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上海其明信息技术有限公司 – 基因数据处理 GCBI 测序 基因芯片 | gminix.com Reviews
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1、 小RNA数据 来源于 基因芯片或PCR芯片. 2、 LncRNA mRNA数据 来源于 基因芯片、RNA-Seq数据。 确定LncRNA靶向调控的显著性基因功能 根据GSEA算法 Gene SetsEnrichment Analysis 计算靶基因在信号通路中的权重，评估靶基因分布的最关键信号通路/基因功能. 确定LncRNA靶向调控的显著性基因功能 根据GSEA算法 Gene Sets Enrichment Analysis 计算靶基因在信号通路中的权重，评估靶基因分布的最关键信号通路/基因功能. STC(Series Test of Cluster). STC(Series Test of Cluster). 以Affymetrix GeneChip miRNA 4.0 Array差异筛选为例进行结果讲解。 1挑选差异倍数较大的microRNA，例如Flod Change 2 or 1.5. 2挑选显著性水平较高的microRNA，例如P 0.05 or 0.01. 1挑选差异倍数较大的基因，例如Flod Change 2 or1.5.
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GCBI，Gene-Cloud of Biotechnology Information. Http:/ www.gcbi.com.cn. Sample，输入关键词搜索得到相关样本在GEO数据库的信息，包括Sample ID、Sample title、Sample type、特点(组织、性别、疾病阶段、周期)等信息，对不同实验室的同类实验数据对比分析、信息整合等，可进一步挖掘其中所包含的生物信息。 小试牛刀，未来GCBI实验室将承载更大的科研任务，除了加入更多的芯片检测平台，还将引入真正的大数据处理能力 测序数据分析，帮助科研人员从 得到数据 到 得到结果 都可以全部在线完成，引领生物大数据的真正1.0时代。 版权所有 上海其明信息技术有限公司 版权所有2010-2015 沪ICP备1021149 Designed by Wanhu.
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GeneChip Human Transcriptome Array 2.0(HTA2.0)芯片是新一代全转录组芯片，该芯片设计了近700万条探针，可以检测超过28万条全长转录本，包括超过24万条编码转录本和超过4万条非编码转录本。 参考文献 Pin Wang et al. Science. Cancer Discovery 发表的研究成果表明，HTA 2.0与RNA-seq相比，能够获得更多的选择性拼接信息，而且全部通过了RT-PCR这一黄金标准的验证。 Simon J. Furney等使用芯片等多种工具寻找葡萄膜黑色素瘤 uveal melanoma 的致病基因。 剪接体的功能是将前体mRNA剪接成成熟的mRNA，而 Splicer factor 3b的功能正是让前体mRNA锚定于剪接体上。 参考文献 Simon J. Furney et al. Cancer Discovery. PLoS One 的一份于结肠癌相关的展示了HTA2.0芯片在基因表达差异化研究中的应用。 参考文献 Sahlberg SH et al. PLoS ONE. Data Sheet -TAC Software.
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上海其明信息技术有限公司 – 基因数据处理 GCBI 测序 基因芯片
我们发表过 科学 、 自然 、 细胞 等全球最顶级科学期刊 拥有4项基因计算方法专利，9项基因信息分析软件著作权;协助完成 110篇SCI. 我们帮助作者用HTA 2.0 transcriptome microarray assay检测了mono分化至DC过程中(7天)lncRNA的表达变化，在124个显著变化的lncRNA中，有24个被诱导。 更多项目介绍请移步 科研项目技术案例 和 生物IT项目案例.
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