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Jonathan Allen Capstone Research

Jonathan Allen Capstone Research. Tuesday, December 1, 2015. Concluding remarks on my Capstone and a link to the final paper. Objective: A final update on the research. A link to the paper is below covering the behavioral study and Luciferase production. Https:/ docs.google.com/document/d/1cmJKgJZ55wJTxqHJyjghMW-6cflsoIo8eL9vNCIkAy8/edit? Wednesday, November 18, 2015. Take cDNA and perform a qPCR to quantify gene expression for 3 sets of samples amplified using 2 different primers. Pipettes (10 - 1000uL).

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Jonathan Allen Capstone Research | jda26.blogspot.com Reviews

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Jonathan Allen Capstone Research. Tuesday, December 1, 2015. Concluding remarks on my Capstone and a link to the final paper. Objective: A final update on the research. A link to the paper is below covering the behavioral study and Luciferase production. Https:/ docs.google.com/document/d/1cmJKgJZ55wJTxqHJyjghMW-6cflsoIo8eL9vNCIkAy8/edit? Wednesday, November 18, 2015. Take cDNA and perform a qPCR to quantify gene expression for 3 sets of samples amplified using 2 different primers. Pipettes (10 - 1000uL).

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Jonathan Allen Capstone Research | jda26.blogspot.com Reviews

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Jonathan Allen Capstone Research. Tuesday, December 1, 2015. Concluding remarks on my Capstone and a link to the final paper. Objective: A final update on the research. A link to the paper is below covering the behavioral study and Luciferase production. Https:/ docs.google.com/document/d/1cmJKgJZ55wJTxqHJyjghMW-6cflsoIo8eL9vNCIkAy8/edit? Wednesday, November 18, 2015. Take cDNA and perform a qPCR to quantify gene expression for 3 sets of samples amplified using 2 different primers. Pipettes (10 - 1000uL).

INTERNAL PAGES

jda26.blogspot.com

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Jonathan Allen Capstone Research: April 2015

http://jda26.blogspot.com/2015_04_01_archive.html

Jonathan Allen Capstone Research. Thursday, April 23, 2015. Objective: Run PCR product out on a gel to verify that the Luciferase cDNA was succesfully replicated. Results: Controls in lane 4 and 5 show contamination so will redo pcr with fresh ingredients (primers, h20, apex red etc). Ladder: o'gene ruler 100bp #sm1143 lot 00189314 0.1 ug/ul. 1 5 ul Ladder. 2 23ul cDNA 1. 3 23ul CDNA 2. 4 23ul H20 1 (control). 5 23ul H20 2 (control). Wednesday, April 22, 2015. Use to bring reaction volume up to 25uL.

2

Jonathan Allen Capstone Research: August 2014

http://jda26.blogspot.com/2014_08_01_archive.html

Jonathan Allen Capstone Research. Friday, August 8, 2014. The enclosure is complete setup and put together as well as the artemia enclosure. I placed 7 2 gallon buckets in the bottom with sifted find sand donated by the fisheries department. A timer with light has been setup but will need to be worked with to make sure it is on the correct light cycle. Subscribe to: Posts (Atom). View my complete profile. Watermark theme. Powered by Blogger.

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Jonathan Allen Capstone Research: Primers for luciferase gene expression experiment.

http://jda26.blogspot.com/2015/10/primers-for-luciferase-gene-expression.html

Jonathan Allen Capstone Research. Friday, October 2, 2015. Primers for luciferase gene expression experiment. Objective: To design primers from the consensus sequence derived from multiple sequences produced from P. gurneyi samples. Primer pgurneyi 2 Cover opposite ends of the. Primer pgurneyi 2 fwd. Primer pgurneyi 2 rev. Primer pgurneyi 1 fwd. Primer pgurneyi 1 rev. Sequences used for Consensus sequence. Sequences used for Consensus sequence. Primers options I used 1 and 8. View my complete profile.

4

Jonathan Allen Capstone Research

http://jda26.blogspot.com/2015/10/objective-take-tissue-samples-i.html

Jonathan Allen Capstone Research. Friday, October 30, 2015. Read the SOPs for each of the reagents used in this protocol. Read the manufacturer's RNAzol RT protocol for Total RNA Isolation. Verify sufficient quantities/availability of reagents/equipment. Wear clean gloves and change gloves frequently. Aliquot 500uL of RNAzol RT to pestle tubes and store on ice. Transfer tissue to pestle tubes containing RNAzol RT. Homogenize immediately with disposable pestle. Add 400uL of 0.1% DEPC-treated H2O. OR rando...

5

Jonathan Allen Capstone Research: May 2015

http://jda26.blogspot.com/2015_05_01_archive.html

Jonathan Allen Capstone Research. Wednesday, May 27, 2015. Objective: Start DNA extraction on sample "night 2" an additional tissue sample taken during the manipulation experiment. DNA extraction preformed with E.Z.N.A. mollusk dna kit. Methods: E.Z.N.A. Mollusc DNA Protocol. Materials and Equipment to be Supplied by User: •. Before Starting: • Prepare buffers according to the instructions on Page 4 • Set water baths to 60°C and 70°C • Heat Elution Buffer to 70°C 1. 1 Pulverize no more than 50 mg of tiss...

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Sea Pens: Light on the Seafloor

https://marinebiology.uw.edu/news-stories/2015/10/09/sea-pens-light-on-the-seafloor

Skip to main content. Skip to footer unit links. Aquatic and Fishery Sciences. Faculty & Instructors. Field Stations and Ships. Plan a Quarter at FHL. Advice for Future Marine Biologists. Opportunities for K-12 Students. News & Stories. College of the Environment. Aquatic and Fishery Sciences. Faculty & Instructors. Field Stations and Ships. Plan a Quarter at FHL. Advice for Future Marine Biologists. Opportunities for K-12 Students. News & Stories. Oct 9, 2015. Research and Faculty Spotlight. He is still...

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Jonathan Allen Capstone Research

Jonathan Allen Capstone Research. Tuesday, December 1, 2015. Concluding remarks on my Capstone and a link to the final paper. Objective: A final update on the research. A link to the paper is below covering the behavioral study and Luciferase production. Https:/ docs.google.com/document/d/1cmJKgJZ55wJTxqHJyjghMW-6cflsoIo8eL9vNCIkAy8/edit? Wednesday, November 18, 2015. Take cDNA and perform a qPCR to quantify gene expression for 3 sets of samples amplified using 2 different primers. Pipettes (10 - 1000uL).

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