gmf.fas.harvard.edu

Genome Modification Facility, Harvard University

Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. The Genome Modification Facility (GMF) provides transgenic, gene targeting, and other services to investigators of Harvard University and its affiliated institutions, as well as to investigators within the US and abroad. The GMF performs microinjections of DNA into fertilized embryos to generate transgenic mice. DNA transfection into ES cells for the creation of recombinant ES cell clones. ES Cell Gene Targeting.

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Genome Modification Facility, Harvard University | gmf.fas.harvard.edu Reviews

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Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. The Genome Modification Facility (GMF) provides transgenic, gene targeting, and other services to investigators of Harvard University and its affiliated institutions, as well as to investigators within the US and abroad. The GMF performs microinjections of DNA into fertilized embryos to generate transgenic mice. DNA transfection into ES cells for the creation of recombinant ES cell clones. ES Cell Gene Targeting.

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8 embryo cryopreservation

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Genome Modification Facility, Harvard University | gmf.fas.harvard.edu Reviews

https://gmf.fas.harvard.edu

Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. The Genome Modification Facility (GMF) provides transgenic, gene targeting, and other services to investigators of Harvard University and its affiliated institutions, as well as to investigators within the US and abroad. The GMF performs microinjections of DNA into fertilized embryos to generate transgenic mice. DNA transfection into ES cells for the creation of recombinant ES cell clones. ES Cell Gene Targeting.

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gmf.fas.harvard.edu

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Services and Fees | Genome Modification Facility, Harvard University

https://gmf.fas.harvard.edu/pages/services-and-fees

Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. Embryonic Stem Cell Gene Targeting. ES or iPS Cell Line Services. ES or iPS Cell Microinjection - including KOMP/EUCOMM ES cell clones. TALEN or CRISPR Microinjection. International Knockout Mouse Consortium (KOMP/EUCOMM).

2

Embryonic Stem Cell Gene Targeting | Genome Modification Facility, Harvard University

https://gmf.fas.harvard.edu/pages/embryonic-stem-cell-gene-targeting

Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. Embryonic Stem Cell Gene Targeting. Non-Harvard Academic and Non-Profit. ES cell gene targeting service includes:. Electroporation of ES cells, drug-selection, colony picking, expansion of clones and production of replica plates for genotyping by investigator. ES cell lines available: V6.5 (129 x B6 F1 hybrid), JM8A3 or JM8A3N1 (B6N from KOMP/EUCOMM). We provide 2 x 96 clones for genotyping. The GMF reserves the r...

3

Quarantine Policies | Genome Modification Facility, Harvard University

https://gmf.fas.harvard.edu/node/9911

Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. The Office of Animal Resources at Harvard University and the Center for Comparative Medicine at MGH. Have agreed on the quarantine policies for GMF mice between these two institutions. They are as follows:. Express Quarantine at MGH. Mice produced in the GMF for MGH investigators will be subject to Express Quarantine at MGH. This process consists of the following steps:. Beth Israel Deaconess Hospital.

4

ES or iPS Cell Line Services | Genome Modification Facility, Harvard University

https://gmf.fas.harvard.edu/es-or-ips-cell-line-services

Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. ES or iPS Cell Line Services. Non-Harvard Academic and Non-Profit. Validation of human or mouse iPS cells by teratoma formation. Including implantation of one cell line into 3 NOD-Scid mice, housing and daily monitoring of mice for up to 11 weeks, removing teratoma tumor and fixation of tissue based on instruction from customer or iPS Core, and forwarding fixed tissue to investigator. Derivation of ES Cell line:.

5

Cryopreservation | Genome Modification Facility, Harvard University

https://gmf.fas.harvard.edu/genome-modification-facility/pages/cryopreservation

Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. Non-Harvard Academic and Non-Profit. Sperm Cryopreservation, including quality control (QC) test to 2-cell stage. Recovery of live pups following sperm cryopreservation and QC. Cryopreserve 200-300 embryos, in 10 straws. Including QC testing to generate live pups. Embryo Cryopreservation: from IVF (Speed Cryo). Embryos and Sperm Cryopreservation: Economical Solution for Freezing. Cryostorage Fee (per year). We req...

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cbs.unix.fas.harvard.edu

Core Facilities | Center for Brain Science

http://cbs.unix.fas.harvard.edu/science/core-facilities

Center for Brain Science. Our core facilities provide researchers with unique, state-of-the-art equipment. These cores are dynamic: we plan for them to evolve as new tools and techniques are developed into equipment for general use. They are also places to explore new technologies and invent new devices. The Neuroimaging facility has a 3-Tesla magnetic resonance imaging scanner for non-invasive human brain imaging. Our aim is to:. This core facility provides:. Laser scanning microscopes with motorized st...

cbs.fas.harvard.edu

Core Facilities | Center for Brain Science

http://cbs.fas.harvard.edu/science/core-facilities

Center for Brain Science. Our core facilities provide researchers with unique, state-of-the-art equipment. These cores are dynamic: we plan for them to evolve as new tools and techniques are developed into equipment for general use. They are also places to explore new technologies and invent new devices. The Neuroimaging facility has a 3-Tesla magnetic resonance imaging scanner for non-invasive human brain imaging. Our aim is to:. This core facility provides:. Laser scanning microscopes with motorized st...

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Genome Modification Facility, Harvard University

Skip to main content. Genome Modification Facility, Harvard University. How to Get Started. The Genome Modification Facility (GMF) provides transgenic, gene targeting, and other services to investigators of Harvard University and its affiliated institutions, as well as to investigators within the US and abroad. The GMF performs microinjections of DNA into fertilized embryos to generate transgenic mice. DNA transfection into ES cells for the creation of recombinant ES cell clones. ES Cell Gene Targeting.

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