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Chromatography CourseBasic theory for reversed phase chromatography.
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Basic theory for reversed phase chromatography.
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Chromatography Course | hplccourse.com Reviews
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Basic theory for reversed phase chromatography.
hplccourse.com
Animated simulation
http://www.hplccourse.com/animatedsimulation.php
In chromatography different types of molecules are separated from each other as they migrates through a column. In the animation below red molecules are separated from blue in a simulation. Press the inject button to start an animated simulation of the chromatographic process.
Surface properties
http://www.hplccourse.com/surfacproperties.php
Here you find an introduction to the properties of silica and reversed phase surfaces. Many different types of non-polar stationary phase are available today. Historically, microparticulate silica to which alkyl chains were chemically bonded were the first type. It is still the most common type of reversed material. It offers a broad range of operating conditions to separate mixtures of molecules of different polarity. In the chemistry section. In the physical properties.
Retention theory
http://www.hplccourse.com/Retentiontheory.php
A large number of theoretical studies concerning the retention mechanism of reversed phase HPLC have been performed during the last thirty years. Despite all the efforts that have been made to understand the basics of the retention, an unified view has not been achieved. The manufacturing process of the silica and the type of alkyl ligands in the stationary phase. The type and composition of the mobile phase. The properties of the solute molecule that is used in the investigation. In the partition mechan...
HPLC Links
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Tutorials of basic chromatographic concepts is found in the site studyhplc.com. The site is divided into various sections depending on the level of knowledge: HPLC theory. Are the tutorials for different levels of knowledge at this site. Are different animations presented at this site. Test HPLC method developer. Are the different knowledge tests at this site. Is a list of the nomenclature used in chromatography. Other educative links are:. James K. Hardy. Has an illustration of TLC.
Thermodynamics
http://www.hplccourse.com/thermodynamics.php
The distribution of the solute between the mobile and stationary phase is the origin of retention of a solute in chromatography, see e.g. the animation. Under normal chromatographic conditions this distribution is approximately equal to the equilibrium distribution. The equilibrium constant for the distribution is proportional to the retention factor of the solute, i.e.:. K = retention factor ( sometimes also called the capacity factor ). The column phase ratio. T = the temperature in the system. Mainly ...
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Chromatography Course
LIQUID CHROMATOGRAPHY; SCIENCE OR ART? When working with method development or trouble shooting of liquid chromatographic methods, one usually comes to a point where the method is not working as well as one would like it too. The action taken is often to try to solve a problem by testing more or less vague ideas. The reasoning, “Maybe the problem can be solved if I change this or that parameter? One may ask; “Is this the most rational approach to problem solving in liquid chromatography? In reversed phas...
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Please Do Not Change Signage on 5th floor. Please do not make alterations to the endcap signs on 5. Floor without contacting a CLST supervisor or 5. Floor senior CSC first? There have been some signs altered and scribbled over; while the intent is to be helpful, it's making it more difficult to shelve/search for items. Thanks for your teamwork! Skype Room 226 Not Sending Print Job to CEN Library Update: Now Fixed. For refresher, here is how to find out where the print release station is, see the link here.
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