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Bio-technique: Types of microscopes
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Sunday, December 26, 2010. There are several types of microscopes available on the market, selection of the proper type is not a simple assignmen as you would need to determine what exactly it would be used for. Below you can see all the types of modern microscopes for any scientific and hobby task. Also called " light microscope. Or " epifluorescent microscope. Is one of the most advanced and important types of microscopes with the highest magnifying capacity. In electron microscopes electrons are u...
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Bio-technique: INDOLE PRODUCTION
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Sunday, December 26, 2010. Indole is an aromatic heterocyclic organic compound that can be produced by some bacteria as a degradation product. Of the amino acid tryptophan. Tryptophanase is the enzyme involved tryptophan degradation resulting indole, pyruvate,. And ammonia. Indole test is most used for coliforms identification. Simplest medium for indole testing is Buffered peptone water (Proteose peptone 10g, NaCl 5g, Disodic phosphate 3.5g, Monopotasic phosphate 1.5g, H. How to Use a Hemacytometer.
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Bio-technique: RFLP Technique
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Sunday, December 26, 2010. In 1985, Sir Alec Jeffreys developed restriction fragment length polymorphism (RFLP), which quickly became the standard technique for DNA testing throughout the 1980s. RFLP provided the world with the first form of genetic testing based on DNA, the body's genetic material. Using RFLP, scientists cut specific portions of DNA into fragments for comparison. These fragments have different lengths, depending on the location of certain markers for cutting the DNA. In some RFLP cases,...
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Bio-technique: Bacteria - Identifying And Classifying Bacteria
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Sunday, December 26, 2010. Bacteria - Identifying And Classifying Bacteria. The most fundamental technique for classifying bacteria is the gram stain, developed in 1884 by Danish scientist Christian Gram. It is called a differential stain because it differentiates among bacteria and can be used to distinguish among them, based on differences in their cell wall. The walls of gram positive bacteria (for example, Staphylococcus aureus. Gram negative bacteria (e.g., Escherichia coli. Have thinner walls, cont...
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Bio-technique: OXIDASE TEST
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Thursday, December 30, 2010. Some bacteria like Pseudomonas, Pasteurellaceae, Campylobacter, Neisseria, Moraxella, can produce cytochrome C oxidase located in their membrane which can catalyse the transport of electrons from donor compounds to electron acceptors (oxygen). This respiratory system is present in aerobic bacteria which are capable to use oxygen as the final hydrogen receptor. Subscribe to: Post Comments (Atom). How to Use a Hemacytometer. Bacteria - Identifying And Classifying Bacteria.
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Bio-technique: 12/26/10 - 1/2/11
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Thursday, December 30, 2010. Some bacteria like Pseudomonas, Pasteurellaceae, Campylobacter, Neisseria, Moraxella, can produce cytochrome C oxidase located in their membrane which can catalyse the transport of electrons from donor compounds to electron acceptors (oxygen). This respiratory system is present in aerobic bacteria which are capable to use oxygen as the final hydrogen receptor. Links to this post. Sunday, December 26, 2010. The degree of variation among HLA in different people provides for fai...
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Bio-technique: How to Use a Hemacytometer
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Sunday, December 26, 2010. How to Use a Hemacytometer. How to use a hemacytometer. French physiologist Louis-Charles Malassez (1842-1909) studied a lot of things in his life. In dentistry, the residual cells of the epithelial root sheath in the periodontal ligament are now called the epithelial rests of Malassez. A genus of fungi is also named for him, which includes species that can cause dandruff and other skin infections. 1 Transfer a small sample of cell suspension ( 100 μl) to a microfuge tube.
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Bio-technique: PCR Technique
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Sunday, December 26, 2010. In 1983, Kary Mullis and members of the human genetics team at Cetus Corporation developed a genetic replication technique called polymerase chain reaction (PCR). After several years of fine-tuning the process, PCR. Became the most popular DNA replication technique by the 1990s. Kary Mullis was awarded the Nobel Prize in Chemistry for this work in 1993. In PCR, scientists isolate a small amount of DNA (an amount easily obtained from a buccal swab. How to Use a Hemacytometer.
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Bio-technique: HLA Typing
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Sunday, December 26, 2010. In 1975, Peter Doherty and Rolf Zinkernagel identified human leukocyte antigens (HLA). HLA are proteins found everywhere in the body except red blood cells. They are especially prevalent in white blood cells. Many types of HLA exist, often varying greatly from person to person. Doherty and Zinkernagel were awarded the 1996 Nobel Prize in Physiology or Medicine for this discovery. The accuracy of HLA typing increases with the rarity of a tested person's HLA. However, this pr...