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Ion channels assays patch clamp. Action potential intracellular microelectrode. PhysioStim was created in 2000. On a sun- kissed ground where grows the pink garlic. They were only two at that time, now we are more than 11. Everything began in 2000. With the idea to raise a challenge: become the CRO. Independent, specialized in the field of the cardiac electrophysiology. Fifteen years later, PhysioStim is a niche CRO. Recognized as such through its expertise, its motivation and the loyalty of its customers.

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Ion channels assays patch clamp. Action potential intracellular microelectrode. PhysioStim was created in 2000. On a sun- kissed ground where grows the pink garlic. They were only two at that time, now we are more than 11. Everything began in 2000. With the idea to raise a challenge: become the CRO. Independent, specialized in the field of the cardiac electrophysiology. Fifteen years later, PhysioStim is a niche CRO. Recognized as such through its expertise, its motivation and the loyalty of its customers.

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1 start here

2 about us

3 overview

4 management

5 objectives

6 quality assurance

7 expertise

8 preclinical services

9 herg assay

10 hnav 1

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start here,about us,overview,management,objectives,quality assurance,expertise,preclinical services,herg assay,hnav 1,hkvlqt 1,mink,hcav 1,herg trafficking,isolated cardiomyocytes,human cardiomyocytes,guinea pig cardiomyocytes,purkinje fibres,rabbit atria

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Ion channels assays patch clamp. Action potential intracellular microelectrode. PhysioStim was created in 2000. On a sun- kissed ground where grows the pink garlic. They were only two at that time, now we are more than 11. Everything began in 2000. With the idea to raise a challenge: become the CRO. Independent, specialized in the field of the cardiac electrophysiology. Fifteen years later, PhysioStim is a niche CRO. Recognized as such through its expertise, its motivation and the loyalty of its customers.

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herg-assays.com

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hERG Trafficking

http://www.herg-assays.com/index.php/en/service-2/patch-clamp-2/inhibition-trafficking

Ion channels assays patch clamp. Action potential intracellular microelectrode. HERG trafficking inhibition on Human Embryonic Kidney ( HEK. Long term exposure of drugs to ion channel can lead to disruption of the trafficking of the ion channel to the cell surface membrane. It has been clearly demonstrated with the hERG channel where many of the LQT 2. Amplitude of the tail current upon repolarization from 70. Amplitude of the base current at 80. Inhibition of hERG tail current amplitude (%).

2

hKv1.5

http://www.herg-assays.com/index.php/en/service-2/patch-clamp-2/hkv1-5-en

Ion channels assays patch clamp. Action potential intracellular microelectrode. Assays on Human Embryonic Kidney ( HEK. Potassium channel encodes for the ultrarapid component of the delayed rectifier potassium channel IKur. In the cardiac tissue, K. Potassium channels are only expressed in atria in which it represents a major component of the cardiac repolarisation of atrial action potential. Thus K. Represents a major target for the treatment of atrial fibrillation, the most common sustained arrhythmia.

3

Compound Accumulation

http://www.herg-assays.com/index.php/en/service-2/langendorff/compound-accumulation

Ion channels assays patch clamp. Action potential intracellular microelectrode. Long perfusion of the compound in isolated Langendorff perfused hearts ( 2. Time dependent variation of left ventricular pressure and ECG. Calculation of pharmacokinetic parameters of accumulation using time- dependent formulation of the compound at the heart outflow. Amitriptyline: myocardial accumulation observed. Progressive increase in QTc interval during the 120. Fast increase in QTc interval, stable after 20.

4

Ion channels assays patch clamp

http://www.herg-assays.com/index.php/en/service-2/patch-clamp-2

Ion channels assays patch clamp. Action potential intracellular microelectrode. Conventional manual Patch Clamp in HEK- 293. The patch clamp technique. Is the gold standard method for assessing ion channel functionality and provides the most versatile and accurate assessment of drug interactions with ion channels. Two main configurations of the patch clamp technique are used at PhysioStim to record ion channel activity: the whole cell configuration and the perforated patch configuration.

5

Isolated Cardiomyocytes

http://www.herg-assays.com/index.php/en/service-2/isolated-cardiomyocytes

Ion channels assays patch clamp. Action potential intracellular microelectrode. Guinea- pig or human cardiomyocytes. Ventricular cardiomyocytes provide you a good solution to study drug effects on action potential i.e. on ion channels. This. Model can be useful for the evaluation of the cardiac safety of newly developed drugs according to the recommendations of the European Agency for the Evaluation of Medicinal Products. Human cardiomyocytes: isolated from IPS. Developped by Pcie .fr.

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Ion channels assays patch clamp. Action potential intracellular microelectrode. PhysioStim was created in 2000. On a sun- kissed ground where grows the pink garlic. They were only two at that time, now we are more than 11. Everything began in 2000. With the idea to raise a challenge: become the CRO. Independent, specialized in the field of the cardiac electrophysiology. Fifteen years later, PhysioStim is a niche CRO. Recognized as such through its expertise, its motivation and the loyalty of its customers.

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