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Greg Dijkman | Lifescience Laboratory Instrument Consultant

Lifescience Laboratory Instrument Consultant. To generate good scientific data there are many sample. Preparation steps, then data collection, and then software analysis steps. In most cases, the. Way to perform an experiment requires instruments and reagents from multiple different sources. There is no company that can be good at providing everything. Use this site to help make informed decisions before capital equipment purchases. Nanostring Gene Expression Service Offered at McGill JGH.

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Greg Dijkman | Lifescience Laboratory Instrument Consultant | gregdijkman.com Reviews

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Lifescience Laboratory Instrument Consultant. To generate good scientific data there are many sample. Preparation steps, then data collection, and then software analysis steps. In most cases, the. Way to perform an experiment requires instruments and reagents from multiple different sources. There is no company that can be good at providing everything. Use this site to help make informed decisions before capital equipment purchases. Nanostring Gene Expression Service Offered at McGill JGH.

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Greg Dijkman | Lifescience Laboratory Instrument Consultant | gregdijkman.com Reviews

https://gregdijkman.com

Lifescience Laboratory Instrument Consultant. To generate good scientific data there are many sample. Preparation steps, then data collection, and then software analysis steps. In most cases, the. Way to perform an experiment requires instruments and reagents from multiple different sources. There is no company that can be good at providing everything. Use this site to help make informed decisions before capital equipment purchases. Nanostring Gene Expression Service Offered at McGill JGH.

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gregdijkman.com

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C1000 cycler programs | Greg Dijkman

http://www.gregdijkman.com/node/27

Lifescience Laboratory Instrument Consultant. Here are some programs that are becoming more and more popular. There are a lot of steps involved in Sequenom and Illumina protocols so I have posted the validated protocols here. Simply copy them onto a USB memory key and then you can copy it into the C1000. Here is the Shrimp Alkaline Phosphatase protocol (SAP) This step digests all the unincorporated dNTPs. All downloadable documents have been used with permission from the respective owners.

2

Difference between Digital PCR and Droplet Digital PCR (ddPCR) | Greg Dijkman

http://www.gregdijkman.com/difference-between-digital-pcr-and-droplet-digital-pcr-ddpcr

Lifescience Laboratory Instrument Consultant. Next Gen Library Prep. Difference between Digital PCR and Droplet Digital PCR (ddPCR). How can PCR be digital? That is a good question, actually digital PCR has aspects of both conventional PCR and realtime qPCR. Most importantly, the DNA sample is separated into compartments, then standard endpoint PCR is run on each compartment, and fluorescence is detected using taqman hydrolysis probes that we are familiar with from qPCR. Digital PCR (PCR numerique en fra...

3

How to get the best results for fluorescent western blots | Greg Dijkman

http://www.gregdijkman.com/node/26

Lifescience Laboratory Instrument Consultant. How to get the best results for fluorescent western blots. The article published in Pharma has an excellent overview of how to get the best results for fluorescent westwern blot applications. If you have an imager with multiple excitation sources like a Versadoc 4000 or Versadoc 5000 or Pharos FX, then performing fluorescent blots is easy. It is very important to use a low fluorescent background membrane.

4

Downloads | Greg Dijkman

http://www.gregdijkman.com/downloads

Lifescience Laboratory Instrument Consultant. Droplet digital PCR (ddPCR). CFX96 Realtime PCR Brochure. Bioplex 5pl Curve Fitting. Pulsed Field Gel Electrophoresis. Pharos FX Laser Scanner. Droplet Digital PCR (ddPCR) Technology. Digital PCR used for quantification of Next Gen Sequencing Library (BMC Genomics). Vandesompele (Multiple Reference Genes). Pfaffl Gene Expression Quantitation. Probe based High Resolution Melt. Case study of experiment that does NOT meet MIQE guidelines. And how to fix it).

5

Support Articles | Greg Dijkman

http://www.gregdijkman.com/support

Lifescience Laboratory Instrument Consultant. Next Gen Library Prep. On these pages you will find useful support articles and equipment reviews that answer many common questions. Guest articles are written by individuals with great expertise in a particular subject area. Select from the menus on the left. All downloadable documents have been used with permission from the respective owners.

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Greg Dijkman | Lifescience Laboratory Instrument Consultant

Lifescience Laboratory Instrument Consultant. To generate good scientific data there are many sample. Preparation steps, then data collection, and then software analysis steps. In most cases, the. Way to perform an experiment requires instruments and reagents from multiple different sources. There is no company that can be good at providing everything. Use this site to help make informed decisions before capital equipment purchases. Nanostring Gene Expression Service Offered at McGill JGH.

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