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Is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (. That allows for dual views (. Of the sample while mounted on an inverted (. Microscope (i.e. the SPIM objectives are upright). The diSPIM was co-developed by the lab of Hari Shroff. At NIH/NIBIB and Applied Scientific Instrumentation. ASI) SPIM is also referred to as light sheet fluorescence microscopy. Or LSFM because it uses a sheet or plane of light to illuminate the sample perpendicular to the imaging direction.

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start - diSPIM Wiki | dispim.org Reviews

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Is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (. That allows for dual views (. Of the sample while mounted on an inverted (. Microscope (i.e. the SPIM objectives are upright). The diSPIM was co-developed by the lab of Hari Shroff. At NIH/NIBIB and Applied Scientific Instrumentation. ASI) SPIM is also referred to as light sheet fluorescence microscopy. Or LSFM because it uses a sheet or plane of light to illuminate the sample perpendicular to the imaging direction.

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start - diSPIM Wiki | dispim.org Reviews

https://dispim.org

Is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (. That allows for dual views (. Of the sample while mounted on an inverted (. Microscope (i.e. the SPIM objectives are upright). The diSPIM was co-developed by the lab of Hari Shroff. At NIH/NIBIB and Applied Scientific Instrumentation. ASI) SPIM is also referred to as light sheet fluorescence microscopy. Or LSFM because it uses a sheet or plane of light to illuminate the sample perpendicular to the imaging direction.

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lists.dispim.org

dispim-users Info Page

Dispim-users - diSPIM users. This list is for discussion of diSPIM and iSPIM microscopes. These are modular light sheet microscopes based on hardware from ASI mounted on top of an inverted microscope. diSPIM systems image the sample from the two views alternatively, allowing a 3D reconstruction with isotropic resolution. diSPIM systems are available from ASI and multiple system integrators. All questions/discussion/suggestions concerning diSPIM systems can be carried out here. If you choose not to enter ...

INTERNAL PAGES

dispim.org

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media:video_tutorials - diSPIM Wiki

http://dispim.org/media/video_tutorials

New Technologies for High Spatial and Temporal Resolution Imaging by Hari Shroff. DiSPIM technique overview by Hari Shroff. DiSPIM microscope assembly (ASI). DiSPIM dis-assembly/packaging for upgrade (ASI). July 2015, not yet final but helpful). DiSPIM assembly at NCBS. Sept 2014; the students assembled from components whereas it is delivered to customers mostly assembled). Comparison light sheet methods. Last modified: 2016/01/26 18:48 by. CC Attribution-Share Alike 4.0 International.

2

docs:user_list - diSPIM Wiki

http://dispim.org/docs/user_list

Here is a list of labs with working diSPIM systems. If your lab is missing please add it! University of Heidelberg, Intelligent Imaging Group (PI Christian Conrad). NIH, lab of Hari Shroff (4 experimental systems). University of Washington, Waterston Lab. The Norwegian Radium Hospital, Stenmark lab http:/ www.ous-research.no/stenmark/. More than 30 diSPIM systems are in the field, so this list is incomplete. Comparison light sheet methods. Last modified: 2016/05/19 17:19 by.

3

docs:system_integrators - diSPIM Wiki

http://dispim.org/docs/system_integrators

DiSPIM systems can be purchased from the following companies:. Applied Scientific Instrumentation (ASI). Originator of microscope hardware regardless of the integrator. Offers integration services, but no on-site support. Demos available in Eugene Oregon USA. Contributor to Micro-Manager diSPIM plugin. Offers integration and on-site support. Uses Micro-Manager diSPIM plugin. Various other system integrators around the world, including. Comparison light sheet methods. Last modified: 2016/07/19 22:44 by.

4

docs:manual - diSPIM Wiki

http://dispim.org/docs/manual

Attach the SPIM assembly to the microscope frame. Care of piezo objective movers. Programmable Logic Card (or TTL) Card Connections. Light Path A Component Connections. Light Path B Component Connections. Laser and Fiber Installation. Install filters, Filter cubes and Mirrors. How to adjust the mirror cubes. Before Running the Plugin. Setting the 3D ROI. Tab-specific joystick, sheet, and camera controls. Imaging center and calibration display. Position display and sheet control. Set the Stage Limits.

5

media:publications - diSPIM Wiki

http://dispim.org/media/publications

Dual-view plane illumination microscopy for rapid and spatially isotropic imaging. Nat Protoc. 2014 Nov;9(11):2555-73. doi: 10.1038/nprot.2014.172. Detailed protocol for diSPIM, some details different (and much easier) with more modern systems. Two views on light sheets. Nat Biotechnol. 2013 Nov;31(11):992-3. doi: 10.1038/nbt.2739. Overview of Spatially isotropic four-dimensional imaging with dual-view plane illumination microscopy . Guide to light-sheet microscopy for adventurous biologists. Myosin-II-m...

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start - diSPIM Wiki

Is a flexible and easy-to-use implementation of Selective Plane Illumination Microscopy (. That allows for dual views (. Of the sample while mounted on an inverted (. Microscope (i.e. the SPIM objectives are upright). The diSPIM was co-developed by the lab of Hari Shroff. At NIH/NIBIB and Applied Scientific Instrumentation. ASI) SPIM is also referred to as light sheet fluorescence microscopy. Or LSFM because it uses a sheet or plane of light to illuminate the sample perpendicular to the imaging direction.

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