cagef.utoronto.ca
Genomics | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/services/next-generation-genomics
We provide both Next Generation Sequencing with the Illumina system and high throughput Sanger sequencing with the 48 capillary AB system. Along with standard sequencing, we also perform CAGEF-developed techniques:. Identifies the causative lesion in Arabidopsis EMS generated mutants. Provides highly informative concatenated short sequence reads of variable regions of the 16S rRNA gene for microbiome research. Illumina NextSeq500 Desktop Sequencer v2. Illumina MiSeq Personal Sequencer v2.
cagef.utoronto.ca
Transcriptomics | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/services/transcriptomics
Our transcriptomics platform consists of both traditional microarray and Illumina RNA-Seq services. Affymetrix GeneChip Transcriptome Profiling System. GeneChips currently available in house:. Arabidopsis genome tiling array 1.0R. Can also interrogate user provided:. Genome tiling arrays for ChIP-chip studies. Custom arrays designed for resequencing. Illumina Solexa Genome Analyzer IIx with Paired End capabilities. Next generation high-throughput genome analyzer can be applied to:. Thakur S, Guttman DS.
cagef.utoronto.ca
Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2 | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/quantitative-interactor-screening-with-next-generation-sequencing-qis-seq-identifies-arabidopsis-thaliana-mlo2-as-a-target-of-the-pseudomonas-syringae-type-iii-effector-hopz2-2
Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2. Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2. Lewis JD, Wan J, Ford R, Gong Y, Fung P, Nahal H, Wang PW, Desveaux D, Guttman DS. BMC Genomics 2012 ;13():8. Thakur S, Guttman DS. The auxin response fa...
cagef.utoronto.ca
Services | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/services
CAGEF maintains a variety of state-of-the-art equipment allowing us to provide a range of services for your research needs. All services include an initial consultation and help with study design. A De-Novo Genome Analysis Pipeline (DeNoGAP) for large-scale comparative prokaryotic genomics studies. Thakur S, Guttman DS. Genomic screens identify a new phytobacterial microbe-associated molecular pattern and the cognate Arabidopsis receptor-like kinase that mediates its immune elicitation.
cagef.utoronto.ca
Next-generation mapping of Arabidopsis genes | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/next-generation-mapping-of-arabidopsis-genes
Next-generation mapping of Arabidopsis genes. Next-generation mapping of Arabidopsis genes. Austin RS, Vidaurre D, Stamatiou G, Breit R, Provart NJ, Bonetta D, Zhang J, Fung P, Gong Y, Wang PW, McCourt P, Guttman DS. Plant J. 2011 Aug;67(4):715-25. Population. This method, called Next Generation Mapping (NGM), uses a chastity statistic to quantify the relative contribution of the parental mutant and mapping lines to each SNP in the pooled F? Lines and a single channel of Illumina Genome Analyzer data....
cagef.utoronto.ca
The embryonic leaf identity gene FUSCA3 regulates vegetative phase transitions by negatively modulating ethylene-regulated gene expression in Arabidopsis | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/the-embryonic-leaf-identity-gene-fusca3-regulates-vegetative-phase-transitions-by-negatively-modulating-ethylene-regulated-gene-expression-in-arabidopsis-2
The embryonic leaf identity gene FUSCA3 regulates vegetative phase transitions by negatively modulating ethylene-regulated gene expression in Arabidopsis. The embryonic leaf identity gene FUSCA3 regulates vegetative phase transitions by negatively modulating ethylene-regulated gene expression in Arabidopsis. Lumba S, Tsuchiya Y, Delmas F, Hezky J, Provart NJ, Shi Lu Q, McCourt P, Gazzarrini S. BMC Biol. 2012 ;10():8. Thakur S, Guttman DS. Genomic screens identify a new phytobacterial microbe-associated m...
cagef.utoronto.ca
Bio-Analytic Resource | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/services/bio-analytic-resource
The BAR) provides user-friendly web-based tools for working with functional genomics and other data. Most are designed with the plant (mainly Arabidopsis) researcher in mind, but a couple of them can be useful to the wider research community. A De-Novo Genome Analysis Pipeline (DeNoGAP) for large-scale comparative prokaryotic genomics studies. Thakur S, Guttman DS. Mott GA, Thakur S, Smakowska E, Wang PW, Belkhadir Y, Desveaux D, Guttman DS. Krogan NT, Marcos D, Weiner AI, Berleth T.
cagef.utoronto.ca
Flow Cytometry | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/services/flow-cytometry
Our facility has both cell sorting and cell counting abilities. For sorting and / or counting of fluorescently-labeled cells. Can be used to perform:. Traditional flow cytometric applications. A De-Novo Genome Analysis Pipeline (DeNoGAP) for large-scale comparative prokaryotic genomics studies. Thakur S, Guttman DS. Genomic screens identify a new phytobacterial microbe-associated molecular pattern and the cognate Arabidopsis receptor-like kinase that mediates its immune elicitation.
cagef.utoronto.ca
About | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/about
CAGEF is closely allied with the departments of Cell and Systems Biology. And Ecology and Evolutionary Biology. At the University of Toronto. And a proud supporter and sponsor of the Collaborative Graduate Program in Genome Biology and Bioinformatics. Next Generation Mapping harnesses the power of Next Generation Sequencing technology to efficiently map point mutations, identifying the causative lesion of phenotypically identifiable mutations. Austin et al., 2011. Quantitative Interactor Screen Sequencing.
cagef.utoronto.ca
Graduate Education | CAGEF Centre for the Analysis of Genome Evolution & Function
http://www.cagef.utoronto.ca/education-outreach/graduate-education
METHODS IN GENOMICS AND PROTEOMICS. Lecturer: Dr. P. Wang. A De-Novo Genome Analysis Pipeline (DeNoGAP) for large-scale comparative prokaryotic genomics studies. Thakur S, Guttman DS. Genomic screens identify a new phytobacterial microbe-associated molecular pattern and the cognate Arabidopsis receptor-like kinase that mediates its immune elicitation. Mott GA, Thakur S, Smakowska E, Wang PW, Belkhadir Y, Desveaux D, Guttman DS. Krogan NT, Marcos D, Weiner AI, Berleth T. CAGEF is closely allied with:.
