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Self-Complementary AAV

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Stem Cell Media SFM. Self-Complementary AAV packaging services. Single-stranded DNA, which is about 4.7 k. And consists of two. Inverted terminal repeats (ITRs). At both ends and two open reading frames (ORFs). A rate-limiting step u. Which directly influences the efficiency of infection. In order to bypass this step, the coding region of wild-type AAV is modified to form. Upon transfection, the. Two complementary halves of scAAV will associate to form one double stranded DNA. Vg and many choices of.

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Protein Service

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Stem Cell Media SFM. The Protein Platform is equipped with advanced downstream facilities, such as fermentor, multi-functional bioreactor, Stansted high pressure homogenizer nano, GE QuixStand membrane separation system, GE AKTA systems, offering a variety of choices for gene expression, product purification, process optimization and amplification, which are applicable for different expression hosts and production scales. 1) Bacterial Expression System. The Protein Platform usually utilizes. The Protein ...

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BIOWIT TECHNOLOGIES

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Stem Cell Media SFM. Stem Cell Media SFM. Mesenchymal Stem Cell Medium-serum free(MSC-SFM). 2005-2011 BioWit Technologies Ltd.

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Adeno-associated virus

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Stem Cell Media SFM. Single-stranded DNA, which is about 4.7 k. And consists of two. Inverted terminal repeats (ITRs). At both ends and two open reading frames (ORFs). AAV is recognized as. A very attractive candidate. Viral vectors for gene therapy. Safety and the ability of ensuring a transgene stable and long-term expression in animals. There are 10 serotypes of AAV in Biowit Technologies. It is suggested to choose AAV according to different tissue types. And shRNA silencing knock down. Uscle, Liver, B.

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BIOWIT TECHNOLOGIES

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Stem Cell Media SFM. Your shopping cart is empty! 2005-2011 BioWit Technologies Ltd.

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Stable cell line

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Stem Cell Media SFM. Stable cell line generation. Generated many types of cell lines stably expressing foreign cDNA or shRNA, such as HEK293, 293T, CHO,. SiHa, 3D4/2, HL7702, SW480, HepG2, SGC-7901, MGC-803, HT-29, Saos-2, U-2os, PC12, SK-BR-3,. MCF-7, HMSC-ad, GIST-T1, etc. Two methods, Lentivirus infection and plasmids transfection, are used to transfer cDNA or shRNA. Neomycin. Or puromycin is the most widely used drug screening marker. CDNA or shRNA can be stably expressed for 15 generations;.

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cDNA expression

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Stem Cell Media SFM. Two or more genes can be cloned into one vector by using 2A lin. Er, IRES sequence,. Different promoters and fusion-protein techniques. Prokaryotic vectors, eukaryotic vectors and viral vectors (eg. lentivirus, adenovirus,. Diverse fluorescence reporter genes. GFP, eGFP, emGFP, ZsGreen, hrGFP. MCherry, DsRed-Express 2,. TdTomato, and YFP. MV, mCMV, EF1, PGK. SV40, CAG, Ubc and. His, Flag, GST, C-myc, HA, MBP etc. 2 weeks ( 1 kb) and 3. 2005-2011 BioWit Technologies Ltd.

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Non-integrated Lentivirus

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Stem Cell Media SFM. To develop efficient transient transfection methods, Biowit has also established a modified lentiviral vector system by. Inactivating the integrative elements of classical lentivruses. The. Lentivirus can efficiently infect a variety. Of mammalian cells like classical lentivirus whereas its viral DNA does not integrate into the host genome, thus it serve. As an excellent alternative for routine transient transfection while it can also avoid the integrative side effects of classical.

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Plasmid Construction

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Stem Cell Media SFM. Offers a wide-range of options for single or multiple shRNAs, cDNAs & microRNAs, as well. Eporter construct cloning. Choose from a variety of promoters,. Delivery systems including Lentivector. Adenoviurs vectors, AAV. Save yourself time and have team of experts. Build the custom constructs that you need. Custom-built services give you exactly what you need;. Provide us with cDNA template or just sequence information of gene-of-interest and let us know your experimental. 1 kb 2 kb.

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shRNA expression

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Stem Cell Media SFM. Single shRNA expression or. In the same vector target. Inducible vectors for shRNA expression also available;. Choose from many shRNA expression. Eukaryotic vectors, lentivirual vectors, adenovirus vector, and AAV. H1 or U6 promoter options. Diverse fluorescence reporter genes. GFP, eGFP, ZsGreen, tdTomato,. Provide us with the gene information and choose a vector. We will design shRNA sequence for you and we ensure. You at least 70% RNA silencing efficiency. Samples of shRNA vectors.