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Halle Diary

The blog is an attempt to express myself from Halle, the beautiful city in the north-eastern part of Germany. Monday, 4 April 2011. Subscribe to: Posts (Atom).

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Halle Diary | halle-diary.blogspot.com Reviews

https://halle-diary.blogspot.com

The blog is an attempt to express myself from Halle, the beautiful city in the north-eastern part of Germany. Monday, 4 April 2011. Subscribe to: Posts (Atom).

INTERNAL PAGES

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Halle Diary: April 2011

http://www.halle-diary.blogspot.com/2011_04_01_archive.html

The blog is an attempt to express myself from Halle, the beautiful city in the north-eastern part of Germany. Monday, 4 April 2011. Subscribe to: Posts (Atom).

2

Halle Diary

http://www.halle-diary.blogspot.com/2011/04/under-construction.html

The blog is an attempt to express myself from Halle, the beautiful city in the north-eastern part of Germany. Monday, 4 April 2011. Subscribe to: Post Comments (Atom).

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labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: November 2008

http://labofphysiology.blogspot.com/2008_11_01_archive.html

The science and more. Tuesday, November 4, 2008. Gene silencing and siRNA. Once a protocol is optimized for a particular cell type, reproducible siRNA screening experiments can easily be done". Low transfection efficiency and low cell viability are the most frequent causes of unsuccessful gene silencing experiments. Through careful optimization- e.g. choosing the right transfection agent and transfection method- high levels of transfection efficiency can be achieved in many cell types.

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: DNA transfection using Lipofectamine

http://labofphysiology.blogspot.com/2008/10/dna-transfection-using-lipofectamine.html

The science and more. Monday, October 27, 2008. DNA transfection using Lipofectamine. Efficient DNA transfection to the cells. Here is the latest protocol for transfection of colon cancer cells:. Transfections of HCT116 using Lipofectamine 2000 and it worked perfectly in our experimental conditions. Let me outline my brief protocol (24-well plate design);. Day 1 (Seeding):-cell seeding at a density of 100,000 cells/well (cell should be 70-80% confluent on the day of transfection). I) Wash once with HBSSi...

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: October 2008

http://labofphysiology.blogspot.com/2008_10_01_archive.html

The science and more. Friday, October 31, 2008. Oral administration to rats. Are you guys doing some experiments really aware with the facts that what and why are you doing so? Most of us say/answer- YES, but actually NOT. Among many options, I prefer to visit BioForum. The page crowded with talents and more talents. Anyway, I was curious to know about Oral administration and some basic fact. Here is one good answer by SamOH. Monday, October 27, 2008. DNA transfection using Lipofectamine. Tube 1- 25 ul O...

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: SDS-PAGE; a nice animation by GeneEd Inc

http://labofphysiology.blogspot.com/2008/10/sds-page-nice-animation-by-geneed-inc.html

The science and more. Monday, October 27, 2008. SDS-PAGE; a nice animation by GeneEd Inc. Animation showing the process of SDS-PAGE for the separation of protein. October 31, 2008 at 12:34 AM. Got basic concept, its nice! December 21, 2010 at 3:12 AM. Subscribe to: Post Comments (Atom). Oral administration to rats. DNA transfection using Lipofectamine. SDS-PAGE; a nice animation by GeneEd Inc. Administration by Oral Gavage. RNAi discovered; a great tale and great animation.

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: August 2009

http://labofphysiology.blogspot.com/2009_08_01_archive.html

The science and more. Friday, August 21, 2009. Lentivirus transduction for stable knockdown of STAT-3. Should be treated as Risk Group Level 2 organisms for laboratory handling. All handling, storage and disposal of biohazard waste must be in accordance with University rules and regulations. 1) 48-well Tissue Culture Microplate. 3) Hexadimethrine bromide (Sigma). 4) Target Cell Line (MCF-7 and MCF-7/Adr breast cancer cells). 8) 50ml Falcon Tubes. 9) 15ml Falcon Tubes. 10) 15ml RNase Free Tubes. 2) Prepar...

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: Oral administration to rats

http://labofphysiology.blogspot.com/2008/10/oral-administration-to-rats.html

The science and more. Friday, October 31, 2008. Oral administration to rats. Are you guys doing some experiments really aware with the facts that what and why are you doing so? Most of us say/answer- YES, but actually NOT. Among many options, I prefer to visit BioForum. The page crowded with talents and more talents. Anyway, I was curious to know about Oral administration and some basic fact. Here is one good answer by SamOH. Subscribe to: Post Comments (Atom). Oral administration to rats.

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: MS and PhD studentships in Korea (updated for Fall 2011)

http://labofphysiology.blogspot.com/2009/09/phd-scholarships-in-korea.html

The science and more. Friday, October 7, 2011. MS and PhD studentships in Korea (updated for Fall 2011). Excellent opportunies for the Young Graduates. Write to Prof. JJ Shim. THE INSTITUTE OF CLEAN TECHNOLOGY. 214-1 Dae-dong, Gyeongsan, Gyeongbuk 712-749, KOREA. Tel : (53)810-2587 Fax : (53)810-4631 E-mail : jjshim@yu.ac.kr. Area of study: Polymers, Organic Chemistry, Inorganic Chemistry, Nano Technology, Clean Technology, Catalysis, Process Control, etc. Yeungnam University Chunma Art Center. MS and Ph...

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: RT-PCR protocols

http://labofphysiology.blogspot.com/2008/10/rt-pcr-protocols.html

The science and more. Monday, October 27, 2008. Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) and Other PCR Procedures. Department of Veterinary Science and Molecular Toxicology Program. To follow the Penn State Uni. Subscribe to: Post Comments (Atom). Oral administration to rats. DNA transfection using Lipofectamine. SDS-PAGE; a nice animation by GeneEd Inc. Administration by Oral Gavage. RNAi discovered; a great tale and great animation. Scratch wound Vs Transwell migration.

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: Lentivirus transduction for stable knockdown of STAT-3

http://labofphysiology.blogspot.com/2009/08/biohazard-should-be-treated-as-risk.html

The science and more. Friday, August 21, 2009. Lentivirus transduction for stable knockdown of STAT-3. Should be treated as Risk Group Level 2 organisms for laboratory handling. All handling, storage and disposal of biohazard waste must be in accordance with University rules and regulations. 1) 48-well Tissue Culture Microplate. 3) Hexadimethrine bromide (Sigma). 4) Target Cell Line (MCF-7 and MCF-7/Adr breast cancer cells). 8) 50ml Falcon Tubes. 9) 15ml Falcon Tubes. 10) 15ml RNase Free Tubes. 2) Prepar...

labofphysiology.blogspot.com labofphysiology.blogspot.com

Biology and Research: shRNA lentiviral particles

http://labofphysiology.blogspot.com/2009/08/shrna-lentiviral-particles.html

The science and more. Thursday, August 20, 2009. Lentiviral particles are widely available for targeted gene knockdown. Recently, I have set up gene knockdown experiment using Mission lentiviral particles from Sigma. I will share this information and basic protocol in next blog. Subscribe to: Post Comments (Atom). Lentivirus transduction for stable knockdown of ST. TE buffer (Tris-EDTA buffer) Recipe. Awesome Inc. template. Template images by molotovcoketail.

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Halle Diary

The blog is an attempt to express myself from Halle, the beautiful city in the north-eastern part of Germany. Monday, 4 April 2011. Subscribe to: Posts (Atom).

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