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The farrago | A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z]+)A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z]+)
http://haroldpimentel.wordpress.com/
A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z]+)
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The farrago | A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z]+) | haroldpimentel.wordpress.com Reviews
https://haroldpimentel.wordpress.com
A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z]+)
In RNA-Seq, 2 != 2: Between-sample normalization | The farrago
https://haroldpimentel.wordpress.com/2014/12/08/in-rna-seq-2-2-between-sample-normalization
A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z] ). December 8, 2014. In RNA-Seq, 2! In this post I will discuss between-sample normalization (BSN), which is often called library size normalization (LSN), though, I don’t particularly like that term. Here, I will refer to it as BSN. The post is guided towards scientists that don’t come from a mathematics background and people who are new to RNA-Seq. Not so random aside:. After many chats/rants with Nick Bray. Finding a “control se...
haroldpimentel | The farrago
https://haroldpimentel.wordpress.com/author/haroldpimentel
A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z] ). I'm a PhD candidate at UC Berkeley in the CS department working on statistics and interested in biology. The current state of R support in Atom. November 28, 2016. Bluetooth keyboard switch with Arduino. September 8, 2016. The power of randomness: Pham vs Gap Part II. January 22, 2015. Optimal “k” when there’s no cluster? Pham vs Gap Part I. December 29, 2014. In RNA-Seq, 2! December 8, 2014. A review of RNA-Seq expression units.
The power of randomness: Pham vs Gap Part II | The farrago
https://haroldpimentel.wordpress.com/2015/01/22/the-power-of-randomness-pham-vs-gap-part-ii
A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z] ). January 22, 2015. The power of randomness: Pham vs Gap Part II. This is part two of two looking at the performance of methods for determining the number of clusters in. Means clustering. For part one, see Optimal k when there’s no cluster? Pham vs Gap Part I. All of the examples, as well as plots can be found at this Github repo. In the previous post. We looked at how the gap statistic and Pham. Method, also by Tibshirani. Randomly pa...
What the FPKM? A review of RNA-Seq expression units | The farrago
https://haroldpimentel.wordpress.com/2014/05/08/what-the-fpkm-a-review-rna-seq-expression-units
A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z] ). May 8, 2014. A review of RNA-Seq expression units. This post covers the units used in RNA-Seq that are, unfortunately, often misused and misunderstood. I’ll try to clear up a bit of the confusion here. Throughout this post “read” refers to both single-end or paired-end reads. The concept of counting is the same with either type of read, as each read represents a fragment that was sequenced. Finally, I use the random variable. These nu...
Optimal “k” when there’s no cluster? Pham vs Gap Part I | The farrago
https://haroldpimentel.wordpress.com/2014/12/29/optimal-k-when-theres-no-cluster-gap-vs-pham-part-i
A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z] ). December 29, 2014. Optimal “k” when there’s no cluster? Pham vs Gap Part I. Clustering is a fairly common and useful algorithm. One of the difficulties in using. Means is choosing the number of clusters,. I use the gap statistic pretty regularly for choosing. In my analyses as I’ve found it to perform reasonably well in practice. Recently, I’ve been thinking a bit about clustering on Spark. Is the number of data points (sample size).
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RPKM, FPKM and TPM, clearly explained | Statquest
https://statquest.org/2015/07/09/rpkm-fpkm-and-tpm-clearly-explained
An epic journey through computational methods for molecular geneticists. RPKM, FPKM and TPM, clearly explained. RPKM, FPKM and TPM, clearly explained. July 9, 2015. July 22, 2015. Warning: This StatQuest is specifically for people who do high-throughput RNA sequencing (RNA-seq). If that’s what you’re interested in, quest on! These three metrics attempt to normalize for sequencing depth and gene length. Here’s how you do it for RPKM:. Count up all the RPK values in a sample and divide this number by 1,000...
“Alignment free” transcriptome quantification | Simon's Blog
https://sjcockell.me/2015/05/18/alignment-free-transcriptome-quantification
A bioinformatician's (infrequent) musings. 8220;Alignment free” transcriptome quantification. Last week a pre-print was published describing Kallisto. 8211; a quantification method for RNA-Seq reads described (by the authors) as ‘near optimal’. Here is Lior Pachter’s associated blog post. Kallisto follows a current trend for ‘alignment free’ quantification methods for RNA-Seq analysis, with recent methods including Sailfish. And it’s successor, Salmon. In Kallisto parlance,. Building a test set. Transcri...
BamHash and some insights from information theory | Bits of Bioinformatics
https://pmelsted.wordpress.com/2015/03/03/bamhash-and-some-insights-from-information-theory
Skip to primary content. BamHash and some insights from information theory. March 3, 2015. We have a new preprint. Out for a tool called BamHash. I’ll get to what it does and how it works in a bit, but first the why. This tool was built because we needed it at deCODE. Magic we get a shiny BAM file. To illustrate the question consider the following conversation. Is it safe to delete that FASTQ file? I just explained why it was safe why are you asking again? Is there something wrong with my pipeline? Can y...
March | 2015 | Bits of Bioinformatics
https://pmelsted.wordpress.com/2015/03
Skip to primary content. Skip to secondary content. Monthly Archives: March 2015. BamHash and some insights from information theory. March 3, 2015. We have a new preprint. Out for a tool called BamHash. I’ll get to what it does and how it works in a bit, but first the why. This tool was built because we needed it at deCODE. Magic we get a shiny BAM file. To illustrate the question consider the following conversation. Is it safe to delete that FASTQ file? Is there something wrong with my pipeline? No, you...
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The farrago | A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z]+)
A place for my thoughts on comp bio, statistics, cycling(, [a-zA-Z] ). November 28, 2016. The current state of R support in Atom. This is a guide to the current state of R support in Atom. I’m not writing this as an expert on Atom, merely as a user. This guide is geared towards Mac as I do not have experience on either Linux or Windows. All of these packages can be installed with the Atom package manager either via commandline (. Or in the GUI (. I am the maintainer of. The syntax highlighting for. Parse...
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