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Cy0 - A new method in Real-Time PCR quantification - home

The Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. The method is based on the fit of Richards' equation to real-time PCR data by nonlinear regression in order to obtain the best fit estimators of reaction parameters. Subsequently, these parameters were used to calculate the Cy0 value that minimizes the dependence of its value on PCR kinetic. Our results demonstrated that when PCR efficiency was slightly decreased, d

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Cy0 - A new method in Real-Time PCR quantification - home | cy0method.org Reviews
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The Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. The method is based on the fit of Richards' equation to real-time PCR data by nonlinear regression in order to obtain the best fit estimators of reaction parameters. Subsequently, these parameters were used to calculate the Cy0 value that minimizes the dependence of its value on PCR kinetic. Our results demonstrated that when PCR efficiency was slightly decreased, d
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Cy0 - A new method in Real-Time PCR quantification - home | cy0method.org Reviews

https://cy0method.org

The Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. The method is based on the fit of Richards' equation to real-time PCR data by nonlinear regression in order to obtain the best fit estimators of reaction parameters. Subsequently, these parameters were used to calculate the Cy0 value that minimizes the dependence of its value on PCR kinetic. Our results demonstrated that when PCR efficiency was slightly decreased, d

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Cy0 - A new method in Real-Time PCR quantification - A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition,Michele Guescini*�1,2 , Davide Sisti�3 , Marco BL Rocchi3

https://www.cy0method.org/publications/new-real-time-PCR-method.php

Cy0 - A new qPCR quantification method. Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition. Real-time PCR analysis is a sensitive DNA quantification technique that has recently gained considerable attention in biotechnology, microbiology and molecular diagnostics. Although, the cycle-threshold ( Ct. Metho...

2

Cy0 - A new method in Real-Time PCR quantification - Shape based kinetic outlier detection in real-time PCR

https://www.cy0method.org/publications/shape-based-kinetic-outlier-detection-in-RT-PCR.php

Cy0 - A new qPCR quantification method. Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. Shape Based Kinetic Outlier Detection. Our results demonstrated, for the first time. That outlier detection can be based on amplification shape instead of PCR efficiency. SOD represents an improvement in real-time PCR analysis because it decreases the variance of data thus increasing the reliability of quantification. Furthermo...

3

Cy0 - A new method in Real-Time PCR quantification - Quantify with Cy0 on-line

https://www.cy0method.org/gestione-accessi/quantify_online.php

Cy0 - A new qPCR quantification method. Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. Try our method on line. This site is born in order allow to our visitors to test our quantification method. And if required, our new patent. By uploading their raw PCR data files. And getting quantification results within 1 day. Just login ☺ →. Enter E-mail. Further required to log in! 2010 Cy0Team - University of Urbino.

4

Cy0 - A new method in Real-Time PCR quantification - Quantify with Cy0 on-line

https://www.cy0method.org//gestione-accessi/quantify_online.php

Cy0 - A new qPCR quantification method. Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. Try our method on line. This site is born in order allow to our visitors to test our quantification method. And if required, our new patent. By uploading their raw PCR data files. And getting quantification results within 1 day. Just login ☺ →. Enter E-mail. Further required to log in! 2010 Cy0Team - University of Urbino.

5

Cy0 - A new method in Real-Time PCR quantification - Cys Patent

https://www.cy0method.org/patent/index.php

Cy0 - A new qPCR quantification method. Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. No need to estimate efficiency through assumption of equal efficiency between unknowns and standard curve. Get quantification results also in very sub-optimal amplification condition. Extremely easy to implement in Real Time PCR instruments software. Cool Title Right Here. Click - this is a button. Cy0method.org - 2012. This si...

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Cy0 - A new method in Real-Time PCR quantification - home

Cy0 - A new qPCR quantification method. Cy0 is a new method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. Since the main work. Inspiring this site, we got many feedbacks and tens of uploads which allowed us to expand the view on the result already achieved:. Cy0 is still the first method in Real-time PCR analysis that does not require the assumption of equal efficiency between unknowns and standard curve. In this sense, every globa...

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