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Cell Photometrics laboratory

The Cell Photometrics Laboratory develops fully engineered instruments and new methods for biomedical research and clinical applications. Recent projects include a commercial automated 768-lane Sanger sequencing instrument, a 384-channel parallel imaging microfluidic cytometer, a commercial van-portable forensic DNA analyser, an important new method for highly multiplexed quantitative (qPCR) expression analysis, and a new deep-ultraviolet method for weighing the intracellular nucleic acid and protein...

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Cell Photometrics laboratory | djehrlich.com Reviews

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The Cell Photometrics Laboratory develops fully engineered instruments and new methods for biomedical research and clinical applications. Recent projects include a commercial automated 768-lane Sanger sequencing instrument, a 384-channel parallel imaging microfluidic cytometer, a commercial van-portable forensic DNA analyser, an important new method for highly multiplexed quantitative (qPCR) expression analysis, and a new deep-ultraviolet method for weighing the intracellular nucleic acid and protein...

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Cell Photometrics laboratory | djehrlich.com Reviews

https://djehrlich.com

The Cell Photometrics Laboratory develops fully engineered instruments and new methods for biomedical research and clinical applications. Recent projects include a commercial automated 768-lane Sanger sequencing instrument, a 384-channel parallel imaging microfluidic cytometer, a commercial van-portable forensic DNA analyser, an important new method for highly multiplexed quantitative (qPCR) expression analysis, and a new deep-ultraviolet method for weighing the intracellular nucleic acid and protein...

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djehrlich.com

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384-Lane Parallel Microfluidic Cytometer

http://djehrlich.com/index.php/384-lane-parallel-microfluidic-cytometer

384-Lane Parallel Microfluidic Cytometer. Under NIH funding we are now developing a novel 384-channel parallel microfluidic cytometer (PMC) that is capable of high-content (image-based) and rare-cell assays. This system will have more than ten-times the sample throughput of microscope-based imaging systems and may help to move cell-based assays to new applications in drug discovery, genomics, and cancer research. Detail of the confocal scanner used as a detector on the PMC. Lab on a Chip 9, 305-310, 2009.

2

GeneTrack: Mobile DNA Forensics

http://djehrlich.com/index.php/genetrack-mobile-dna-forensics

GeneTrack: Mobile DNA Forensics. Under funding from the National Institute of Justice, we developed the first van-portable microfluidic DNA finger-printing instrument. GeneTrack is currently a commercial product in use for rapid (e.g., crime-scene) forensics. Goedecke N, McKenna B, El-Difrway S, Gismondi E, Swenson A, Carey L, Matsudaira P, and Ehrlich DJ, Microdevice DNA Forensics by the Simple Tandem Repeat Method. J Chroma A, 1111, 206-213 (2006). 768-Lane Microfluidic DNA Sequencing System.

3

Reaction Mapped PCR

http://djehrlich.com/index.php/reaction-mapped-pcr

Ueberfeld J, McKenna B, Rubin-Berjerano I, Verstrepen, K, and Ehrlich DJ; Reaction-Mapped. Quantitative Multiplexed PCR on a Microfluidic Device. Analytical Chemistry, 80, 7430-7436 (2008). Ueberfeld J and Ehrlich DJ; Scaling of Nucleic Acid Assays on Microelectrophoresis Array Devices: High-. Dynamic Range Multi-gene Readout from less than 10 Transcripts. Electrophoresis 2009, 30, 2090 2099. 768-Lane Microfluidic DNA Sequencing System. 384-Lane Parallel Microfluidic Cytometer.

4

768-Lane Microfluidic DNA Sequencing System

http://djehrlich.com/index.php/768-lane-microfluidic-dna-sequencing-system

768-Lane Microfluidic DNA Sequencing System. Under NIH funding we develped a 768-lane Sanger sequencing machine that has been commercialized by Shimadzu Biotechnology. The BioMEMS-768 operates with large format microfabricated glass plates and is capable of long read (1000 base-pair) de novo DNA sequencing sequencing. El-Difrawy SA, Lam R, Aborn JH, Novotny M, Gismondi EA, Matsudaira P, McKenna BK, O’Neil T, Streechon P and Ehrlich DJ: High throughput system for DNA sequencing.

5

Nucleic acid and protein mass mapping by live-cell deep-UV microscopy

http://djehrlich.com/index.php/nucleic-acid-and-protein-mass-mapping-by-live-cell-deep-uv-microscopy

Nucleic acid and protein mass mapping by live-cell deep-UV microscopy. Quantification of nucleic acid and protein content in fixed and live cells. Background subtracted, raw optical density images. Nucleic acid and protein mass maps compared to nucleic acid stains. Distribution of protein and nucleic acid mass compared to nucleic acid dyes. Mass maps can reveal details, comparable to staining, at 100x magnification. Cytometry A, 79A, 920-932, 2011. Nature Methods 4(7); 567-569, 2007.

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Cell Photometrics laboratory

The Cell Photometrics Laboratory develops fully engineered instruments and new methods for biomedical research and clinical applications. Recent projects include a commercial automated 768-lane Sanger sequencing instrument, a 384-channel parallel imaging microfluidic cytometer, a commercial van-portable forensic DNA analyser, an important new method for highly multiplexed quantitative (qPCR) expression analysis, and a new deep-ultraviolet method for weighing the intracellular nucleic acid and protein...

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