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上海宝吉生物技术有限公司 | biogenesci.com Reviews
<META>
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上海宝吉生物技术有限公司
<META>
KEYWORDS
1 关键词
2 酵母双杂交文库构建
3 酵母单杂交文库构建
4 酵母双杂交文库筛选
5 酵母单杂交文库筛选
6 免疫组化
7 动物实验
8 课 题外包
9 蛋白子组测序
10 iTRAQ
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关于我们,文库构建,核蛋白酵母双杂交文库构建服务,膜蛋白酵母双杂交文库构建技术服务,酵母单杂交文库构建服务,invitrogen gateway系统酵母双 单 杂交文库构建,酵母双杂交文库筛选技术服务,酵母单杂交文库筛选技术服务,均一化cdna文库构建,全长cdna文库构建,fosmid文库构建服务,抑制性消减杂交 ssh 文库构建,蛋白质组,silac技术服务,itraq测序,基因编辑,基因组crispr敲除文库,全基因组获得性功能的筛选文库,课题服务,整体课题技术服务,动物实验,动物模型,基因测序
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上海宝吉生物技术有限公司 | biogenesci.com Reviews

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1

Invitrogen-Gateway系统酵母双(单)杂交文库构建-上海海科生物技术有限公司

http://www.biogenesci.com/product/277601879

Pathway-focused CRISPR gRNA 文库. 热烈祝贺 上海海科生物 与 上海宝吉生物 正式合并-强强联合. 3 使用gateway高效率的片段和载体重组连接方法,连接效率很高,可以大大提高原始文库的库容量 可以达到1*10 7 CFU 以上的原始文库库容量。 3 使用gateway高效率的片段和载体重组连接方法,连接效率很高,可以大大提高原始文库的库容量 可以达到1*10 7 CFU 以上的原始文库库容量。 4 该系统的文库会有初级文库提供,初级文库的载体为pDONR221,可以方便地将该样本的文库再构建到其他任意载体上,形成其他用途的文库,比如 pPR3-N、PET22b、PET19b、pYD1、pPRC9K、pLenti6.3、pET32b、pDest52、pDEST17等任意载体。 29 初级文库质粒与酵母文库载体pGADT7 pGADT7-Rec2)进行gateway LR重组,形成次级文库。 31 文库库容量 大于1*10 7CFU。 32 平均插入片段 大于1.2Kbp。 5]W Maa,S. Kilaru a,C. Collins, Libraries ...6]Men...

2

iTRAQ测序-上海海科生物技术有限公司

http://www.biogenesci.com/product/277485816

Pathway-focused CRISPR gRNA 文库. 热烈祝贺 上海海科生物 与 上海宝吉生物 正式合并-强强联合. ITRAQ (Isobaric Tags for Relative and Absolute Quantitation)即等重标签标记用于蛋白质相对和绝对定量技术。 ITRAQ试剂包括3个部分 报告基团 report group 、平衡基团 balance group 和肽反应基团 amine-specific reactive group。 ITRAQ 可于一次实验中同时标记 4 至 8 个的样品,操作简单快速,减少多次质谱检测中产生的系统误差。 Triple TOF 5600, Q-Exactive, Orbitrap Fusion Tribrid.

3

酵母单杂交文库构建服务-上海宝吉生物技术有限公司

http://www.biogenesci.com/酵母单杂交文库构建

Pathway-focused CRISPR gRNA 文库. 热烈祝贺 上海海科生物 与 上海宝吉生物 正式合并-强强联合. 我们承诺的文库各项指标在国内各家公司里是最高的 平均插入片段 1200bp 比市场上其他公司高出200bp以上 文库原始库容量 1*10 7CFU 比市场上其他公司高出10倍以上 文库空载率 5%。 服务报价 3万元整,如果需要转化到酵母,增加4000.00元,如果需要增加均一化处理,增加3000.00元,免费赠送3个读码框处理,以确保蛋白可以正常表达。 上海海科结合多年的文库构建经验,强势推出 All-Direct 文库构建技术,我们的产品较其他构建方法 主要有takara的SAMRT方法和invitrogen的gateway方法 ,技术和质量上都具有极大的优势。 SMART方法使用酶切连接或者酵母细胞内的低效率重组的方式,进行片段与载体的连接,效率低下,假阳性率高,原始文库的库容在1*10 5CFU-1*10 6CFU.我们使用高效率的体外重组方式,大大提高重组效率,文库的原始库容大于1*10 7CFU. 3]Sonalika Mahajan, Gaurav Kumar S...

4

服务项目-上海宝吉生物技术有限公司

http://www.biogenesci.com/fwxm

Pathway-focused CRISPR gRNA 文库. 热烈祝贺 上海海科生物 与 上海宝吉生物 正式合并-强强联合.

5

基因组范围的CRISPR敲除(GeCKO)文库-上海海科生物技术有限公司

http://www.biogenesci.com/product/277485810

Pathway-focused CRISPR gRNA 文库. 热烈祝贺 上海海科生物 与 上海宝吉生物 正式合并-强强联合. 基因组范围的CRISPR 敲除 GeCKO 文库能够在人或小鼠细胞中快速产生功能缺失的突变体并且筛选所期望的表型。 要获取更多GeCKO文库设计、构建信息,请参考张峰实验室发表的文献 Sanjana N.E., Shalem O., Zhang F. Improved vectors and genome-wide libraries for CRISPR screening. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047. Human GeCKO Library A. Human GeCKO Library A. Human GeCKO Library B. Human GeCKO Library B. Mouse GeCKO Library A. Mouse GeCKO Library A. 周期为工作日 * 需求量可以100 µg为单位向上增加 欢迎咨询,发送邮件中请备注说明文库名称及相应的Cat.No.

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