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Isolation of DNA from Environmental Surface Water Samples. Isolation of DNA from Environmental Surface Water Samples. 1 Before starting the experiment take out solution I from the freezer and prepare solution II and collect ice. 2 By shaking the water container take 1.5 ml (750 ml 750 ml) water into eppendorf tube. 3 Centrifuge at 14, 000 rpm for 5-6 minutes. 4 Decant supernatant (SNT) and dry on tissue paper. 5 Add 100 ml Solution I in each tube and mix by pipetting. 10 Collect SNT with cut tips. 21 Inc...

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Lab Recepies | labrecepies.blogspot.com Reviews

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Isolation of DNA from Environmental Surface Water Samples. Isolation of DNA from Environmental Surface Water Samples. 1 Before starting the experiment take out solution I from the freezer and prepare solution II and collect ice. 2 By shaking the water container take 1.5 ml (750 ml 750 ml) water into eppendorf tube. 3 Centrifuge at 14, 000 rpm for 5-6 minutes. 4 Decant supernatant (SNT) and dry on tissue paper. 5 Add 100 ml Solution I in each tube and mix by pipetting. 10 Collect SNT with cut tips. 21 Inc...

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Lab Recepies | labrecepies.blogspot.com Reviews

https://labrecepies.blogspot.com

Isolation of DNA from Environmental Surface Water Samples. Isolation of DNA from Environmental Surface Water Samples. 1 Before starting the experiment take out solution I from the freezer and prepare solution II and collect ice. 2 By shaking the water container take 1.5 ml (750 ml 750 ml) water into eppendorf tube. 3 Centrifuge at 14, 000 rpm for 5-6 minutes. 4 Decant supernatant (SNT) and dry on tissue paper. 5 Add 100 ml Solution I in each tube and mix by pipetting. 10 Collect SNT with cut tips. 21 Inc...

INTERNAL PAGES

labrecepies.blogspot.com

1

Lab Recepies: Stripping buffer for Western blot

http://labrecepies.blogspot.com/2008/06/stripping-buffer-for-western-blot.html

Stripping buffer for Western blot. Stripping buffer (for 500 ml):. Tris- - - - - - - - - - - - - - -4.925 g. SDS- - - - - - - - - - - - - - -10 g. 2-Mercaptoethanol (ME)- - - - 3.52 ml. Distilled Water (D/W)- - - - - 500 ml. 1 Take 4.925 g Tris in a 500 ml cylinder. 2 Add 300 ml distilled water and dissolve using a magnetic stirrer. 3 Adjust pH 6.7 6.8 with concentrated HCl (handle cautiously). 5 Take out the magnet from the cylinder and volume up to 500 ml with DW. January 15, 2014 at 12:06 AM.

2

Lab Recepies: Western Blot

http://labrecepies.blogspot.com/2008/06/western-blot.html

Cut PVDF membrane: 8.5 cm x 9.5 cm size. Cut blotting paper: 10 cm x 10 cm size. 1 First sock the PVDF membrane in 100% methanol (MeOH). 2 Immediately transfer in a new plastic box and socked by dipping in solution B (for 1 gel use 1 membrane). 3 Then sock the membrane maximum 1 hr gently @ RT in shaker. 4 After gel run (when the tracking dye comes out stop the run) [wear gloves or use forceps]. I) take 2 absorbent paper and sock with solution A. Ii) take 1 absorbent paper and sock with solution B. 11 Ta...

3

Lab Recepies: Isolation of DNA from Environmental Surface Water Samples

http://labrecepies.blogspot.com/2008/07/isolation-of-dna-from-environmental.html

Isolation of DNA from Environmental Surface Water Samples. Isolation of DNA from Environmental Surface Water Samples. 1 Before starting the experiment take out solution I from the freezer and prepare solution II and collect ice. 2 By shaking the water container take 1.5 ml (750 ml 750 ml) water into eppendorf tube. 3 Centrifuge at 14, 000 rpm for 5-6 minutes. 4 Decant supernatant (SNT) and dry on tissue paper. 5 Add 100 ml Solution I in each tube and mix by pipetting. 10 Collect SNT with cut tips. 21 Inc...

4

Lab Recepies: Isolation of Shigella from environmental surface water samples

http://labrecepies.blogspot.com/2008/07/isolation-of-shigella-from.html

Isolation of Shigella from environmental surface water samples. Isolation of Shigella from environmental surface water samples. 1 At first approximately 100 ml water pass through the 0.45 mm milipore filter. 2 Then the filter was chopped by sterile applicator and incubated in the peptone water (pH 8.0) for 2-4 hrs at 37oC shaker incubator for enrichment. 4 After overnight incubation the suspected Shigella colonies were selected and subjected to serological and biochemical tests for confirmation. Isolatio...

5

Lab Recepies: Some Basic Conversions and Calculations

http://labrecepies.blogspot.com/2008/07/some-basic-conversions-and-calculations.html

Some Basic Conversions and Calculations. 1 gram (g) = 1000 milligrams (mg). 1 milligram (mg) = 1000 microgram (ug). 1 microgram (ug) = 1000 nanogram (ng). 1 nanogram (ng) = 1000 picogram (pg). 1 gram = 10e12 picograms. 1 mole = 1000 millimoles (mmole). 1 millimole (mmole ) = 1000 micromoles (umole). 1 micromole (umole ) = 1000 nanomoles (nmole). 1 nanomole (nmole ) = 1000 picomoles (pmole). 1 mole = 10e12 picomoles. A 1 micromolar solution (1uM) = 1 picomoles per microliter (1pmoles/ul):. 15 mM x 10 ml.

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Lab Recepies

Isolation of DNA from Environmental Surface Water Samples. Isolation of DNA from Environmental Surface Water Samples. 1 Before starting the experiment take out solution I from the freezer and prepare solution II and collect ice. 2 By shaking the water container take 1.5 ml (750 ml 750 ml) water into eppendorf tube. 3 Centrifuge at 14, 000 rpm for 5-6 minutes. 4 Decant supernatant (SNT) and dry on tissue paper. 5 Add 100 ml Solution I in each tube and mix by pipetting. 10 Collect SNT with cut tips. 21 Inc...

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