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Jake Heare Research Central

Jake Heare Research Central. Saturday, August 15, 2015. 8 15 2015 PGRP CTM2 reps qPCR. Today I ran replicates of all populations and all treatments to generate data about expression values. Here I ran PGRP. To save on cDNA I stopped running full replicates of the Control and Temperature samples with the mech stress samples. I run partials made up of 8 samples for Control and 8 of Heat. Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S). Repeat Elongation 39 times.

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Jake Heare Research Central. Saturday, August 15, 2015. 8 15 2015 PGRP CTM2 reps qPCR. Today I ran replicates of all populations and all treatments to generate data about expression values. Here I ran PGRP. To save on cDNA I stopped running full replicates of the Control and Temperature samples with the mech stress samples. I run partials made up of 8 samples for Control and 8 of Heat. Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S). Repeat Elongation 39 times.

<META> KEYWORDS

1 primers

2 pgrp s fwd

3 gagacttcacctcgcaccaa

4 olurida

5 pgrp s rev

6 aactggtttgcccgacatca

7 reagent table

8 volume

9 reactions x80

10 ssofast evagreen mm

CONTENT

Page content here

KEYWORDS ON PAGE

primers,pgrp s fwd,gagacttcacctcgcaccaa,olurida,pgrp s rev,aactggtttgcccgacatca,reagent table,volume,reactions x80,ssofast evagreen mm,fwd primer,rev primer,vortexed,centrifuged briefly,ran program below,program,step,temperature,time,initiation,elongation

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Jake Heare Research Central | heareresearch.blogspot.com Reviews

https://heareresearch.blogspot.com

Jake Heare Research Central. Saturday, August 15, 2015. 8 15 2015 PGRP CTM2 reps qPCR. Today I ran replicates of all populations and all treatments to generate data about expression values. Here I ran PGRP. To save on cDNA I stopped running full replicates of the Control and Temperature samples with the mech stress samples. I run partials made up of 8 samples for Control and 8 of Heat. Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S). Repeat Elongation 39 times.

INTERNAL PAGES

heareresearch.blogspot.com

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Jake Heare Research Central: 8 14 2015 GRB2 CTM reps qPCR

http://www.heareresearch.blogspot.com/2015/08/8-14-2015-grb2-ctm-reps-qpcr.html

Jake Heare Research Central. Friday, August 14, 2015. 8 14 2015 GRB2 CTM reps qPCR. Today I ran replicates of all populations and all treatments to generate data about expression values. Here I ran GRB2. Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2). Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2). Added reagents from greatest to least volume. Centrifuged plate at 2000 rpm for 1 minute. 55 - 95 C.

2

Jake Heare Research Central: 8 13 2015 PGEEP4 CTM reps qPCR

http://www.heareresearch.blogspot.com/2015/08/8-13-2015-pgeep4-ctm-reps-qpcr.html

Jake Heare Research Central. Friday, August 14, 2015. 8 13 2015 PGEEP4 CTM reps qPCR. Today I ran replicates of all populations and all treatments to generate data about expression values. Here I ran PGE/EP4. Prostaglandin E2 receptor EP4 subtype (PGE receptor EP4 subtype) (PGE2 receptor EP4 subtype) (Prostanoid EP4 receptor). Prostaglandin E2 receptor EP4 subtype (PGE receptor EP4 subtype) (PGE2 receptor EP4 subtype) (Prostanoid EP4 receptor). Added reagents from greatest to least volume. 55 - 95 C.

3

Jake Heare Research Central: 8 10 2015 EF1d Mech Stress 2 rep qPCR

http://www.heareresearch.blogspot.com/2015/08/8-10-2015-ef1d-mech-stress-2-rep-qpcr.html

Jake Heare Research Central. Tuesday, August 11, 2015. 8 10 2015 EF1d Mech Stress 2 rep qPCR. Today I ran mechanical stress samples in replicates of two to generate data about expression values. Here I ran EF1d . I ran NTCs on a separate plate using the same reaction volume and 9 ul of PCR water. Elongation factor 1-delta (EF-1-delta). Elongation factor 1-delta (EF-1-delta). Added reagents from greatest to least volume. Pipetted 11 ul Master Mix to each tube using a multichannel pipetter. 55 - 95 C.

4

Jake Heare Research Central: 8 14 2015 BMP2 CTM reps qPCR

http://www.heareresearch.blogspot.com/2015/08/8-14-2015-bmp2-ctm-reps-qpcr.html

Jake Heare Research Central. Friday, August 14, 2015. 8 14 2015 BMP2 CTM reps qPCR. Today I ran replicates of all populations and all treatments to generate data about expression values. Here I ran BMP2. Bone morphogenetic protein 2 (BMP-2) (Bone morphogenetic protein 2A) (BMP-2A). Bone morphogenetic protein 2 (BMP-2) (Bone morphogenetic protein 2A) (BMP-2A). Added reagents from greatest to least volume. Pipetted 11 ul Master Mix to each tube using a multichannel pipetter. Repeat Elongation 39 times.

5

Jake Heare Research Central: 8 14 2015 GRB2 CTM reps qPCR 2

http://www.heareresearch.blogspot.com/2015/08/8-14-2015-grb2-ctm-reps-qpcr-2.html

Jake Heare Research Central. Friday, August 14, 2015. 8 14 2015 GRB2 CTM reps qPCR 2. Today I ran replicates of all populations and all treatments to generate data about expression values. Here I ran GRB2. Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2). Growth factor receptor-bound protein 2 (Adapter protein GRB2) (Protein Ash) (SH2/SH3 adapter GRB2). Added reagents from greatest to least volume. Centrifuged plate at 2000 rpm for 1 minute. 55 - 95 C.

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Jake Heare Research Central

Jake Heare Research Central. Saturday, August 15, 2015. 8 15 2015 PGRP CTM2 reps qPCR. Today I ran replicates of all populations and all treatments to generate data about expression values. Here I ran PGRP. To save on cDNA I stopped running full replicates of the Control and Temperature samples with the mech stress samples. I run partials made up of 8 samples for Control and 8 of Heat. Peptidoglycan recognition protein 1 (Peptidoglycan recognition protein short) (PGRP-S). Repeat Elongation 39 times.

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