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Virology: The Non-Living Biology | Just another WordPress.com weblog

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Virology: The Non-Living Biology | Just another WordPress.com weblog | biologyofviruses.wordpress.com Reviews
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Virology: The Non-Living Biology | Just another WordPress.com weblog | biologyofviruses.wordpress.com Reviews

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Cell Counting | Virology: The Non-Living Biology

https://biologyofviruses.wordpress.com/lab-expt/cell-counting

Virology: The Non-Living Biology. Just another WordPress.com weblog. Determine how many viable (living) cells are present in a cell culture using a haemocytometer. Is an instrument used to determine the number of cells per unit volume of a suspension. Remove medium from confluent cell flask. Wash with PBS solution. Then remove PBS. Add 1ml of Trypsin. Incubate at 37. C for 1 min. Add 9 ml of growth medium to form a cell suspension. Load mixture into both of the chambers of a haemocytometer. Viral Infecti...

2

Plaque Assay | Virology: The Non-Living Biology

https://biologyofviruses.wordpress.com/lab-expt/plaque-assay

Virology: The Non-Living Biology. Just another WordPress.com weblog. Counting viruses grown in cell culture using CMC and Crystal Violet. Stands for Carboxymethyl Cellulose. It causes the medium to be viscous (sticky). Is a purple dye. It stains viable (live) cells purple and leaves dead infected cells (plaques) unstained. Perform Serial Dilution using 0.5ml of Kunjin virus with 4.5ml of virus diluent. Remove all medium from 2 six well plates. Wash with Virus Diluent. Then remove Virus Diluent. Subcultur...

3

Lab Expt | Virology: The Non-Living Biology

https://biologyofviruses.wordpress.com/lab-expt

Virology: The Non-Living Biology. Just another WordPress.com weblog. Virology: Nasty Lab Concept. Welcome onboard ‘Virology: Nasty Lab Concept’ and we’ll find out more about the main principles behind some of the microbiology laboratory experiments. These experiments include:. Subculturing an Immortal Cell Line. Viral Infection and Cytopathic Effects. ELISA (Enzyme-Linked Immuno-Sorbent Assay). Do have fun learning and exploring =D. Leave a Reply Cancel reply. Enter your comment here.

4

Structure | Virology: The Non-Living Biology

https://biologyofviruses.wordpress.com/structure

Virology: The Non-Living Biology. Just another WordPress.com weblog. Virology: The Ingenious Architecture. Infectious agents that are unable to multiply outside a host cell. In general, most viruses are made up of two or three parts:. They have genes that are made from either DNA or RNA. A protein coat that protects these genes. And some of them have an envelope of fats. The viral capsids display a wide diversity of shapes and sizes, called morphologies. Leave a Reply Cancel reply. Enter your comment here.

5

Library | Virology: The Non-Living Biology

https://biologyofviruses.wordpress.com/library

Virology: The Non-Living Biology. Just another WordPress.com weblog. Virology: The Viral iTUNES. Welcome to Virology: The Non-Living Biology Library Portal. More to Come! And soon there will be an A to Z list of viruses stored in this database. The different types of viruses have been classified accordingly for easy reference. =). Plus-strand RNA and Double-Strand RNA viruses. Viruses Whose Life Cycle Uses Reverse Transcriptase. Leave a Reply Cancel reply. Enter your comment here. Hepatitis B Virus (HBV):.

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Virology: The Non-Living Biology. Just another WordPress.com weblog. February 2, 2009 – 6:35 pm. Whenever a new chapter is added to the blog site, announcements will be made through this main page. January 23, 2009 – 3:32 am. Wonder how viruses spread, live and parasite on you when you’re ill? Then this is the right place for you! Hover your mouse around and venture into Virology: The Non-Living Biology…. Subculturing an Immortal Cell Line. Viral Infection and Cytopathic Effect. Hepatitis B Virus (HBV):.

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