyba jeszcze Kindzie-Mindzie. Jak uroczy jest świat widziany oczyma dziecka, chociaż wspomagany techniką fotograficzną jego mamy. Dostrzegamy istotne akcenty małego chłopca – Lenusia, dla którego otoczenie, to potrzeba ciągłej zabawy. Dodaj komentarz Anuluj pisanie odpowiedzi. Twój adres e-mail nie zostanie opublikowany. Możesz użyć następujących tagów oraz atrybutów.

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Stringent Response: GDP and SRL don't mix

http://stringentresponse.blogspot.com/2012/11/gdp-and-srl-dont-mix-well.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Thursday, November 15, 2012. GDP and SRL don't mix. This suggests a neat mechanism for the destabilisation of the ribosome-bound GDP form of the GTPase: the moor has done his duty, the moor can go. Mitkevich et al., Scientific Reports 2012 2:843, PIMD: 32150791.

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Stringent Response: Two PhD and one postdoctoral position at MIMS

http://stringentresponse.blogspot.com/2013/03/two-phd-and-one-postdoctoral-position_30.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Saturday, March 30, 2013. Two PhD and one postdoctoral position at MIMS. Our lab is branching out. This fall we cross the Baltic sea and set up a new lab in Umeå. Sweden - at the Laboratory for Molecular Infection Medicine Sweden, MIMS. Do you like ribosomes? Post d...

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Stringent Response: Postdoctoral position, 24 months with a possibility of extension

http://stringentresponse.blogspot.com/2015/02/postdoctoral-position-24-months-with.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Monday, February 9, 2015. Postdoctoral position, 24 months with a possibility of extension. The Laboratory for Molecular Infection Medicine Sweden (MIMS) within the Nordic EMBL Partnership for Molecular Medicine. Umeå University, Sweden. Or Dr Gemma C. Atkinson,.

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Stringent Response: February 2015

http://stringentresponse.blogspot.com/2015_02_01_archive.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Monday, February 9, 2015. Postdoctoral position, 24 months with a possibility of extension. The Laboratory for Molecular Infection Medicine Sweden (MIMS) within the Nordic EMBL Partnership for Molecular Medicine. Umeå University, Sweden. Or Dr Gemma C. Atkinson,.

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Stringent Response: Tet(O) cryoEM structure is out!

http://stringentresponse.blogspot.com/2013/02/teto-cryoem-structure-is-out.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Thursday, February 14, 2013. Tet(O) cryoEM structure is out! So it is finally out: the cryoEM structure of Tet(O) on the ribosome. We have collaborated on with Joachim Frank's lab. Recently cryoEM of Tet(O)'s close relative,. Dönhöfer at al. PNAS (2012) PIMD:. BioFr...

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Stringent Response: January 2012

http://stringentresponse.blogspot.com/2012_01_01_archive.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Wednesday, January 4, 2012. PhD student in Molecular Evolution with Dr. Gemma Atkinson. Is looking for a PhD student in Molecular Evolution. The candidate should have:. A Masters degree in a biological or computational discipline. A statement of academic interests.

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Stringent Response: November 2012

http://stringentresponse.blogspot.com/2012_11_01_archive.html

Here I muse about stuff directly or not-so-directly related to what we do in my lab. There are two types of posts: streamlined ones for researchblogging.org and not-so-streamlined ones for brain dump. I use labels - do take advantage of that! Thursday, November 15, 2012. GDP and SRL don't mix. This suggests a neat mechanism for the destabilisation of the ribosome-bound GDP form of the GTPase: the moor has done his duty, the moor can go. Mitkevich et al., Scientific Reports 2012 2:843, PIMD: 32150791.

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BioFreaks Biochemistry Blog (v.Beta)

Saturday, 2 May 2015. GGS LIVE - Antibody Generation. Today in the GGS LIVE section we will cover how to generate rabbit polyclonal antibody:). Established methodology for antibody generation is an essential tool to study cellular functions of the protein of interest. Antibodies are generated by injection of the antigen. Into host animal (mouse, rat, rabbit etc) and subsequent isolation of antibodies from the animal serum. For instance from rabbit serum). Generation of antibody against protein X. Once we...

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Sexta-feira, 14 de fevereiro de 2014. Jogos sobre s Célula Animal e célula Vegetal. Click nos links abaixo. Http:/ ticsnaeducacao.com.br/index.php? Http:/ www.sobiologia.com.br/jogos/popupJogo.php? Http:/ br.syvum.com/cgi/online/mult.cgi/materia/biologia/cellgr7.tdf? Http:/ rachacuca.com.br/quiz/29921/biologia-celulas-tecidos-e-sistemas/. Compartilhar com o Pinterest. Quinta-feira, 25 de agosto de 2011. Duplicação do DNA, Transcrição e Tradução. 2-Transcrição e tradução de um gene:. Http:/ campus.for...