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积 会再来， 猴 住好礼. 请发送邮件至 oligo.china@genewiz.com.cn. 引物合成是一种化学合成，一般都采用β- 乙腈亚磷酰胺化学合成引物，合成时从3’ 5’方向进行，通常3’端的第一个碱基结合在CPG (Controlled Pore Glass，CPG)上。 我们的合成报告单给出了每OD引物稀释为100μmoL/L 即100pmol/ul 浓度的加水量，您可以根椐您的实验需要加入适量的无核酸酶的双蒸水 PH 6.0 或TE缓冲液 PH 7.5-8.0 ，开启瓶盖溶解之前最好在3000-4000转/分钟的转速下离心1分钟，防止开盖时引物散失。 没有溶解的引物非常稳定，可以在-20 下保存至少1年，溶解好的引物可以事先稀释为100μmoL/L的储存液，分装数管保存于-20 冰箱，可以保存至少半年以上 反复多次冻融会降低使用寿命。 如果需要计算合成DNA的精确浓度，请按以下公式进行计算 nmole数=(OD值x1000)/[ (15.3xA#) (7.4xC#) (11.8xG#) (9.3xT#) ]. 北京分公司 400-8100-669 天津分公司 022-59096300.
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