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greenfluorescentblog | A blog on fluorescent microscopy in biological research

A blog on fluorescent microscopy in biological research

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greenfluorescentblog | A blog on fluorescent microscopy in biological research | greenfluorescentblog.wordpress.com Reviews
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greenfluorescentblog | A blog on fluorescent microscopy in biological research | greenfluorescentblog.wordpress.com Reviews

https://greenfluorescentblog.wordpress.com

A blog on fluorescent microscopy in biological research

INTERNAL PAGES

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1

How to… | greenfluorescentblog

https://greenfluorescentblog.wordpress.com/how-to

A blog on fluorescent microscopy in biological research. Calculate pixel size on images. Choose the right fluorescent protein ( part 1. Calculate numerical aperture and resolution. FISH for RNA ( basics. Basics of spot analysis. Click to email (Opens in new window). Click to print (Opens in new window). Click to share on Google (Opens in new window). Share on Facebook (Opens in new window). Click to share on Twitter (Opens in new window). Click to share on LinkedIn (Opens in new window). Fill in your det...

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Corrigendum | greenfluorescentblog

https://greenfluorescentblog.wordpress.com/2015/08/04/corrigendum

A blog on fluorescent microscopy in biological research. Tracking membranes by imaging – mCLING and surface glycans. Using Thresholds to Measure and Quantify Cells in Image J →. August 4, 2015. The paper then goes through several rounds of changes, following reviewers’ comments, editor’s comments, final proof-reading etc…. The accepted manuscript (MS) should be “perfect”. But mistakes can happen. Mistakes did. Happen for three of my recent papers. For my Cell paper. Then, we published a paper in PLOS One.

3

greenfluorescentblog | A blog on fluorescent microscopy in biological research | Page 2

https://greenfluorescentblog.wordpress.com/page/2

A blog on fluorescent microscopy in biological research. Newer posts →. ASCB15 – part 3. January 4, 2016. I ended part 2 Monday night. It was an exciting day with many excellent talks, but the best talk (mine, of course! Was due the next day. Continue reading →. ASCB15 – part 2. December 31, 2015. I ended Part 1. After the morning session on pushing the boundaries of imaging. After this session, I attended my first discussion table . Continue reading →. December 17, 2015. Continue reading →. I have sever...

4

Tracking membranes by imaging – mCLING and surface glycans | greenfluorescentblog

https://greenfluorescentblog.wordpress.com/2015/04/14/tracking-membranes-by-imaging-mcling-and-surface-glycans

A blog on fluorescent microscopy in biological research. Visualizing translation: insert TRICK pun here. Tracking membranes by imaging – mCLING and surface glycans. April 14, 2015. Living cells exhibit many types of membranes which participate in most biological precesses, one way or another. Imaging membranes is usually acheived by two types of reagents: chemical dyes or fluorescent proteins that are targeted to the membrane itself or inside an organelle. Tetramethylrosamine, and Mitotracker. Bind the p...

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Science and Wine | Science and wine… | Page 2

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Science and wine…. 2014 Books Read Challenge! SDL Monthly Book Club. Newer posts →. The Annual Meeting for the Society for Neuroscience – #SFN2013. November 6, 2013. It is that time of year again- the clocks have been set back, most deciduous trees in New England have shed their leaves, and my Siberian huskies their coats (mostly in the house), and autumn has brought us cool, crisp evenings signaling winters impending chill. Continue reading →. Quick Look: Another use for optogenetics and GPCR signaling.

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Which Fluorescent Protein Should I Use?

http://blog.addgene.org/which-fluorescent-protein-should-i-use

Technology Transfer / Licensing. Technology Transfer / Licensing. Which Fluorescent Protein Should I Use? On May 20, 2014 10:06:00 AM. Was contributed by Gal Haimovich of greenfluorescentblog. Be honest. Do you really know how fluorescent proteins glow? Fluorescence is the emission of light by a substance that has absorbed light. The emitted light is at a longer wavelength than the exciting wavelength. Thus, FPs are proteins with this unique capacity. The two most common uses are:. FPs are usually classi...

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SR Microscopy » 2014 Nobel Prize in Chemistry to be Awarded for Superresolution Microscopy

http://srmicroscopy.net/2014/10/08/2014-nobel-prize-in-chemistry-to-be-awarded-for-superresolution-microscopy

About Mad City Labs. What is SR Microscopy? 2014 Nobel Prize in Chemistry to be Awarded for Superresolution Microscopy. 2014 Nobel Prize in Chemistry to be Awarded for Superresolution Microscopy. October 8th, 2014. The Nobel Prize in Chemistry for 2014. Has been announced. It will be awarded to Eric Betzig, Stefan W. Hell, and William E. Moerner for the development of super-resolved fluorescence microscopy. Researchers in the large and growing community of scientists spearheading innovation in the field.

