international.neb.com
Protocol for Dephosphorylation of 5'-ends of DNA using CIP (NEB #M0290) | New England Biolabs
http://international.neb.com/protocols/1/01/01/protocol-for-dephosphorylating-with-cip
Skip to main content. Protocol for Dephosphorylation of 5´-ends of DNA using CIP (NEB #M0290). Protocols.io also provides an interactive version of this protocol. Where you can discover and share optimizations with the research community. Prepare a 20 μl reaction as follows:. 1 pmol of DNA ends*. To 20 μl*. Incubate at 37°C for 30 minutes. Purify DNA by gel purification, spin-column or. Note: 1 pmol of DNA ends is about 1 μg of a 3 kb plasmid. Scale larger reaction volumes proportionally. Email: info....
international.neb.com
Competitor Cross-Reference Tool | New England Biolabs
http://international.neb.com/tools-and-resources/interactive-tools/competitor-cross-reference-tool
Skip to main content. Use this tool to select another company's competent cell product and find out which NEB strain is compatible. Choose either the product name or catalog number from the available selection, and this tool will identify the recommended NEB product and its advantages. A link to the product page where you can also order the product is provided. Please select a product name. 96 pack Gold Competent Cells. BL21-Gold (DE3) Competent Cells. BL21-Gold (DE3)pLysS Competent Cells. General Inquir...
international.neb.com
Decapping eukaryotic mRNA with RppH (NEB #M0356) | New England Biolabs
http://international.neb.com/protocols/2015/01/14/decapping-eukaryotic-mrna-with-rpph-neb-m0356
Skip to main content. Decapping eukaryotic mRNA with RppH (NEB #M0356). Nuclease-free water: 40 μl. 10X NEB Thermopol buffer: 5 μl (1X= 20mM Tris-HCl, 10mM (NH. 10mM KCl, 2mM MgSO. 01% Triton X-100, pH 8.8@25°C). RNA up to 500 ng of RNA: 5 μl. RppH (5,000 units/ml): 1 μl per 100 ng of RNA up 5 μl for 500 ng. If using less than 5 μl RppH make volume of reaction up to 50 μl with water. Mix, pipette up and down a minimum of six times. Incubate for one hour at 37°C. 50 µl reaction. 5 ng – 500 ng. Technique: ...
international.neb.com
Gibson Assembly® Master Mix – Assembly (E2611) | New England Biolabs
http://international.neb.com/protocols/2012/09/25/gibson-assembly-master-mix-assembly
Skip to main content. Gibson Assembly Master Mix – Assembly (E2611). Protocols.io also provides an interactive version of this protocol. Where you can discover and share optimizations with the research community. Or using the following formula:. Pmols = (weight in ng) x 1,000 / (base pairs x 650 daltons). 50 ng of 5000 bp dsDNA is about 0.015 pmols. 50 ng of 500 bp dsDNA is about 0.15 pmols. Set up the following reaction on ice:. Recommended Amount of Fragments Used for Assembly. Total Amount of Fragments.
