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biosensorcore.com

Biosensor Core

Biacore 3000 and T200 biosensor facility at the University of Maryland

http://www.biosensorcore.com/

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Biosensor Core | biosensorcore.com Reviews
<META>
DESCRIPTION
Biacore 3000 and T200 biosensor facility at the University of Maryland
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KEYWORDS
1 biosensor core
2 overview
3 introduction
4 surface plasmon resonance
5 special features
6 getting started
7 ligand capture
8 yes no binding
9 optimized binding
10 typical studies
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biosensor core,overview,introduction,surface plasmon resonance,special features,getting started,ligand capture,yes no binding,optimized binding,typical studies,antibody specificity,kinetic analysis,weak binding,competition,sequential binding,caveats,fees
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Biosensor Core | biosensorcore.com Reviews

https://biosensorcore.com

Biacore 3000 and T200 biosensor facility at the University of Maryland

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1

Yes-No Binding | Biosensor CoreBiosensor Core

http://biosensorcore.com/typical-procedures/yes-no-binding

The first step in using Biacore. Instrumentation to measure binding is to determine if binding between two molecules can be detected. This is termed “yes-no” binding. The results show “yes” binding for the analyte shown in green, “no” for the analyte shown in pink. Red shows the buffer control.

2

Testing the Specificity of an Antibody for a Protein | Biosensor CoreBiosensor Core

http://biosensorcore.com/typical-procedures/testing-the-specificity-of-an-antibody-for-a-protein

Testing the Specificity of an Antibody for a Protein.

3

Binding of Small Molecules | Biosensor CoreBiosensor Core

http://biosensorcore.com/typical-procedures/small-molecules

Binding of Small Molecules.

4

Ligand Capture | Biosensor CoreBiosensor Core

http://biosensorcore.com/typical-procedures/ligand-capture

Before any studies can be performed on a Biacore. Instrument, the chip that provides the surfaces on which binding will take place must first be prepared by attaching the ligand (e.g., an antibody, a protein, DNA, a lipid monolayer). This is called “ligand capture.” The mode of ligand capture will depend on the nature of the ligand and the molecule to which you wish to measure its binding, called the “analyte.”. Or if it is biotinylated and you are using the Streptavidin (SA) chip.

5

Access | Biosensor CoreBiosensor Core

http://biosensorcore.com/typical-procedures/access

If you want to learn to use the instrument, you should obtain hands-on training, offered by the General Electric Corporation’s Biacore. Group in New Brunswick, N.J., several times a year. Inquire at this website. Our Core operator, Dr. Zhang, will check you out on our own instrument when you first sign up to use it. Alternatively, the facility can run your samples for you.

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Biosensor Core

Instrumentation is available for your use in the Biosensor Core Facility maintained by the Department of Physiology, University of Maryland School of Medicine. Biacore. Provides a versatile, highly sensitive, easy-to-use, and quantitative approach to study the kinetics and affinity of binding interactions under exquisitely controlled conditions. The technology relies on “ surface plasmon resonance. Proudly powered by WordPress. Theme: Yoko by Elmastudio.

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