C.A.P.T.U.R.E. (CLARITY-based Activity and Projection Tracking upon Recombination) is a platform that combines rapid whole brain clearing, simple volumetric imaging, molecular genetics and computational neuroscience to study neural circuit in intact mammalian brains.
http://www.capture-clarity.org/
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CAPTURE | capture-clarity.org Reviews
https://capture-clarity.org
C.A.P.T.U.R.E. (CLARITY-based Activity and Projection Tracking upon Recombination) is a platform that combines rapid whole brain clearing, simple volumetric imaging, molecular genetics and computational neuroscience to study neural circuit in intact mammalian brains.
capture-clarity.org
CLARITY-based Tractography — CAPTURE
http://capture-clarity.org/clarity-based-tractography
CLARITY-based Activity and Projection Tracking upon Recombination. CLARITY-based tractography for projection tracking. Where p and r represent spatial locations, w is a Gaussian weighing function with standard deviation σg, S0 is a symmetric second-moment matrix derived from image intensity gradients:. Tractography and tractography visualization were performed using Diffusion Toolkit and TrackVis softwares (http:/ trackvis.org/). Matlab code can be download here. Five Hundred For the Win!
Optimized CLARITY — CAPTURE
http://capture-clarity.org/protocols
CLARITY-based Activity and Projection Tracking upon Recombination. Optimized CLARITY procedures for faster, simpler and affordable whole brain clearing and imaging. A hydrogel based on 1% acrylamide. 1% acrylamide, 0.0125% Bis, 4% PFA, 0.25% VA-044 initiator (w/v), in 1X PBS, as described in Tomer et al 2014) was used for all CLARITY preparations. Mice were transcardially perfused with ice-cold 4% PFA (not hydrogel yet). Containing 8% SDS for 6-12 hours to remove residual PFA and monomers. Brains cou...
Movies — CAPTURE
http://capture-clarity.org/movies
CLARITY-based Activity and Projection Tracking upon Recombination. Movie #1: 3D CLARITY image shows neural connections from the prefrontal cortex across an entire transparent mouse brain. 1080P and Full screen viewing recommended. Movie #2 : Long-Range Visualization and Tracing of NPAS4 /mPFC-NAc Projecting Cells. 1080P and Full screen viewing recommended. Five Hundred For the Win!
Image Gallery — CAPTURE
http://capture-clarity.org/image-gallery-1
CLARITY-based Activity and Projection Tracking upon Recombination. Duis mollis, est non commodo luctus, nisi erat porttitor ligula, eget lacinia odio sem nec elit. Maecenas faucibus mollis interdum. Nulla vitae elit libero, a pharetra augue. Five Hundred For the Win!
TRAP Analysis Pipeline — CAPTURE
http://capture-clarity.org/analysis-pipeline
CLARITY-based Activity and Projection Tracking upon Recombination. Computational pipeline for TRAP analysis. Data processing pipeline for image registration, cell detection, annotation and quantification. Codes for TRAP analysis can be download here. Duis mollis, est non commodo luctus, nisi erat porttitor ligula, eget lacinia odio sem nec elit. Maecenas faucibus mollis interdum. Nulla vitae elit libero, a pharetra augue. Five Hundred For the Win!
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SWIFT3D Resources
http://clarityresourcecenter.com/functional3D.html
RNA detection in CLARITY. RNA detection in CLARITY. Observing and controlling volumetric dynamics at cellular and near-cellular resolution. Light Field Microscopy for 3D functional neuroimaging. The invention of light field microscopy:. Levoy M, Ng R, Adams A, Footer M, and M Horowitz. SIGGRAPH. See the Stanford Light Field Microscope Project. Page for details and much additional work. Levoy M, Zhang Z, and I McDowell. J Microscopy. Grosenick L, Anderson T, and SJ Smith. IEEE ISBI. First public demonstra...
