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cellcycler.org - Cycler

Welcome to cellcycler.org This website has been created by the Pelkmans Lab  (University of Zurich), the Liberali Lab (FMI Basel) and the Pe'er Lab (Columbia University), to provide the experimental...

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cellcycler.org - Cycler | cellcycler.org Reviews

https://cellcycler.org

Welcome to cellcycler.org This website has been created by the Pelkmans Lab  (University of Zurich), the Liberali Lab (FMI Basel) and the Pe&#039;er Lab (Columbia University), to provide the experimental...

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1

Image Acquisition - cellcycler.org

http://www.cellcycler.org/downloads.html

Step 1: Sample Preparation. Step 2: Image Processing and Quantification. Step 3: Build a Cell Cycle Trajetory. In this section you can find all downloadable material used in the 3 steps to your personal Cell Cycle Trajectory. Everything (Step1, Step2, Step3 and Manual). Cycler Step1 ClickIt EdU Protocol.xlsx. Image Processing and Quantification. Cp1 custommodule cycler.zip. Cp1 customsubfunction cycler.zip. Build a Cell Cycler Trajectory. Proudly powered by Weebly.

2

Image Acquisition - cellcycler.org

http://www.cellcycler.org/step-3-build-a-cell-cycle-trajetory.html

Step 1: Sample Preparation. Step 2: Image Processing and Quantification. Step 3: Build a Cell Cycle Trajetory. Step 3: Build a Cell Cycle Trajectory. This section explains how to import your single-cell data into Cycler, process it and construct a Cell Cycle Trajectory. Download Cycler (see Download section or below) and add it to your MatLab path. Type "cycler" in to your MatLab command line to open the Cycler GUI. Intensity OrigBlue Nuclei 1 IntegratedIntensity. Texture OrigFarRed Nuclei 12 InfoMeas1.

3

About Us - cellcycler.org

http://www.cellcycler.org/about-us.html

Step 1: Sample Preparation. Step 2: Image Processing and Quantification. Step 3: Build a Cell Cycle Trajetory. Cycler has been developed by joining forces from the Liberali Lab, Pelkmans Lab and Pe'er Lab. The Liberali Lab studies c. For general questions and collaborations concerning this work. Does quantitative cell biology by combining experiments with computation, using large-scale approaches and advanced imaging. For general questions and collaborations concerning this work. Proudly powered by Weebly.

4

Image Acquisition - cellcycler.org

http://www.cellcycler.org/step-1-sample-preparation.html

Step 1: Sample Preparation. Step 2: Image Processing and Quantification. Step 3: Build a Cell Cycle Trajetory. Step 1: Sample Preparation. This section explains in a step-by-step protocol how to prepare your sample for image acquisition to later construct a Cell Cycle Trajectory with Cycler. Grow your cells to desired confluency. Add EdU at a final concentration of 200µM to your cells and incubate for 15min at 37°C and 5% CO2. Fix cells at 4% Paraformaldehyde for 15min. Thoroughly wash cells with PBS.

5

Image Acquisition - cellcycler.org

http://www.cellcycler.org/step-2-image-processing--quantification.html

Step 1: Sample Preparation. Step 2: Image Processing and Quantification. Step 3: Build a Cell Cycle Trajetory. Step 2: Image Processing and Quantification. This section explains step-by-step the image analysis protocol in CellProfiler 1, which results in a MatLab file that can be imported into Cycler for classification of G1, S, G2 and M cells, and the construction of a Cell Cycle Trajectory. Copy all images of the experiment you want to analyze with Cycler into one folder (e.g. CyclerExp01). To detect n...

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Step 1: Sample Preparation. Step 2: Image Processing and Quantification. Step 3: Build a Cell Cycle Trajetory. Welcome to cellcycler.org. This website has been created by the Pelkmans Lab. University of Zurich), the Liberali Lab. FMI Basel) and the Pe'er Lab. Columbia University), to provide the experimental protocols and computational tools to build a Cell Cycle Trajectory (CCT) using Cycler. To construct a Cell Cycle Trajectory and study your cell activity or protein of interest along the cell cycle, c.

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