DOT-IT-SPOT-IT.COM

Dot it Spot it

Dot it Spot it. Dot-it-Spot-it Total Protein Assay. This sensitive and rapid total protein assay works well with both detergents and reducing substances (like DTT)! One big area for interest is the quantification of proteins in cerebrospinal fluid (CSF), after depletion of several abundant proteins. The main amount of protein is required for the MS analysis, and only minute protein amounts (nanograms! Can be used for estimation of total protein. Maria Lönnberg at Uppsala University, Sweden. Dot-it-Spot-i...

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Maplestone AB

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SWEDEN

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Dot it Spot it | dot-it-spot-it.com Reviews

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Dot it Spot it. Dot-it-Spot-it Total Protein Assay. This sensitive and rapid total protein assay works well with both detergents and reducing substances (like DTT)! One big area for interest is the quantification of proteins in cerebrospinal fluid (CSF), after depletion of several abundant proteins. The main amount of protein is required for the MS analysis, and only minute protein amounts (nanograms! Can be used for estimation of total protein. Maria Lönnberg at Uppsala University, Sweden. Dot-it-Spot-i...

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1 method

2 results

3 applications

4 order

5 play

6 pause

7 analysis

8 dot it spot it is rapid

9 dot it spot it is easy to use

10 tillbaka upp

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Dot it Spot it | dot-it-spot-it.com Reviews

https://dot-it-spot-it.com

Dot it Spot it. Dot-it-Spot-it Total Protein Assay. This sensitive and rapid total protein assay works well with both detergents and reducing substances (like DTT)! One big area for interest is the quantification of proteins in cerebrospinal fluid (CSF), after depletion of several abundant proteins. The main amount of protein is required for the MS analysis, and only minute protein amounts (nanograms! Can be used for estimation of total protein. Maria Lönnberg at Uppsala University, Sweden. Dot-it-Spot-i...

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dot-it-spot-it.com

1

Applications « Dot it Spot it

http://dot-it-spot-it.com/applications

Dot it Spot it. General use of Dot-it-Spot-it Protein Assay. For all protein assays you will need to select a reference protein for use as calibration curve. The reference protein has to be diluted in the same buffer as you have your samples in. For protein concentrations below 0.1 mg/mL there will be a substantial loss of protein to surfaces like tube walls. Detergents are commonly added to reduce the loss of protein. Calibrate with a protein or protein mixture that corresponds to your samples.

2

Company « Dot it Spot it

http://dot-it-spot-it.com/company

Dot it Spot it. Situated in the small village Knivsta in the middle of Sweden, is a family owned company having decades of experience from research in the biotechnology and diagnostic area. Link to LinkedIn for information about the founder and main owner Maria Lönnberg. The Biotech part of Maplestone is focused on the novel and unique product Dot-it-Spot-it, an assay for total protein concentration estimation. Reg Office: Knivsta, Sweden.

3

Order « Dot it Spot it

http://dot-it-spot-it.com/order

Dot it Spot it. Dot-it-Spot-it Total Protein Assay kit for 480 estimations. 1 kit: Price 358 EUR. 2-5 kit: Price for each kit 289 EUR. 6 kit or more: Price for each kit 241 EUR. For Dot-it-Spot-it Total Protein Assay kit). Price: 44 EUR ,. When delivered together with the test kit. 8 reservoirs with clips. Dispensing template, 2 clips, light source. The following information is needed to process your order:. VAT-number for European Union (EU) countries. Import charges are not included in the price.

4

Results « Dot it Spot it

http://dot-it-spot-it.com/results

Dot it Spot it. Results fot HSA 0.16-10 ug/ml = 0.2-10 ng. Sample: HSA, Sigma A8763 diluted in 1% SDS, 20 mM TRIS pH 7,5, 0,15 M NaCl, 0.02 % NaN3. A Semiquantitative detection by eye. B Quantitative detection with scanner, Epson Perfection V600 Photo, using Epson Scan software. Microgram/mL or nanogram HSA. Studies that have been using Dot-it-Spot-it Protein Assay. 1 Intracellular concentration of the tyrosine kinase inhibitor imatinib in gastrointestinal stromal tumor cells. Anticancer Drugs. 4 Lipodis...

5

Method « Dot it Spot it

http://dot-it-spot-it.com/method

Dot it Spot it. Step by step instruction for Dot-it-Spot-it protein assay, 01.02.2015. Detection recommendation for Dot-it-Spot-it protein assay, 01.06.2015. Template for calculation 02.04.2016. Apply 1 uL of sample and dry. 1 mL Detection solution. 1 mL Washing solution. Scan with an image scanner. Quantify the blackness intensity. Along the 11 cm Detection sheet. You can apply 24 dots with 1-μL aliquots of protein sample. The kit contains the black Detection solution,. And 20 Detections sheets,. March ...

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Dot it Spot it

Dot it Spot it. Dot-it-Spot-it Total Protein Assay. This sensitive and rapid total protein assay works well with both detergents and reducing substances (like DTT)! One big area for interest is the quantification of proteins in cerebrospinal fluid (CSF), after depletion of several abundant proteins. The main amount of protein is required for the MS analysis, and only minute protein amounts (nanograms! Can be used for estimation of total protein. Maria Lönnberg at Uppsala University, Sweden. Dot-it-Spot-i...

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