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ISMB-Biophysics Centre | biophysics.ismb.lon.ac.uk Reviews
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ISMB-Biophysics Centre | biophysics.ismb.lon.ac.uk Reviews

https://biophysics.ismb.lon.ac.uk

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biophysics.ismb.lon.ac.uk biophysics.ismb.lon.ac.uk
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ISMB-Biophysics Centre

http://biophysics.ismb.lon.ac.uk/calorimetry.html

Institute of Structural and Molecular Biology Home. The ISMB Biophysics Centre. A wide range of buffer conditions are suitable. A further advantage is that molecules do not need to be labelled or altered, so the method lends itself to investigations at native conditions. However, a relatively large sample volume is required, so alternatively Surface Plasmon Resonance. Could be used, or, if one of the binding partners is fluorescent, anisotropy measurements. Also for DSC, the solution conditions can be re...

2

ISMB-Biophysics Centre

http://biophysics.ismb.lon.ac.uk/kinetics.html

ISMB research programmes Structural Biology. The ISMB Biophysics Centre. KinetAsysT, Hi-Tech Scientific). Various size syringes are available to allow mixing at different ratios. Double-mixing of samples is also possible to further investigate reactions of more than one step. The syringes and optical cell are immersed in a thermostatted waterbath allowing choice of reaction temperature. For initial enzyme characterisation classical turnover experiments are very important. These give direct informatio...

3

ISMB-Biophysics Centre

http://biophysics.ismb.lon.ac.uk/cd.html

ISMB research programmes Structural Biology. The ISMB Biophysics Centre. Model 202SF, Aviv Biomedical). Circular dichroism is the property of chiral molecules to absorb the right and left components of circularly polarised light to a different extent. CD spectroscopy measures this differential absorbance. ISMB Biophysics Centre, Birkbeck, University of London.

4

ISMB-Biophysics Centre

http://biophysics.ismb.lon.ac.uk/fluorimetry.html

ISMB research programmes Structural Biology. The ISMB Biophysics Centre. Our FluoroMax3 has a stirred and temperature-controlled 4-position sample changer. Alternatively, a fibre-optic 96/384 microwell-plate reader can be connected for high-throughput applications, e.g. screening of potential ligands or rapid collection of concentration-dependent data. ISMB Biophysics Centre, Birkbeck, University of London.

5

ISMB-Biophysics Centre

http://biophysics.ismb.lon.ac.uk/home.html

ISMB research programmes Structural Biology. The ISMB Biophysics Centre. The ISMB Biophysics Centre provides access to instrumentation and expertise in the analysis of biomolecular interactions for scientists in London. A variety of instrumentation is available for the characterisation of equilibrium and kinetic behaviours of protein, nucleic acid and small-molecule interactions on a hire, service or collaborative basis. For an overview of the methods and techniques available, please click here. School o...

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Institute of Structural Molecular Biology - ISMB

http://www.ismb.lon.ac.uk/biophysics.html

ISMB research programmes Structural Biology. Bioinformatics Biochemistry and Molecular Biology. The ISMB biophysics programme studies the behaviour of biological macromolecules in solution and aims to elucidate the mechanisms and energetics of interactions between them. To carry out such investigations, we use a broad spectrum of techniques including microcalorimetry, fluorescence spectroscopy, analytical ultracentrifugation, circular dichroism, and surface plasmon resonance.

ismb.lon.ac.uk ismb.lon.ac.uk

Institute of Structural Molecular Biology - ISMB

http://www.ismb.lon.ac.uk/facilities.html

ISMB research programmes Structural Biology. Biochemistry and Molecular Biology. In each of the programme areas. The ISMB seeks to create or promote the creation of a research centre. These research centres will provide an interface with and services for the wider community of biomedical researchers at UCL, in London and nationally. The ISMB has state of the art facilities in electron microscopy, x-ray crystallography and NMR. The ISMB Biophysics Centre. The Bloomsbury Centre for Bioinformatics (BCB).

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ISMB research programmes Structural Biology. The ISMB Biophysics Centre. The ISMB Biophysics Centre provides access to instrumentation and expertise in the analysis of biomolecular interactions for scientists in London. A variety of instrumentation is available for the characterisation of equilibrium and kinetic behaviours of protein, nucleic acid and small-molecule interactions on a hire, service or collaborative basis. For an overview of the methods and techniques available, please click here. School o...

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