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Probing for Caveosomes | Rapha-z-lab-commons

https://raphazlabcommons.wordpress.com/2015/08/04/probing-for-caveosomes

August 4, 2015. August 13, 2015. As mentioned before, one of the things that has been bugging me about the SmartFlares is that they’re not where anyone expects them. EMD expect them in the cytosol (we don’t see them there), I expected them in endosomes and they weren’t really there either. So what could this mystery compartment be? There are not many papers dealing with the uptake of gold nanoparticle-conjugated nucleic acids. One from the Mirkin group. Supporting Information, Figure 5)…. Show cell-based...

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החיים המסתוריים של הספרים | הקוראת הראשונה

https://hakoret-harishona.com/2016/10/25/החיים-המסתוריים-של-הספרים

שלומית עוזיאל, עורכת תוכן ולשון. תכנים שלי במקומות אחרים. מה אני עושה: עריכת לשון ותוכן. האם זה חייב להיות כך לעזאזל? החיים המסתוריים של הספרים. אוקטובר 25, 2016. מאז ומעולם חשדו בני האדם שמילים כתובות אינן רק מילים כתובות: יש בהן כוח, ואולי אפילו חיים. ג'ון מילטון כתב על כך ב אריאופגיטיקה. שהרי ספרים אינם חפצים מתים לגמרי, ויש בהם מכוח החיים … אצורות בהם, כמו בקנקן חתום, העוצמה והתמצית הטהורות ביותר של האינטלקט החי אשר הוליד אותם. גזית פיזיקאי, המתבונן בספר זה בתחום הביולוגיה מציע שורת תכונות המהותיות, ...

srmicroscopy.net srmicroscopy.net

SR Microscopy » super-resolution microscopy

http://srmicroscopy.net/category/super-resolution-microscopy

About Mad City Labs. What is SR Microscopy? Archive for the ‘super-resolution microscopy’ Category. 2014 Nobel Prize in Chemistry to be Awarded for Superresolution Microscopy. October 8th, 2014. The Nobel Prize in Chemistry for 2014. Has been announced. It will be awarded to Eric Betzig, Stefan W. Hell, and William E. Moerner for the development of super-resolved fluorescence microscopy. From The Royal Swedish Academy of Sciences, “How the Optical Microscope Became a Nanoscope”. Disease. Eric Betzig ...

srmicroscopy.net srmicroscopy.net

SR Microscopy » New Nano-Cyte® Video

http://srmicroscopy.net/2014/03/07/new-nano-cyte-video-on-youtube

About Mad City Labs. What is SR Microscopy? March 7th, 2014. 3D Image Stabilization System. Video highlights some of the particle tracking and rendering capabilities of the standalone Nano-Cyte. Program, shown toward the end of the video. We are constantly expanding the capabilities and features of Nano-Cyte. Today to find out what Nano-Cyte. Can do for you. New molecular motion tracking mode for Nano-Cyte. Nano-Cyte Newsletter: Update on Nano-Cyte features. Valid XHTML 1.1.

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SR Microscopy » What is SR Microscopy?

http://srmicroscopy.net/what-is-super-resolution-microscopy

About Mad City Labs. What is SR Microscopy? What is SR Microscopy? October 5th, 2012. 2014 Nobel Prize in Chemistry to be Awarded for Superresolution Microscopy. New molecular motion tracking mode for Nano-Cyte. Nano-Cyte Newsletter: Update on Nano-Cyte features. The Nano-Cyte Concept: Image Centric Stabilization. Valid XHTML 1.1.

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SR Microscopy » instrumentation

http://srmicroscopy.net/category/instrumentation

About Mad City Labs. What is SR Microscopy? Archive for the ‘instrumentation’ Category. New molecular motion tracking mode for Nano-Cyte. August 6th, 2014. Single Molecule Imaging system now features a “tracking” mode capable of surveying a wide sample area. This new feature builds on the existing capabilities of the Nano-Cyte. March 7th, 2014. 3D Image Stabilization System. Video highlights some of the particle tracking and rendering capabilities of the standalone Nano-Cyte. Can do for you. Ability to l...

srmicroscopy.net srmicroscopy.net

SR Microscopy » microscope drift

http://srmicroscopy.net/tag/microscope-drift

About Mad City Labs. What is SR Microscopy? Posts Tagged ‘microscope drift’. An Image-Centric View of Microscopy. November 16th, 2012. It’s clear that microscope drift is a huge problem for super-resolution (SR) microscopy (see. Why Microscope Drift is Disastrous for Super-Resolution Microscopy. For example, if the image detector moves, the image would undergo a corresponding positional displacement. What is the reference frame you use to judge drift? The answer is to displace the sample by whatever amou...

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greenfluorescentblog | A blog on fluorescent microscopy in biological research

A blog on fluorescent microscopy in biological research. A molecular ribosome counting mechanism – where’s the data? January 15, 2018. A recent paper in Nature. From the lab of Pavel Baranov and collaborators suggests a new type of mechanism of translation regulation. However, After discussing this paper in a journal club today – I’m not convinced about their model. Continue reading →. MS2 mRNA imaging in yeast – problem solved. November 13, 2017. Continue reading →. November 6, 2017. Paper). In this...

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