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Home » Centre for Protein Research

The Centre for Protein Research (CPR) is a facility that is supported by the Departments of Biochemistry and Microbiology and Immunology, and is available to all researchers within the University of Otago. The CPR combines both a service facility and a user laboratory that supports research projects largely within the University of Otago. Analyses for external institutes are also provided. A TripleTOF 5600 mass spectrometer has recently been installed and provides advanced capabilities in.

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Home » Centre for Protein Research | cpr.otago.ac.nz Reviews

https://cpr.otago.ac.nz

The Centre for Protein Research (CPR) is a facility that is supported by the Departments of Biochemistry and Microbiology and Immunology, and is available to all researchers within the University of Otago. The CPR combines both a service facility and a user laboratory that supports research projects largely within the University of Otago. Analyses for external institutes are also provided. A TripleTOF 5600 mass spectrometer has recently been installed and provides advanced capabilities in.

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1

Instrumentation » Centre for Protein Research

http://cpr.otago.ac.nz/instrumentation

LTQ-Orbitrap hybrid mass spectrometer. ABI 4800 MALDI tandem time-of-flight mass spectrometer. We are performing small and large scale 2-D gel electrophoresis (IEF coupled with SDS-PAGE) for protein separation and quantification of relative protein abundances. 2-D PAGE is particularly suitable for differentially displaying the protein complements (up to ca. 1500 protein spots per sample) of two or more related samples such as disease vs healthy, time course experiments etc. more ». The Probot workstation...

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TripleTOF 5600+ » Centre for Protein Research

http://cpr.otago.ac.nz/instrumentation/tripletof-5600

Raquo; TripleTOF 5600. The TripleTOF 5600 system is a hybrid mass spectrometer that combines high performance quadrupole (Qq) mass spectrometry with high resolution Time-of-Flight (TOF) data acquisition and therefore provides a comprehensive platform for discovery and targeted proteomics as well as metabolomics and lipidomics. The wide mass range of the TOF-analyser (up to m/z 40,000) also facilitates intact protein mass measurements. And high resolution MRM.

3

Request for analysis » Centre for Protein Research

http://cpr.otago.ac.nz/request-for-analysis

Please download and complete the Request for Analysis form (*.doc or *.pdf) and send either a digitally signed electronic version of the pdf form via email or a signed hard copy together with your sample(s) to the Centre for Protein Research. If you are not sure which procedure is required for analysis of your sample leave the required analysis section blank and we can discuss with you the details of analysis. Download Request for Analysis form as.

4

Staff » Centre for Protein Research

http://cpr.otago.ac.nz/people

Tel: 64 3 479-7867. FAX: 64 3 479-7866. E-mail: torsten.kleffmann@otago.ac.nz. Tel: 64 3 479-7542. FAX: 64 3 479-7866. E-mail: diana.carne@otago.ac.nz. Tel: 64 3 479-7542. FAX: 64 3 479-7866. E-mail: abhishek.kumar@otago.ac.nz. Tel: 64 3 479-7871. FAX: 64 3 479-7866. E-mail: catherine.day@otago.ac.nz. Tel: 64 3 479-7722. FAX: 64 3 479-7866. E-mail: gregory.cook@otago.ac.nz. University of Otago Powered by SilverStripe Open Source CMS.

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Protocols » Centre for Protein Research

http://cpr.otago.ac.nz/workflows

Protein identification from gel bands/spots. Protein bands/spots of interest are excised from the gel and digested in-gel with a site specific protease such as trypsin. The tryptic peptides are eluted from the gel matrix and subjected to mass spectrometric analysis. Single protein bands/spots are usually analysed by MALDI tandem Time-of-Flight mass spectrometry. more ». High throughput protein identification/proteomics. 2-Dimensional Polyacrylamide Gel Electrophoresis (2-D PAGE). In 2-D PAGE protein comp...

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Home » Centre for Protein Research

The Centre for Protein Research (CPR) is a facility that is supported by the Departments of Biochemistry and Microbiology and Immunology, and is available to all researchers within the University of Otago. The CPR combines both a service facility and a user laboratory that supports research projects largely within the University of Otago. Analyses for external institutes are also provided. A TripleTOF 5600 mass spectrometer has recently been installed and provides advanced capabilities in.

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