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HPLC HINTS and TIPS FOR CHROMATOGRAPHERS

A hints and tips blog about HPLC UHPLC chromatography LC/MS LC-MS SFC and the separation sciences in general. Provided by William Letter.

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A hints and tips blog about HPLC UHPLC chromatography LC/MS LC-MS SFC and the separation sciences in general. Provided by William Letter.
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HPLC HINTS and TIPS FOR CHROMATOGRAPHERS | hplctips.blogspot.com Reviews

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A hints and tips blog about HPLC UHPLC chromatography LC/MS LC-MS SFC and the separation sciences in general. Provided by William Letter.

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HPLC HINTS and TIPS FOR CHROMATOGRAPHERS: May 2015

https://hplctips.blogspot.com/2015_05_01_archive.html

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS. Translator for HPLC HINTS and TIPS for Chromatographers. Saturday, May 30, 2015. HPLC Peak Tailing - Some of the Most Common Reasons For it. Three easy ways to minimize chromatography peak tailing:. 3) Always use a freshly washed and equilibrated column. Is the column fouled or the inlet frit dirty? In concentration) than your mobile phase to wash off any strongly retained material after each run. For example, if you normally end a method with an 80% concentr...

2

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS: April 2015

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HPLC HINTS and TIPS FOR CHROMATOGRAPHERS. Translator for HPLC HINTS and TIPS for Chromatographers. Saturday, April 4, 2015. Standard Addition HPLC Calibration Method (Spiking). Due to the change in sample concentration. The fortified standards created by spiking the original samples is useful when no version of the sample matrix is available without the analyte for use as a true blank. Common “Spiking” Standard Addition Procedure Example:. Links to this post. Subscribe to: Posts (Atom). An expert Chromat...

3

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS: INLINE HPLC DEGASSING MODULES

https://hplctips.blogspot.com/2011/02/inline-hplc-degassing-modules.html

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS. Translator for HPLC HINTS and TIPS for Chromatographers. Wednesday, February 16, 2011. INLINE HPLC DEGASSING MODULES. INLINE HPLC DEGASSER / DEGASSING MODULES:. The volume of liquid contained in the tubing and vacuum chamber of EACH. This process takes time and should be performed on every channel in your system, even if you are not going to use them all. How much volume you ask? November 15, 2015 at 6:05 PM. We were puzzled by why our gradients would never run r...

4

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS: Standard Addition HPLC Calibration Method (Spiking)

https://hplctips.blogspot.com/2015/04/standard-addition-hplc-calibration.html

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS. Translator for HPLC HINTS and TIPS for Chromatographers. Saturday, April 4, 2015. Standard Addition HPLC Calibration Method (Spiking). Due to the change in sample concentration. The fortified standards created by spiking the original samples is useful when no version of the sample matrix is available without the analyte for use as a true blank. Common “Spiking” Standard Addition Procedure Example:. October 21, 2015 at 7:14 PM. October 31, 2015 at 11:27 AM. Ala: I...

5

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS: HPLC Peak Tailing - Some of the Most Common Reasons For it

https://hplctips.blogspot.com/2015/05/hplc-peak-tailing-most-common-reasons.html

HPLC HINTS and TIPS FOR CHROMATOGRAPHERS. Translator for HPLC HINTS and TIPS for Chromatographers. Saturday, May 30, 2015. HPLC Peak Tailing - Some of the Most Common Reasons For it. Three easy ways to minimize chromatography peak tailing:. 3) Always use a freshly washed and equilibrated column. Is the column fouled or the inlet frit dirty? In concentration) than your mobile phase to wash off any strongly retained material after each run. For example, if you normally end a method with an 80% concentr...

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HPLC HINTS and TIPS FOR CHROMATOGRAPHERS. Translator for HPLC HINTS and TIPS for Chromatographers. Saturday, March 10, 2018. Vespel, Tefzel or PEEK Valve Rotor Seals? One of the most common HPLC preventative maintenance parts is the injector valve rotor seal. The choice of rotor seal material should be based on: (1) the types of chemicals it will come into contact with; (2) the working pH range; (3) the temperature range. Common HPLC Rotor Seal Material Types:. Chemical name, ethylene-tetrafluoroethylene...

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