COLM Resources
http://clarityresourcecenter.com/COLM.html
RNA detection in CLARITY. RNA detection in CLARITY. SPED Light Sheet Microscopy: Fast Mapping of Biological System Structure and Function. Tomer R, Lovett-Barron M, Kauvar I, Andalman A, Burns VM, Sankaran S, Grosenick L, Broxton M, Yang S, and K Deisseroth. Cell. Intact-brain analyses reveal distinct information carried by SNc dopamine subcircuits. Lerner TN, Shilyansky C, Davidson TJ, Evans KE, Beier KT, Zalocusky KA, Crow AK, Malenka RC, Luo L, Tomer R, and K Deisseroth. Cell.
Fiber Photometry Resources
http://clarityresourcecenter.com/fiberphotometry.html
RNA detection in CLARITY. RNA detection in CLARITY. Fiber Photometry for imaging long-range projections. Hypothalamic control of male aggression-seeking behavior. Falkner AL, Grosenick L, Davidson TJ, Deisseroth K, and D Lin. Nature Neuroscience. Advanced Online (2016). Supplementary Information. Intact-brain analyses reveal distinct information carried by SNc dopamine subcircuits. Natural Neural Projection Dynamics Underlying Social Behavior. 157:1535–1551 (June 2014). *equal contributors.
CLARITY Resources
http://clarityresourcecenter.com/CLARITY.html
RNA detection in CLARITY. RNA detection in CLARITY. CLARITY News and Techniques. Comparing Tissue Transparency Methods for Intact Analyses. From Communication in Neural Circuits: Tools, Opportunities, and Challenges. Lerner TN, Ye L, and K Deisseroth. Cell. Transparent brains reveal effects of cocaine and fear (Nature). Microscopy: seeing through tissue (Nature Methods). CLARITY-optimized light-sheet microscopy (COLM). CLARITY training: The Deisseroth laboratory is now offering. Leading Edge Primer) 2016.
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Capture-Caroline-Costa's blog - Blog de Capture-Caroline-Costa - Skyrock.com
Coucuo,ce blog comportera des captures de videos de caroline costa :). 05/12/2010 at 2:56 PM. 09/12/2010 at 3:47 AM. Subscribe to my blog! Caroline Costa agée de 14ans est une étoile née le 9 Mai 1996,entourée de sa famille est de ses amis Caroline ateint les porte de la finale d'incroyable talent 2008,ou elle fini 2eme du classement sur 3000 personnes environ! Sur ce blog il y aura que des captures,quelques unes taguées et d'autres non! Mais aucunes photos privées! Mon deuxième blog sur Caroline est.
Capture-Caroline's blog - Capture video - Skyrock.com
Coucou , bein ici je vais poster des captures video de Caroline Costa =). Vous pouvez bien sur les prendre , Je ne dis pas nan MAIS dites le moi si vous les prenez s'il vous plait merci! 04/09/2010 at 5:50 AM. 23/01/2011 at 3:45 AM. Subscribe to my blog! Nan nan nan le but du blog n'est pas de Capturer Caroline . xD. Ce blog sera constituer de 1. Capture video sur bien evidemment Caroline Costa.Il y aura quelques montages. J'ai decider de couper le blog en deux! Une partie Capture et une partie Montage.
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Blog de Capture-cet-instant - " La mémoire ne filme pas, la mémoire photographie " Milan Kundera - Skyrock.com
Mot de passe :. J'ai oublié mon mot de passe. La mémoire ne filme pas, la mémoire photographie Milan Kundera. Mise à jour :. Abonne-toi à mon blog! Ce blog n'a pas encore d'articles. Poster sur mon blog.
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CAPTURE
CLARITY-based Activity and Projection Tracking upon Recombination. CLARITY-based Activity and Projection Tracking upon Recombination). A platform that combines rapid whole brain clearing, simple volumetric imaging, molecular genetics and computational analysis to study circuit structure and functions in intact mammalian brains. Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences. CAPTURE for TRAP (activity-based cell body detection and analysis).
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