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Welcome to the Biology 305 Lab - Biology 305 Laboratory

Dept of Biology, University of Regina. Preparing for the lab. Pipetters use and care. Introduction to the lab. Welcome to the Biology 305 Lab. The 305 lab does not lend itself well to a flat document because we frequently find it necessary to jump around in the lab manual and many students find this frustrating. We hope this new online lab manual will be more user-friendly. We will give you a printed copy protocol book. You can also find a link to the protocol book in the side menu. Also - remember to se...

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Welcome to the Biology 305 Lab - Biology 305 Laboratory | bio305lab.wikidot.com Reviews

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Dept of Biology, University of Regina. Preparing for the lab. Pipetters use and care. Introduction to the lab. Welcome to the Biology 305 Lab. The 305 lab does not lend itself well to a flat document because we frequently find it necessary to jump around in the lab manual and many students find this frustrating. We hope this new online lab manual will be more user-friendly. We will give you a printed copy protocol book. You can also find a link to the protocol book in the side menu. Also - remember to se...

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Welcome to the Biology 305 Lab - Biology 305 Laboratory | bio305lab.wikidot.com Reviews

https://bio305lab.wikidot.com

Dept of Biology, University of Regina. Preparing for the lab. Pipetters use and care. Introduction to the lab. Welcome to the Biology 305 Lab. The 305 lab does not lend itself well to a flat document because we frequently find it necessary to jump around in the lab manual and many students find this frustrating. We hope this new online lab manual will be more user-friendly. We will give you a printed copy protocol book. You can also find a link to the protocol book in the side menu. Also - remember to se...

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bio305lab.wikidot.com

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Week 5 - Biology 305 Laboratory

http://bio305lab.wikidot.com/weeklyguide2014:week5

Dept of Biology, University of Regina. Preparing for the lab. Intro lab Sept. 9/10. Pipetters use and care. Introduction to the lab. With the ligation products. An easy way to make copies of the products is to use. To replicate them. We get the ligation products into the. Cells using transformation. Earlier this semester, we made competent. Cells This week we will transform some of our stored competent cells with the ligation reactions from last week. Following transformation the. Details on how to set up.

2

Week 4 - Biology 305 Laboratory

http://bio305lab.wikidot.com/weeklyguide2014:week4

Dept of Biology, University of Regina. Preparing for the lab. Intro lab Sept. 9/10. Pipetters use and care. Introduction to the lab. Gel-purify Gene A and set up ligations. NOTE Each group needs to come in before the lab period to prepare an agarose gel (1 gel per group details below). In previous labs we prepared the vector (pUC18) for both ligations, and we prepared pKC7 for the shotgun ligation. Read before coming to the lab. Step III - IV Produce and prepare the DNA fragments for cloning and Ligation.

3

Agarose gel electrophoresis - Biology 305 Laboratory

http://bio305lab.wikidot.com/resources:agarosegelelect

Dept of Biology, University of Regina. Preparing for the lab. Intro lab Sept. 9/10. Pipetters use and care. Introduction to the lab. We will use agarose gel electrophoresis to determine DNA fragment sizes, and to quantify DNA. Following electrophoresis the DNA is visualized and the gel is photographed. The distance each DNA fragment moves from the gel origin is directly proportional to the size of the DNA fragment. If a DNA ladder. What buffer to use. The concentration of agarose to use. We commonly refe...

4

Practice Problems - Biology 305 Laboratory

http://bio305lab.wikidot.com/practice-problems

Dept of Biology, University of Regina. Preparing for the lab. Intro lab Sept. 9/10. Pipetters use and care. Introduction to the lab. Working with stock solutions. DNA mass amounts versus concentrations. Number of molecules versus mass. Working with stock solutions. DNA mass amounts versus concentrations. Number of molecules versus mass. Beginning molecular biologists have a hard time with the concept of equal numbers of molecules versus equal mass. Powered by Wikidot.com. Check out how this page has evol...

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Week 7 - Biology 305 Laboratory

http://bio305lab.wikidot.com/weeklyguide2014:week7

Dept of Biology, University of Regina. Preparing for the lab. Intro lab Sept. 9/10. Pipetters use and care. Introduction to the lab. Analysis of recombinants via restriction mapping - Part 2. Previously we used the restriction maps for the vector and insert DNA to determine the fragments we should see when we digest the new recombinants with. Last week you digested several recombinant plasmids with these enzymes. BEFORE THE LAB each group needs to come in a prepare 2 - 25 ml agarose gels. If there are an...

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Bio 301D – A course about making decisions, evaluating information, and knowing what to trust. A course about making decisions, evaluating information, and knowing what to trust. The Scientific Method (1-3). Chapter 1: WHY SCIENCE MATTERS. Chapter 2: A TEMPLATE FOR SCIENTIFIC INQUIRY. Chapter 3: HOW NON-SCIENTISTS USE THE SCIENTIFIC METHOD. Chapter 4: MODELS ARE THE BUILDING BLOCKS OF SCIENCE. Chapter 5: ALL MODELS ARE FALSE. BUT SOME ARE USEFUL ANYWAYS. Chapter 6: MODELS OF SEX IN CONDOM TESTING. Final ...

bio305lab.wikidot.com

Welcome to the Biology 305 Lab - Biology 305 Laboratory

Dept of Biology, University of Regina. Preparing for the lab. Pipetters use and care. Introduction to the lab. Welcome to the Biology 305 Lab. The 305 lab does not lend itself well to a flat document because we frequently find it necessary to jump around in the lab manual and many students find this frustrating. We hope this new online lab manual will be more user-friendly. We will give you a printed copy protocol book. You can also find a link to the protocol book in the side menu. Also - remember to se...

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Biology 30 @ HT YMM

Biology 30 @ HT YMM. Wednesday, 22 April 2015. Update - April 22. My apologies for such a delay in posting. We are moving along quite nicely in Biology 30 as the weather warms up and we head into the homestretch of the school year. Today, students are completing the final assessment for Unit C1: Cell Division. This has been a relatively short unit, so the assessment is a bit shorter and not worth quite as much as our previous tests. Most often, this unit is reported as the most interesting. For homework ...

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Mr C's "popular" Bio30 BLOG. This is a transcript of our classes learning during the Fall 2007 semester at Sundre High School. It is intended to assist students who were absent, and reinforce the value of daily review of class material for concept acquisition. Comments are welcome. Wednesday, June 3, 2009. Mac Walton- June 3- 3/3 Blog #2. We had a super badass. Quiz first up on the endocrine system. Questions out of key/ reviewbook. We ascertained a review 'guide' sheet. Bed, Bath and Beyond. To your dis...

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