CANCER-BIOMARKERS.COM

ihc-antibodies.com

IHC antibodies: Choosing the Best Substrate

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Choosing the Best Substrate. Choosing the Best Detection Module for IHC. Immunohistochemistry (IHC) uses antibodies to selectively detect proteins in a cell or tissue. Detection relies on a secondary antibody to detect the first. This antibody will be conjugated to a reporter. The most common reporter molecules you will encounter in IHC are alkaline phosphatase and horseradish peroxidase. Both of these proteins react with target substrates to form a colored product. Furthermore, you can consider the sens...

ihc-antibodies.com

IHC antibodies: Optimization of IHC Experiments

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Optimization of IHC Experiments. Immunohistochemistry (IHC) is an extremely valuable technique used in a variety of different settings to determine protein location and amount. It is also a rather challenging technique that requires troubleshooting and a good understanding of principles. Here are some commonly-encountered concepts in IHC. No or Low Detection of Protein. There are two commonly-employed wash buffers used in IHC. These include tris-buffered saline and phosphate-buffered saline. Wash...Is th...

chip-antibodies.com

Chip Antibody: Antibody Selection for ChIP

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Antibody Selection for ChIP. How to Select the Best Antibody for ChIP assays. It must be selective. The antibody you choose needs to recognize your protein of interest alone. Once the assay is performed, you will not have a way to which protein the DNA stuck. Typically, researchers will take for granted that the antibody only purified the protein of interest, but. What if it also recognized a different DNA binding factor? 訂閱： 文章 (Atom). Antibody Selection for ChIP. Seqsite for Binding Site Analysis.

chip-antibodies.com

Chip Antibody: Seqsite for Binding Site Analysis

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Seqsite for Binding Site Analysis. Is used primarily to identify sites of DNA binding by proteins. Of course, it is imperative to optimize data analysis. This allows for the best interpretation of the information gained from ChIP. The method of ChIP-seq, a form of ChIP that combines the traditional purification with deep sequencing to detect the isolated DNA fragments, has been gaining in popularity over the more traditional ChIP-chip technique. 訂閱： 文章 (Atom). Antibody Selection for ChIP.

chip-antibodies.com

Chip Antibody: Troubleshooting ChIP

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As with any multi-step molecular biology method, chromatin immunoprecipitation (ChIP). Can show you some strange results and problems at times. You need to understand the many ways in which it can go wrong. This will allow you to properly troubleshoot the method. Here is a list of some of the common problems and how to address them. High Background That Is Also Found in Controls. Poor Resolution Coupled to High Background. Poor or problematic Amplification by PCR. 訂閱： 文章 (Atom). Watermark範本. 由 Blogger.

chip-antibodies.com

Chip Antibody: Comparing and Contrasting ChIP-seq and ChIP-chip

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Comparing and Contrasting ChIP-seq and ChIP-chip. There are many different flavors of chromatin immunoprecipitation (ChIP) experiments. Depending one what kinds of protein-DNA interactions you want to see. Advantages and Disadvantages of Each Method. The introduction of the microarray in ChIP-chip. 訂閱： 文章 (Atom). Antibody Selection for ChIP. Comparing and Contrasting ChIP-seq and ChIP-chip. Seqsite for Binding Site Analysis. Watermark範本. 由 Blogger.

stem-cell-marker.com

Stem Cell Markers: June 2015

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Stem cells are a diverse class of cells that share the ability to self-renew and to differentiate into multiple cell types. Monday, June 15, 2015. CD34 Stem Cell Antibodies from GeneTex. CD34 Antibodies Now Available. PROTEINS and PEPTIDES /. SERUM and REAGENTS /. Push Forward With CD34 Antibodies. GeneTex proudly offers a large selection of high-quality antibodies for mesenchymal stem cell and cancer research, including well-validated CD34 antibodies. Please see our highlighted products below:. CD29 / I...

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Stem Cell Markers: Neural Stem Cell Marker

http://www.stem-cell-marker.com/2013/09/Neural-Stem-Cell-Marker.html

Stem cells are a diverse class of cells that share the ability to self-renew and to differentiate into multiple cell types. Wednesday, September 25, 2013. Neural Stem Cell Marker. In the body, neural stem cells. Are found near blood vessels in the subventricular zone, the hippocampus. And the songbird higher vocal center. The cells in the ventricular zone release vascular endothelial growth factor (VEGF). In order to investigate the relationship between the neural stem cells. Were used as controls. Scien...