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Frayne Consultants

Biotechnology for the chemical modification of RNA & DNA in vivo -creates nuclease resistant nucleic acids in all types of cells.

http://www.frayneconsultants.com/

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CONTACTS AT FRAYNECONSULTANTS.COM

ELIZABETH FRAYNE

1249 S. DI●●●●●●●●●● BLVD. 125

DIAM●●●● BAR , CA, 91765

US

1.90●●●●7415
EF●●●●●@VERIZON.NET

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ELIZABETH FRAYNE

1249 S. DI●●●●●●●●●● BLVD. 125

DIAM●●●● BAR , CA, 91765

US

1.90●●●●7415
EF●●●●●@VERIZON.NET

View this contact

ELIZABETH FRAYNE

1249 S. DI●●●●●●●●●● BLVD. 125

DIAM●●●● BAR , CA, 91765

US

1.90●●●●7415
EF●●●●●@VERIZON.NET

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Biotechnology for the chemical modification of RNA & DNA in vivo -creates nuclease resistant nucleic acids in all types of cells.
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frayne consultants,vosyn,technology,dna stabilizer,rna stabilizer,protein synthesis modulator,non toxic mutagenesis,about us,vivosyn biotechnology,chemically,focus areas,protein,3 cell secretion,mutagenesis,vivosyn,dna vaccines,protein synthesis
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Biotechnology for the chemical modification of RNA & DNA in vivo -creates nuclease resistant nucleic acids in all types of cells.

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1

Phosphate analogue - Frayne Consultants

http://www.frayneconsultants.com/products

Phosphate Analgoues to Synthesize Modified RNA and DNA Nucleic Acids In Vivo. Stabilize mRNA in cell and modulate protein expression. Phosphate analogue monosubstituted . Phos-1S. Phosphate analogue disubstituted . Phos-2S. Limited License is available for non-DNA applications in research and/or manufacturing of non-nucleic acid products. Subscribe Here for New Updates. Subscribing allows you to get site updates. Your email address will be kept private. Chemically modified DNA plasmid.

2

VivoSyn - About Us

http://www.frayneconsultants.com/about-us

Phosphate Analgoues to Synthesize Modified RNA and DNA Nucleic Acids In Vivo. Stabilize mRNA in cell and modulate protein expression. Greater Los Angeles Area. 1249 S. Diamond Bar, Diamond CA. Subscribe Here for New Updates. Subscribing allows you to get site updates. Your email address will be kept private. 15068 Rosecrans Ave. #162 , La Mirada California United States 90638. Chemically modified DNA plasmid.

3

Non toxic mutagenesis - Frayne Consultants

http://www.frayneconsultants.com/mutagenesis

Phosphate Analgoues to Synthesize Modified RNA and DNA Nucleic Acids In Vivo. Stabilize mRNA in cell and modulate protein expression. Non-Toxic Mutagenesis in Bacteria. Useful for mutating recombinant DNA phage clones and/or libraries using multiple rounds of growth and selection of viable phage. Bacterial cells can be grown under selective conditions when screening for phenotypes with an increased or decreased quantitative index. Subscribe Here for New Updates. Chemically modified DNA plasmid.

4

Enhance protein expression - Frayne Consultants

http://www.frayneconsultants.com/protein

Phosphate Analgoues to Synthesize Modified RNA and DNA Nucleic Acids In Vivo. Stabilize mRNA in cell and modulate protein expression. 1) Shift the distribution of proteins in cells in favor of less abundant and/or heterologous proteins. 2) Increase the protein synthesis of heterologous proteins and/or native proteins normally expressed at lower levels. Increase total protein secretion in eukaryotic cells such as yeast 2-4 fold several fold. 4) Identify regulatory networks. 5) Promotes differentiated state.

5

Chemically modified DNA plasmids - Frayne Consultants

http://www.frayneconsultants.com/dna

Phosphate Analgoues to Synthesize Modified RNA and DNA Nucleic Acids In Vivo. Stabilize mRNA in cell and modulate protein expression. DNase resistant DNA, plasmids, produced in vivo. 1 Digestion of Goldfish DNA. Digestion of M13 ss Phage DNA. 3 Efficiency of Incorporation Into plasmid DNA. Step 1) Denature DNA sample. Step 2) Digest sample with S1 nuclease. To individual nucleotides (leaves 5 prime phosphate). Step 3) Digest nucleotides with Alkaline phosphatase. Step 4) Measure free ortho phosphate.

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Phosphate Analgoues for the Synthesize Modified RNA and DNA In Vivo. RNA stabilization in cells can also modulate protein expression. Phosphate analogues, via a universal mechanism of action, can be used in cells and cell systems to create. Modified nucleic acids with nuclease resistant backbones. In vivo RNA stabilization of all cellular RNA with accumulation and dramatic elevation of total amount of mRNA per cell. And/or heterologous proteins) a. The expense of naturally more abundant protein.

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