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CoLBeRT: Optogenetic tool for neuroscience

An optogenetic technique for neuroscience that uses lasers to manipulate neurocircuits in moving animals. CoLBeRT is a new technique for neuroscience developed by the Samuel Lab. At Harvard University's Center For Brain Science. That allows researchers to commandeer the nervous system of a moving animal without wires or electrodes. The system has been used thus far on the microscopic nematode. See the recent articles about the CoLBeRT system in Science News.

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CoLBeRT: Optogenetic tool for neuroscience | colbert.physics.harvard.edu Reviews

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An optogenetic technique for neuroscience that uses lasers to manipulate neurocircuits in moving animals. CoLBeRT is a new technique for neuroscience developed by the Samuel Lab. At Harvard University's Center For Brain Science. That allows researchers to commandeer the nervous system of a moving animal without wires or electrodes. The system has been used thus far on the microscopic nematode. See the recent articles about the CoLBeRT system in Science News.

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CoLBeRT: Optogenetic tool for neuroscience | colbert.physics.harvard.edu Reviews

https://colbert.physics.harvard.edu

An optogenetic technique for neuroscience that uses lasers to manipulate neurocircuits in moving animals. CoLBeRT is a new technique for neuroscience developed by the Samuel Lab. At Harvard University's Center For Brain Science. That allows researchers to commandeer the nervous system of a moving animal without wires or electrodes. The system has been used thus far on the microscopic nematode. See the recent articles about the CoLBeRT system in Science News.

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colbert.physics.harvard.edu

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CoLBeRT: Code

http://colbert.physics.harvard.edu/code.php

All of the software written for the CoLBeRT system is freely available under the open source GNU General Public License. Visit the Samuel Lab page. The software to track a moving worm and generate illumination patterns targeted at specific neurons or muscle is called MindControl. MindControl was written in C by Andrew Leifer using the open source OpenCV. Computer vision library. The source code for mindcontrol is available on GitHub. Package and run the software using included video of a swimming worm.

2

CoLBeRT: Press

http://colbert.physics.harvard.edu/press.php

Steve Bradt, Light touch: Using beam of light, scientists commandeer worm's nervous system. Julie R. Barzilay, Harvard Scientists Control Minds of Worms. In the popular press. Laura Sanders, Making a worm do more than squirm. David Castelvecchi, "Single Worm Neurons Remotely Controlled with Lasers". Sam Sheffer, Harvard University controls worm wtih laser, we wait for choreagraphed dance moves (video). Andrew Moseman, Zapping Worm Brains With Lasers Reveals the Duty of Each Neuron.

3

CoLBeRT: Videos

http://colbert.physics.harvard.edu/videos.php

Inactivating a worm's muscles. The nematode C. elegans. Shown here expresses the gene for Channelrhodopsin in all of its muscle cells. When green laser light shines on the worm, its muscles relax and the worm paralyzes. When laser light shines everywhere else outside of the worm's boundary, the worm does not respond. This worm expresses Channelrhodopsin in its HSN neurons ( Pegl-6: ChR2: GFP. Disrupting locomotion by inhibiting anterior muscles. Disrupting locomotion by inhibiting motor neurons. When gre...

4

CoLBeRT: Images

http://colbert.physics.harvard.edu/images.php

The CoLBeRT system can aquire an image of the worm , identify target neurons and direct its digital micromirror device to shine light on those targets in less than 20ms closed-loop. The worm sits in a chamber on the microscope (center). The optics are located in the black box on the optics bench (right) . Computer software (left) controls the entire apparatus. Is written in C and uses the open source OpenCV. Computer vision library. The worm is only 1mm long. Software segments the worm into 100 sectors.

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CoLBeRT: Optogenetic tool for neuroscience

http://colbert.physics.harvard.edu/index.php

An optogenetic technique for neuroscience that uses lasers to manipulate neurocircuits in moving animals. CoLBeRT is a new technique for neuroscience developed by the Samuel Lab. At Harvard University's Center For Brain Science. That allows researchers to commandeer the nervous system of a moving animal without wires or electrodes. The system has been used thus far on the microscopic nematode. See the recent articles about the CoLBeRT system in Science News.

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Seeing is Believing – Harvard University Brain Tour

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Seeing is Believing… And A First Step to Understanding! Since Van Leeuwenhoek introduced the first real microscope in the 17. Century, successive enhancements in microscopy have enabled visualization of ever tinier processes in the natural world. Working in the late 19. And a summary of his life and work is here. Zeiss Stand I laboratory compound microscope. Collection of Historical Scientific Instruments (1126a) 1885 (Image Zeiss). Is one of the most innovative biological imaging centers in the world&#4...

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colbert.physics.harvard.edu

CoLBeRT: Optogenetic tool for neuroscience

An optogenetic technique for neuroscience that uses lasers to manipulate neurocircuits in moving animals. CoLBeRT is a new technique for neuroscience developed by the Samuel Lab. At Harvard University's Center For Brain Science. That allows researchers to commandeer the nervous system of a moving animal without wires or electrodes. The system has been used thus far on the microscopic nematode. See the recent articles about the CoLBeRT system in Science News.

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