londonstructuralbiology.org
ISMB-Biophysics Centre*
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ISMB-Biophysics Centre | londonstructuralbiology.org Reviews
https://londonstructuralbiology.org
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londonstructuralbiology.org
ISMB-Biophysics Centre
http://www.londonstructuralbiology.org/overview.html
ISMB research programmes Structural Biology. The ISMB Biophysics Centre. First questions to answer about a newly purified macromolecule are often whether it is monomeric and whether it is folded. AUC. Is the method of choice to determine the oligomeric state, and circular dichroism. Can give a quick, or a detailed answer to questions of fold. For initial tests of enzyme activity and turnover reactions there is a UV/Vis spectrometer. The affinity of a complex is another question AUC. Is intrinsic to many ...
ISMB-Biophysics Centre
http://www.londonstructuralbiology.org/kinetics.html
ISMB research programmes Structural Biology. The ISMB Biophysics Centre. KinetAsysT, Hi-Tech Scientific). Various size syringes are available to allow mixing at different ratios. Double-mixing of samples is also possible to further investigate reactions of more than one step. The syringes and optical cell are immersed in a thermostatted waterbath allowing choice of reaction temperature. For initial enzyme characterisation classical turnover experiments are very important. These give direct informatio...
ISMB-Biophysics Centre
http://www.londonstructuralbiology.org/fluorimetry.html
ISMB research programmes Structural Biology. The ISMB Biophysics Centre. Our FluoroMax3 has a stirred and temperature-controlled 4-position sample changer. Alternatively, a fibre-optic 96/384 microwell-plate reader can be connected for high-throughput applications, e.g. screening of potential ligands or rapid collection of concentration-dependent data. ISMB Biophysics Centre, Birkbeck, University of London.
ISMB-Biophysics Centre
http://www.londonstructuralbiology.org/calorimetry.html
Institute of Structural and Molecular Biology Home. The ISMB Biophysics Centre. A wide range of buffer conditions are suitable. A further advantage is that molecules do not need to be labelled or altered, so the method lends itself to investigations at native conditions. However, a relatively large sample volume is required, so alternatively Surface Plasmon Resonance. Could be used, or, if one of the binding partners is fluorescent, anisotropy measurements. Also for DSC, the solution conditions can be re...
ISMB-Biophysics Centre
http://www.londonstructuralbiology.org/safety.html
ISMB research programmes Structural Biology. The ISMB Biophysics Centre. Please read our COSHH form. And inform us, if your project requires more stringent safety measures. Before commencing work in the ISMB Biophysics Centre you will have to sign it. Please contact. Us if you have any questions. Please also read the safety documents below:. Laboratory Code of Practice. School of Crystallography Safety Page. ISMB Biophysics Centre, Birkbeck, University of London.
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ISMB research programmes Structural Biology. The ISMB Biophysics Centre. The ISMB Biophysics Centre provides access to instrumentation and expertise in the analysis of biomolecular interactions for scientists in London. A variety of instrumentation is available for the characterisation of equilibrium and kinetic behaviours of protein, nucleic acid and small-molecule interactions on a hire, service or collaborative basis. For an overview of the methods and techniques available, please click here. School o...
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