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Casil.io – Next-generation CRISPR/Cas9 platform

Without permanently changing genetic sequences. The problems and previous solutions. Our solution to both the multiplexing and multimerization problems – Casilio. We have constructed an RNA-aptamer based approach called Casilio to improve both multiplexing and multimerization capabilities to CRISPRzyme toolkits [19]. Casilio is a hybrid system combining CRISPR/d Cas. 9 and Pum ilio. The components of Casilio (using Sp-dCas9). Cas9 with D10A and H840A mutations. List of vectors expressing sgRNA-PBS. One-s...

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Casil.io – Next-generation CRISPR/Cas9 platform | casil.io Reviews

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Without permanently changing genetic sequences. The problems and previous solutions. Our solution to both the multiplexing and multimerization problems – Casilio. We have constructed an RNA-aptamer based approach called Casilio to improve both multiplexing and multimerization capabilities to CRISPRzyme toolkits [19]. Casilio is a hybrid system combining CRISPR/d Cas. 9 and Pum ilio. The components of Casilio (using Sp-dCas9). Cas9 with D10A and H840A mutations. List of vectors expressing sgRNA-PBS. One-s...

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1 introduction

2 papers

3 module registry

4 sgrna pbs

5 press

6 updates

7 developers

8 pbs2hexid

9 navigation

10 crisprzymes

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Casil.io – Next-generation CRISPR/Cas9 platform | casil.io Reviews

https://casil.io

Without permanently changing genetic sequences. The problems and previous solutions. Our solution to both the multiplexing and multimerization problems – Casilio. We have constructed an RNA-aptamer based approach called Casilio to improve both multiplexing and multimerization capabilities to CRISPRzyme toolkits [19]. Casilio is a hybrid system combining CRISPR/d Cas. 9 and Pum ilio. The components of Casilio (using Sp-dCas9). Cas9 with D10A and H840A mutations. List of vectors expressing sgRNA-PBS. One-s...

INTERNAL PAGES

casil.io

1

Updates – Casil.io

http://casil.io/updates

New web apperance: Casilio in Chinese! EBiotrade (生物通), a website in China, has written about Casilio:. Http:/ www.ebiotrade.com/newsf/2016-1/2016118120734767.htm. Other appearances in China:. Http:/ www.v2gg.com/geti/danweiguanfang/20160117/56853.html. January 18, 2016. Press release at JAX. Joyce Peterson wrote a press release for Casilio. Check it out! Https:/ www.jax.org/news-and-insights/2016/january/casilio-upgrades-crispr. January 15, 2016. Our paper is online at. We are also doing press releases!

2

Press – Casil.io

http://casil.io/press

Joyce Peterson (JAX). Casilio Gives CRISPR-Cas An Upgrade. Jan 15, 2016. Https:/ www.jax.org/news-and-insights/2016/january/casilio-upgrades-crispr. Epigenie. Casilio: a CRISPR/Cas9 and Pumilio Hybrid for Epigenome Editing. Jan 24, 2016. EBiotrade (生物通). 中科院王皓毅博士开发新型通用CRISPR-Cas9-Pumilio系统. Jan 19, 2016. Http:/ www.ebiotrade.com/newsf/2016-1/2016118120734767.htm. 生物极客 Casilio:定制化sgRNA激活和成像. Jan 16, 2016. Http:/ www.v2gg.com/geti/danweiguanfang/20160117/56853.html. Theme by: Theme Horse.

3

New web apperance: Casilio in Chinese! – Casil.io

http://casil.io/new-web-apperance-casilio-in-chinese

New web apperance: Casilio in Chinese! EBiotrade (生物通), a website in China, has written about Casilio:. Http:/ www.ebiotrade.com/newsf/2016-1/2016118120734767.htm. Other appearances in China:. Http:/ www.v2gg.com/geti/danweiguanfang/20160117/56853.html. January 18, 2016. Press release at JAX. Press release at JAX. January 15, 2016. Joyce Peterson wrote a press release for Casilio. Check it out! January 15, 2016. Our paper is online at Cell Research! Theme by: Theme Horse.

