lab-insilico.blogspot.com

DBT-Mbiologist

Tuesday, March 23, 2010. 8212; Knowledge of plant and animal organisms, their tissues, cells, functions, interdependencies, and interactions with each other and the environment. 8212; Knowledge of the chemical composition, structure, and properties of substances and of the chemical processes and transformations that they undergo. This includes uses of chemicals and their interactions, danger signs, production techniques, and disposal methods. 8212; Giving full attention to what other people are saying, t...

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DBT-Mbiologist | lab-insilico.blogspot.com Reviews

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Tuesday, March 23, 2010. 8212; Knowledge of plant and animal organisms, their tissues, cells, functions, interdependencies, and interactions with each other and the environment. 8212; Knowledge of the chemical composition, structure, and properties of substances and of the chemical processes and transformations that they undergo. This includes uses of chemicals and their interactions, danger signs, production techniques, and disposal methods. 8212; Giving full attention to what other people are saying, t...

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1 dbt mbiologist

2 vk singh profile

3 knowledge

4 biology

5 chemistry

6 mathematics

7 english language

8 education and training

9 administration and management

10 computers and electronics

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DBT-Mbiologist | lab-insilico.blogspot.com Reviews

https://lab-insilico.blogspot.com

Tuesday, March 23, 2010. 8212; Knowledge of plant and animal organisms, their tissues, cells, functions, interdependencies, and interactions with each other and the environment. 8212; Knowledge of the chemical composition, structure, and properties of substances and of the chemical processes and transformations that they undergo. This includes uses of chemicals and their interactions, danger signs, production techniques, and disposal methods. 8212; Giving full attention to what other people are saying, t...

INTERNAL PAGES

lab-insilico.blogspot.com

1

DBT-Mbiologist: Common Buffer

http://lab-insilico.blogspot.com/2010/03/common-buffer.html

Thursday, March 11, 2010. PHEM (500 mls) 2x. PH 70 w/ KOH. PBS (5x in 500 mls). 20mM Tris pH 8.0. 100 ml 1 M PIPES (100 mM PIPES pH= 6.9). 2 ml 1 M MgSO. 2 ml 0.5 M EGTA (1 mM EGTA,). Distilled water to 900 ml. Adjust pH to 6.9. Distilled water to 1 liter. Put 50 g Vaseline, 50 g lanolin, and 50 g paraffin (all available from Fisher) in a 1 L Pyrex beaker. Heat on "low" setting on a hotplate, stirring occasionally, until all components are melted and well mixed. Store at room temperature. 4 ml of 1 M MgSO.

2

DBT-Mbiologist: Protocol Online: Cached

http://lab-insilico.blogspot.com/2010/03/protocol-online-cached_11.html

Thursday, March 11, 2010. 3M NaOAc (5.2). NaOAc3H2O 40.81 g. Adjust pH to 5.2 with glacial HOAc, adjust volume to 100 ml with DD-H2O, and autoclave to sterilize. Bromophenol Blue .025 g. Xylene Cyanole .025 g. Ficoll 400 1.5 g. DD-H2O to 10 ml. May be stored at room temp or at 4 degrees. 1M Tris (per 500 ml). Tris Base 60.55 g. Dissolve and adjust to the desired pH by adding concentrated HCl. Adjust volume to 500 ml with DD-H2O. 05M EDTA (8.0) (per liter). EDTA2H2O(disodium salt) 186.1 g. Tris Base 242 g.

3

DBT-Mbiologist: March 2010

http://lab-insilico.blogspot.com/2010_03_01_archive.html

Tuesday, March 23, 2010. 8212; Knowledge of plant and animal organisms, their tissues, cells, functions, interdependencies, and interactions with each other and the environment. 8212; Knowledge of the chemical composition, structure, and properties of substances and of the chemical processes and transformations that they undergo. This includes uses of chemicals and their interactions, danger signs, production techniques, and disposal methods. 8212; Giving full attention to what other people are saying, t...

4

DBT-Mbiologist: Make their Own Polymerase

http://lab-insilico.blogspot.com/2010/03/make-their-own-polymerase.html

Tuesday, March 9, 2010. Make their Own Polymerase. Make your own Taq polymerase? Can fidelity be improved by altering the purification method of DNA polymerases? During this past month some netters were discussing the hypothetical isolation and purification of Taq polymerase for in-house use. Should you make your own polymerase? To do this would require a tremendous effort and many netters think that the cost of doing it might not be worth all the trouble, since high-quality enzyme is available from many...

5

DBT-Mbiologist: STOCK SOLUTIONS AND MEDIA

http://lab-insilico.blogspot.com/2010/03/stock-solutions-and-media.html

Thursday, March 11, 2010. STOCK SOLUTIONS AND MEDIA. STOCK SOLUTIONS AND MEDIA. STOCK SOLUTIONS AND MEDIA. 200 ml, 500 ml, or 950 ml water. Add 198 ml water. Add 188 ml water. Anhydrous) 2.4 g. Add 200 ml water. Glutamic acid (Na) 20.0 g. Add 188 ml water. Glucose(dextrose) 80.0 g. Add water to 200 ml. Casein Acid Hydrolysate 20.0 g. Add 186 ml water. Yeast extract 20.0 g. Add 150-160 ml water. Autoclave. Add 150 ul ß-mercaptoethanol. Tryptone 40.0 g. NaCl 20.0 g. Add 190 ml water. Autoclave. Add 196 ml H.

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DBT-Mbiologist

Tuesday, March 23, 2010. 8212; Knowledge of plant and animal organisms, their tissues, cells, functions, interdependencies, and interactions with each other and the environment. 8212; Knowledge of the chemical composition, structure, and properties of substances and of the chemical processes and transformations that they undergo. This includes uses of chemicals and their interactions, danger signs, production techniques, and disposal methods. 8212; Giving full attention to what other people are saying, t...

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