hmec.lbl.gov

HMEC Extended Life Culture

Human Mammary Epithelial Cell (HMEC) Bank Website. As part of my ongoing research program since 1976, my laboratory has developed and stored frozen a variety of normal, benign, tumor-derived, and in vitro transformed cultures of HMEC. To facilitate scientific investigations using HMEC, our laboratory makes these cells available to other researchers. Descriptions of the cell types available can be found in Human Mammary Cell Types Generated. Section contains detailed protocols for how to grow these cells.

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HMEC Extended Life Culture | hmec.lbl.gov Reviews

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Human Mammary Epithelial Cell (HMEC) Bank Website. As part of my ongoing research program since 1976, my laboratory has developed and stored frozen a variety of normal, benign, tumor-derived, and in vitro transformed cultures of HMEC. To facilitate scientific investigations using HMEC, our laboratory makes these cells available to other researchers. Descriptions of the cell types available can be found in Human Mammary Cell Types Generated. Section contains detailed protocols for how to grow these cells.

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9 coupons

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HMEC Extended Life Culture | hmec.lbl.gov Reviews

https://hmec.lbl.gov

Human Mammary Epithelial Cell (HMEC) Bank Website. As part of my ongoing research program since 1976, my laboratory has developed and stored frozen a variety of normal, benign, tumor-derived, and in vitro transformed cultures of HMEC. To facilitate scientific investigations using HMEC, our laboratory makes these cells available to other researchers. Descriptions of the cell types available can be found in Human Mammary Cell Types Generated. Section contains detailed protocols for how to grow these cells.

INTERNAL PAGES

hmec.lbl.gov

1

A Brief History of HMEC Life, Aging, Death, and Immortality in ourCulture Systems

http://hmec.lbl.gov/mock/history.html

Human Mammary Cell Types Generated. An Overview of Growth, Aging, Senescence, and Immortality. An Overview of Growth, Aging, Senescence, and Immortality in our HMEC Culture System. Examination of these cultures has elucidated the many significant differences between normal and abnormal HMEC, and produced a new, molecularly defined model of the senescence barriers encountered by cultured HMEC ( Figure 1. We postulate that cultured finite lifespan HMEC encounter two mechanistically distinct barriers to ind...

2

REVIEW OF HMEC CULTURE SYSTEM

http://hmec.lbl.gov/mreview.htm

REVIEW OF HMEC CULTURE SYSTEM. I Tissue Derivation of HMEC Cultures. I A Tissue Procurement. I B Tissue Processing. II Growth of Finite Lifespan HMEC in vitro. IIA Media and Growth Capacity of Cultured HMEC. IIB Growth and Senescence of Cultured HMEC. Growth and Senescence of Pre-stasis HMEC. Growth and Senescence of Post-selection HMEC. IIC Growth after Inhibition of p53 Function. IID Characterization of Cultured HMEC Compared to Tissue of Origin. IIIA Derivation of Extended Life Cultures. IVA7 Telomera...

3

Human Mammary Cell Types Generated

http://hmec.lbl.gov/mock/descript.html

Human Mammary Cell Types Generated. An Overview of Growth, Aging, Senescence, and Immortality. HUMAN MAMMARY CELL TYPES GENERATED (see Chart 1. For more information on the derivation of these cell types. Live cells for distribution are sent frozen (in dry ice) in ampoules containing 5 x 10e5 or 1 x 10e6 cells. Under special circumstances, we can arrange to send growing cells in flasks. For some cell types, RNA is also available upon specific request. See REVIEW section VIII. A FINITE LIFESPAN HMEC. I mos...

4

Procedures

http://hmec.lbl.gov/other/procs.html

Human Mammary Cell Types Generated. An Overview of Growth, Aging, Senescence, and Immortality. Procedures for Culture of Human Mammary Epithelial Cells. I Cells Grown In a Serum Free Medium. A Making Media and Stocks. Serum-Free Supplement Stocks for MCDB 170. BPE (Bovine Pituitary Extract). EGF (Epidermal Growth Factor). B Growing and Subculturing Cells in Serum-Free Medium. Feeding and Plating Volumes and Densities. Seeding Cells From a Frozen Ampule. Freezing Cells for Liquid Nitrogen Storage. Also, t...

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hmec.lbl.gov

HMEC Extended Life Culture

Human Mammary Epithelial Cell (HMEC) Bank Website. As part of my ongoing research program since 1976, my laboratory has developed and stored frozen a variety of normal, benign, tumor-derived, and in vitro transformed cultures of HMEC. To facilitate scientific investigations using HMEC, our laboratory makes these cells available to other researchers. Descriptions of the cell types available can be found in Human Mammary Cell Types Generated. Section contains detailed protocols for how to grow these cells.

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