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Holly's Lab Book | My continuing adventures as a research scientist

My continuing adventures as a research scientist

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Holly's Lab Book | My continuing adventures as a research scientist | hollybik.wordpress.com Reviews
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My continuing adventures as a research scientist
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Holly's Lab Book | My continuing adventures as a research scientist | hollybik.wordpress.com Reviews

https://hollybik.wordpress.com

My continuing adventures as a research scientist

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Summarizing .biom table by sample name | Holly's Lab Book

https://hollybik.wordpress.com/2014/04/22/summarizing-biom-table-by-sample-name

Holly's Lab Book. My continuing adventures as a research scientist. Rerunning GOM Illumina on QIIME 1.8. Final Aquarium Project data now ready! Summarizing .biom table by sample name. April 22, 2014. This command collapsed replicates in the 18S chimera .biom table, pooling all reads from the same sample site together:. Note: I had to remove any samples that had zero representation in the .biom table (this included a couple of the GOM fungi replicates). Leave a Reply Cancel reply. Enter your comment here.

2

Rerunning GOM Illumina on QIIME 1.8 | Holly's Lab Book

https://hollybik.wordpress.com/2014/01/10/rerunning-gom-illumina-on-qiime-1-8

Holly's Lab Book. My continuing adventures as a research scientist. Filtering Fasta files in QIIME. Summarizing .biom table by sample name →. Rerunning GOM Illumina on QIIME 1.8. January 10, 2014. Pick open reference otus.py -i /home/ubuntu/data/GOM co ncat1.7 allR22combo 10Jan14.fna -o /home/ubuntu/data/uclust openref99 R22 10Jan -r /home/ubuntu/data/silva 111/99 Silva 111 rep set euk.fasta - parallel -O 8 -s 0.1 -p /home/ubuntu/data/qiime parameters 18Sopenref99 GOMamazon 16sept&#46...Got a weird error...

3

Filtering Fasta files in QIIME | Holly's Lab Book

https://hollybik.wordpress.com/2013/12/13/filtering-fasta-files-in-qiime

Holly's Lab Book. My continuing adventures as a research scientist. Aligning seqs on iMac. Rerunning GOM Illumina on QIIME 1.8 →. Filtering Fasta files in QIIME. December 13, 2013. Just discovered this script to filter my input fasta sequences in QIIME (e.g. post-demultiplexed samples with SampleId SeqID header format). Needed to do some filtering on the GOM Illumina data, as follows:. Filter fasta.py -f /Users/hollybik/Desktop/Data/Illumina GOM/demultiplexed qiime1.7/GOM concat1.7 fwd demult...Filter fa...

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QIIME | Holly's Lab Book

https://hollybik.wordpress.com/qiime

Holly's Lab Book. My continuing adventures as a research scientist. Quick Reference for running QIIME on Amazon AWS. QIIME 1.7.0 (release) AMI:. To mount an EBS volume:. Sudo mount /dev/xvdf /data. Sudo chown ubuntu /data. Sudo chgrp ubuntu /data. Quick Guide to Common Commands. Assigning Taxonomy to 18S sequences via RDP:. Parallel assign taxonomy rdp.py -i /Users/hol lybik/Downloads/Clustered OTUdata/QIIME/Uclust99 PrimerF04andNF1/OTUrepset uclu st99 F04NF1.fasta -o /Users/hollybik/Downloads/Cl...

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Final Aquarium Project data now ready! | Holly's Lab Book

https://hollybik.wordpress.com/2014/04/30/final-aquarium-project-data-now-ready

Holly's Lab Book. My continuing adventures as a research scientist. Summarizing .biom table by sample name. Final Aquarium Project data now ready! April 30, 2014. Got the demultiplexed data back from all 4 Aquarium project runs, and now we’re going forward with OTU picking. Starting off with closed ref, to see what we get:. Leave a Reply Cancel reply. Enter your comment here. Fill in your details below or click an icon to log in:. Address never made public). Notify me of new comments via email.

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Haute Science: Scientific Productivity Series: Evernote

http://scientificcouture.blogspot.com/2014/12/scientific-productivity-series-evernote.html

Tuesday, December 2, 2014. Scientific Productivity Series: Evernote. After seeing so many conversations on Twitter, I've decided to start a blog series about scientific productivity. This is something I think about ALL. THE. TIME. I'm constantly trying to make my work (and my life! And also this other one on Lifehacker. Yeah I'm a little addicted to Lifehacker). I've found Evernote to be invaluable for the following things:. Having a quick reference list for administrative details. I have notes from conf...

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Holly's Lab Book | My continuing adventures as a research scientist

Holly's Lab Book. My continuing adventures as a research scientist. Final Aquarium Project data now ready! April 30, 2014. Got the demultiplexed data back from all 4 Aquarium project runs, and now we’re going forward with OTU picking. Starting off with closed ref, to see what we get:. Summarizing .biom table by sample name. April 22, 2014. This command collapsed replicates in the 18S chimera .biom table, pooling all reads from the same sample site together:. Rerunning GOM Illumina on QIIME 1.8. I’v...

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