4

Online! – Casil.io

http://casil.io/online

Our paper is online at. Http:/ www.nature.com/cr/journal/vaop/ncurrent/full/cr20163a.html. We are also doing press releases! More info and protocols! January 15, 2016. Press release at JAX →. New web apperance: Casilio in Chinese! January 18, 2016. EBiotrade (生物通), a website in China, has written about Casilio:. Press release at JAX. January 15, 2016. Joyce Peterson wrote a press release for Casilio. Check it out! Theme by: Theme Horse.

5

sgRNA-PBS – Casil.io

http://casil.io/sgrna-pbs

These are published and unpublished sgRNA-PBS expression vectors for use with Casilio modules. Many are available on Addgene. Please contact us. To update the list if you constructed new sgRNA-PBS expression vectors. PBS Sequence (in DNA alphabet). Theme by: Theme Horse.

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cheng.bio

Publications | Albert Cheng Lab

http://cheng.bio/pub

Albert Cheng, PhD. The Jackson Laboratory for Genomic Medicine. All types ( 32 ). Cancer Metastasis ( 3 ). Cheng Lab ( 1 ). CRISPR/Cas TALEN ( 8 ). Diseases ( 2 ). Epigenetics ( 10 ). Patents ( 1 ). PhDEra ( 28 ). PostDocEra ( 2 ). Pre-PhD ( 1 ). Representative ( 7 ). RNA Splicing RBPs ( 5 ). Stem Cells Reprogramming ( 13 ). Synthetic Biology Genome Engineering ( 5 ). Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling. Synthetic Biology Genome Engineering. Loci simul...

cheng.bio

LabPics | Albert Cheng Lab

http://cheng.bio/labpics

Albert Cheng, PhD. The Jackson Laboratory for Genomic Medicine. A souvenir to commemorate the grand opening of JAX-GM. The Jackson Laboratory for Genomic Medicine.

cheng.bio

Bio/informatics | Albert Cheng Lab

http://cheng.bio/informatics

Albert Cheng, PhD. The Jackson Laboratory for Genomic Medicine. This page is underconstruction. Information may be inaccurate or incomplete. WHAT WE ARE DOING NOW. Currently, we don’t have specific projects in this section but we are open to collaboration on analysis of RNA-seq data, specifically, identification of alternative splicing. We deposit and share tools developed along our research at github https:/ github.com/albertwcheng.

cheng.bio

Bio/engineering | Albert Cheng Lab

http://cheng.bio/engineering

Albert Cheng, PhD. The Jackson Laboratory for Genomic Medicine. This page is underconstruction. Information may be inaccurate or incomplete. The Cheng Lab develops technologies based on artificial DNA and RNA binding proteins for genome editing, epigenome editing and transcriptome editing. For DNA binding proteins, the main one used in the lab is CRISPR/Cas9. For RNA binding proteins, the main one is Pumilio. Pumilio proteins are . Improving precise genome editing. Developing sensors for DNA and RNA.

cheng.bio

Team | Albert Cheng Lab

http://cheng.bio/sketches

Albert Cheng, PhD. The Jackson Laboratory for Genomic Medicine. Albert Cheng, PhD. Aziz Taghbalout, PhD. Lab Manager/Research Assistant III.

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Casil.io – Next-generation CRISPR/Cas9 platform

Without permanently changing genetic sequences. The problems and previous solutions. Our solution to both the multiplexing and multimerization problems – Casilio. We have constructed an RNA-aptamer based approach called Casilio to improve both multiplexing and multimerization capabilities to CRISPRzyme toolkits [19]. Casilio is a hybrid system combining CRISPR/d Cas. 9 and Pum ilio. The components of Casilio (using Sp-dCas9). Cas9 with D10A and H840A mutations. List of vectors expressing sgRNA-PBS. One-s...

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