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digital based alu-HIV PCR for quantification of integrated proviral HIV DNAMethod for digital PCR based data analysis of the repetitive Alu-HIV PCR using Poisson statistics
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Method for digital PCR based data analysis of the repetitive Alu-HIV PCR using Poisson statistics
http://integratedhivpcr.ugent.be/
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digital based alu-HIV PCR for quantification of integrated proviral HIV DNA | integratedhivpcr.ugent.be Reviews
https://integratedhivpcr.ugent.be
Method for digital PCR based data analysis of the repetitive Alu-HIV PCR using Poisson statistics
integratedhivpcr.ugent.be
digital based alu-HIV PCR for quantification of integrated proviral HIV DNA
http://www.integratedhivpcr.ugent.be/protocol.html
Protocol for Quantification of Integrated HIV DNA. Authors: Sofie Rutsaert, Eva Malatinkova, Pawel Bonczkowski, Maja Kiselinova, Ward De Spiegelaere. DNA extraction kit (Qiagen DNeasy Blood and Tissue kit, cat. 69504/69506). GoTaq G2 DNA Polymerase (Promega, cat. M7845). Nuclease-free water (Qiagen, cat. 129114). DNTP mix (Promega, cat. U1511). Primers (see Table 1, ordered at Integrated DNA Technologies). LightCycler 480 Probes Master, 2x concentrated (Roche Applied Science, cat. 04707494001). Repeat se...
references to work about quantification of integrated proviral HIV DNA
http://www.integratedhivpcr.ugent.be/links.html
Website of our research unit. HIV Cure Research Center. Donate to HIV research. Website of the department of Internal Medicine at Ghent University.
references to work about quantification of integrated proviral HIV DNA
http://www.integratedhivpcr.ugent.be/news.html
29 July 2015 -. We organize a symposium on HIV Reservoir Characterization and a workshop. On digital PCR at Ghent University between 19-22 September 2016.
references to work about quantification of integrated proviral HIV DNA
http://www.integratedhivpcr.ugent.be/references.html
Butler SL, Hansen MST, Bushman FD: A quantitative assay for HIV DNA integration in vivo. Nat Med 2001;7:631-4. url. Brady T, Kelly BJ, Male F, Roth S, Bailey A, Malani N, Gijsbers R, O'Doherty U, Bushman FD: Quantitation of HIV DNA integration: Effects of differential integration site distributions on Alu-PCR assays. J. Virol. Methods 2013;189:53-7. url. Brussel A, Delelis O, Sonigo P: Alu-LTR real-time nested PCR assay for quantifying integrated HIV-1 DNA. Methods Mol Biol 2005;304:139-54. url. O'Dohert...
digital based alu-HIV PCR for quantification of integrated proviral HIV DNA
http://www.integratedhivpcr.ugent.be/index.html
Poisson analysis of the Alu-HIV PCR. The Alu HIV PCR is the method of choice for quantification of integrated HIV DNA in cellular genomes see also (Liszewski et al., 2009). For an in depth protocol of the method. by performing a repetitive sampling strategy with more than 40 replicate PCR reactions, the data can be assessed in a similar manner as digital PCR data. To enable data analysis of this repetitive sampling Alu-HIV PCR method we provide an Excel template. Ward De Spiegelaere Ghent University.
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digital PCR and quantitative PCR summer course for HIV reservoir research at Ghent University
http://www.htru.ugent.be/pcrcourse
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digital based alu-HIV PCR for quantification of integrated proviral HIV DNA
Poisson analysis of the Alu-HIV PCR. The Alu HIV PCR is the method of choice for quantification of integrated HIV DNA in cellular genomes see also (Liszewski et al., 2009). For an in depth protocol of the method. by performing a repetitive sampling strategy with more than 40 replicate PCR reactions, the data can be assessed in a similar manner as digital PCR data. To enable data analysis of this repetitive sampling Alu-HIV PCR method we provide an Excel template. Ward De Spiegelaere Ghent University.
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