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Thursday, February 14, 2013. Test of Fast ID kit. Fresh vs 7 day-old T. cacao. Leaves to be tested using FastID Genomic DNA extraction kit. Type of leaves: Most are fully expanded (some with green, some with brown petioles) except where unavailable. Some noticeably softer than others. Leaf bags labelled DT (dry tissue-1week) or FT (fresh tissue). Potentially should pulse to briefly settle debris before transferring sample from H-tube to exclude bead in centrifugation step. Washed at 12K rpm for 5 min - GW.

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Thursday, February 14, 2013. Test of Fast ID kit. Fresh vs 7 day-old T. cacao. Leaves to be tested using FastID Genomic DNA extraction kit. Type of leaves: Most are fully expanded (some with green, some with brown petioles) except where unavailable. Some noticeably softer than others. Leaf bags labelled DT (dry tissue-1week) or FT (fresh tissue). Potentially should pulse to briefly settle debris before transferring sample from H-tube to exclude bead in centrifugation step. Washed at 12K rpm for 5 min - GW.

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DNA Lab | dna-lab.blogspot.com Reviews

https://dna-lab.blogspot.com

Thursday, February 14, 2013. Test of Fast ID kit. Fresh vs 7 day-old T. cacao. Leaves to be tested using FastID Genomic DNA extraction kit. Type of leaves: Most are fully expanded (some with green, some with brown petioles) except where unavailable. Some noticeably softer than others. Leaf bags labelled DT (dry tissue-1week) or FT (fresh tissue). Potentially should pulse to briefly settle debris before transferring sample from H-tube to exclude bead in centrifugation step. Washed at 12K rpm for 5 min - GW.

INTERNAL PAGES

dna-lab.blogspot.com

1

DNA Lab: January 2006

http://dna-lab.blogspot.com/2006_01_01_archive.html

Saturday, January 21, 2006. 70 mL CIAA per 100 extracts. 100 mL Isopropanol per 100 extracts. 100 mL Ethanol per 100 extracts. D-squared note: Overnight pptn of DNA ok. Pellets unaffected. EDTA Used in March 2002: Disodium dihydrate. Gel quantitation: Dryloading works well only if yield is good. TAQ Buffer Magnesium Chloride 50U/mL: 0.1 mL per reaction. Group A: 27/28; 45A/45B; 53/54B; 132/133. Group D: 35/36; 43/44. Ladder (Lambda) 114ng/mL: 25 mL per gel. Sigma Urea dissolves in.

2

DNA Lab: DNA Extraction etc

http://dna-lab.blogspot.com/2006/03/dna-extraction-etc.html

Tuesday, March 07, 2006. Sheet BF 37-72 last fp#: 3336. Leaf tissue for extraction prepared on previous day. Gave MB list of items to order. Prepared tips and tubes for autoclaving. Subscribe to: Post Comments (Atom). Prep, pptn etc. Simple template. Template images by gaffera.

3

DNA Lab: February 2013

http://dna-lab.blogspot.com/2013_02_01_archive.html

Thursday, February 14, 2013. Test of Fast ID kit. Fresh vs 7 day-old T. cacao. Leaves to be tested using FastID Genomic DNA extraction kit. Type of leaves: Most are fully expanded (some with green, some with brown petioles) except where unavailable. Some noticeably softer than others. Leaf bags labelled DT (dry tissue-1week) or FT (fresh tissue). Potentially should pulse to briefly settle debris before transferring sample from H-tube to exclude bead in centrifugation step. Washed at 12K rpm for 5 min - GW.

4

DNA Lab: PCR and Electrophoresis

http://dna-lab.blogspot.com/2006/01/pcr-and-electrophoresis.html

Saturday, January 21, 2006. TAQ Buffer Magnesium Chloride 50U/mL: 0.1 mL per reaction. Group A: 27/28; 45A/45B; 53/54B; 132/133. Group D: 35/36; 43/44. Ladder (Lambda) 114ng/mL: 25 mL per gel. Sigma Urea dissolves in. Subscribe to: Post Comments (Atom). Simple template. Template images by gaffera.

5

DNA Lab: About

http://dna-lab.blogspot.com/2006/02/about.html

Friday, February 10, 2006. A CRU DNA Lab chronicle of observations, notes, protocols, recipes etc and other general information which maybe useful to archive. Subscribe to: Post Comments (Atom). Simple template. Template images by gaffera.

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Thursday, February 14, 2013. Test of Fast ID kit. Fresh vs 7 day-old T. cacao. Leaves to be tested using FastID Genomic DNA extraction kit. Type of leaves: Most are fully expanded (some with green, some with brown petioles) except where unavailable. Some noticeably softer than others. Leaf bags labelled DT (dry tissue-1week) or FT (fresh tissue). Potentially should pulse to briefly settle debris before transferring sample from H-tube to exclude bead in centrifugation step. Washed at 12K rpm for 5 min - GW.

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