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PNA CRISPR/Cas9 Service | PNA BIOpeptide nucleic acids
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peptide nucleic acids
http://m.pnabio.com/
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PNA CRISPR/Cas9 Service | PNA BIO | m.pnabio.com Reviews
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peptide nucleic acids
PNA design Tool
http://m.pnabio.com/support/PNA_Tool.htm
TEL 1-844-846-0454 (Toll Free) / 805-504-2220 FAX 805-277-0629. Please note that you agree " Terms of Use. When you purchase our product.
Reporter system for CRISPR/Cas9 enrichment and activity validation | PNA BIO
http://m.pnabio.com/products/Reporter.htm
Targeting efficiency of Engineered nucleases ranges 1% 30% for the most cases. Although this efficiency is quite remarkable compared to conventional gene targeting techniques by homologous recombination, steps to isolate cells bearing desired mutations still require extensive screening. Our knockout cell enrichment system has been developed to overcome such limitations. The use of surrogate reporters allows selective enrichment of cells that have acquired EN mediated mutations at target sites. Empty surr...
Cas9 protein with NLS & CRISPR/Cas9 service | PNA BIO
http://m.pnabio.com/products/CRISPR_Cas9.htm
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), mostly found in bacteria, are loci containing short direct repeats of 24 48. CRISPR is a form of acquired prokaryotic immune system, conferring resistance to exogenous sequcences such as plasmids and phages. RGENs (RNA Guided Endonucleases). RGENs recognize a target specific sequence that is 23bp in length, ending with two guanines (GG). This minimal requirement for target site selection means that RGENs can be designed to cleave any...
PNA FISH probes | PNA Bio Inc
http://m.pnabio.com/products/PNA_FISH.htm
PNA probes hybridize to target DNA with high sensitivity and specificity mainly due to electrical neutrality of its chemical structure. This trait enables PNA probes to be more efficient and useful in applying to FISH (fluorescent in situ hybridization) even at low concentrations. Hybridization occurs fast (within a couple of hours) and background noise is reduced significantly. Cent probe is specific for human alpha satellite centromere and does not react to mouse. F1002 and F3001 (CENPB-FAM). Most FISH...
KO/KI cell line service using CRISPR/Cas9
http://m.pnabio.com/products/KOCells.htm
Process of generating knock-out and knock-in cells. PNA Bio offers economic cell line generation (knock-outs and knock-ins) in a timely manner. The process of engineered cell line generation is shown below. Design and synthesis of 4 8 gRNA vectors. Select the most efficient engineered nuclease by mismatch sensitive nuclease assay. Synthesis of reporter vector for enrichment of cells with gene editing event. Optimization of transfection protocol. Design of donor vector for knock-in project. We offer the m...
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PNA CRISPR/Cas9 Service | PNA BIO
TEL 1-800-764-0478 (Toll Free) / 805-504-2220 FAX 805-277-0629.
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