knockout.cwru.edu
Case Transgenic And Targeting FacilityTransgenic Mice and Knockout Mice: Transgenic Facility; Transgenic Core
http://knockout.cwru.edu/
Transgenic Mice and Knockout Mice: Transgenic Facility; Transgenic Core
http://knockout.cwru.edu/
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Case Transgenic And Targeting Facility | knockout.cwru.edu Reviews
https://knockout.cwru.edu
Transgenic Mice and Knockout Mice: Transgenic Facility; Transgenic Core
knockout.cwru.edu
Embryo Cryopreservation
http://knockout.cwru.edu/services/cryoembryo.shtml
What We Will Provide Part I. Hormones for injection, and instructions for tissue collection. What You Will Provide. The mice to be cryopreserved. Hormone injection, mating and tissue collection coordinated with core. What We Will Provide Part II. Cryopreservation of 225 embryos or more ( 25 embryos per straw). Long term storage in liquid nitrogen included in cost at this time. Test thaws to birth ( FET. Are available for an extra charge. You will need the following information to order. We expect that ea...
Other Services
http://knockout.cwru.edu/services/other.html
We are happy to perform additional services to aid you in your experiments. Please contact us to discuss what we can do for you. Our goal is to assist you. CRISPR/Cas Design and Validation. Ovariectomy, Ovary Transplantation, Vasectomy And Other Surgery. There is a minimum charge of $600 per service. Contact us for details. Aggregation And Tetraploid Chimeras. 3,000 for three sessions. Contact us for details.
Rederivation
http://knockout.cwru.edu/services/rederivation.shtml
What You Will Provide. The mice to be rederived- a minimum of 3 (maximum of 10) fertile, carrier males and the purchase of females to superovulate. What We Will Provide. A detailed schedule proposal with specific instructions regarding the purchase of donor females and preparation of males. Hormones, hormone injection and mating of the mice to be rederived. Transfer of the embryos to recipients. Our goal is to provide you with sufficient rederived mice of the correct genotype to reestablish the strain.
Chimeras
http://knockout.cwru.edu/services/chimeras.shtml
What You Will Provide Part I. A mycoplasma-free ES cell line with 40 chromosomes. What We Will Do. We will inject approximately 50 blastocysts per ES cell line. This should result in 10 mice born. Three of the mice are expected to be strong male chimeras. We have a proven record of success. We will house the mice until weaning (three weeks after birth). What You Will Provide Part II. You will house the weaned mice. 3,000 per 129 cell line, $4,500 per cell line for other genetic backgrounds. Whether the g...
Wait Times And Timelines for Service
http://knockout.cwru.edu/info/waittimes.shtml
Wait Times and Timelines for Service. Wait Times and Timelines (8/28/16):. From the start of rederivation, it will be a minimum of 6 weeks before we will be able to deliver mice to you (almost 3 weeks for gestation, and at least 3 weeks of postnatal growth before weaning). The time to cryopreserve sufficient numbers of high quality embryos is dependent on the size of the colony and the fecundity of the strain.
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Case Transgenic And Targeting Facility
Fundamentals of Mouse Biology and Genetics. Practical Mouse Colony Management. Breeding Strategies (.pdf, Jax). BAC Transgenic, Transgenic or Targeted Mice? Ringwald/Eppig mouse database primer (.pdf). Breeding, Care And Maintenance Of Mice. Plasmid Transgene DNA Purification. Purification Of BAC DNA For BAC Transgenics. Detecting Transgenes by PCR and Southern Blot. Preparing Earpunch DNA For PCR Genotyping. Preparing Mouse DNA For Southern Blots. Performing Gavage on Mice. Making ES Cells Details.
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