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MCF-7 Cells (human breast adenocarcinoma cell line) Cell Culture Protocol and Transfection Resources

MCF-7 cells (human breast adenocarcinoma cell line) cell line information. cell culture protocol

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MCF-7 Cells (human breast adenocarcinoma cell line) Cell Culture Protocol and Transfection Resources | mcf7.com Reviews
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MCF-7 Cells (human breast adenocarcinoma cell line) Cell Culture Protocol and Transfection Resources | mcf7.com Reviews

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MCF-7 Cell Culture Protocol Transfection | MCF-7 Cells

http://www.mcf7.com/cell-culture

Human breast adenocarcinoma cell line. Use Eagle’s MEM, supplemented with 10% FBS, 1% penicillin/streptomycin. Can also add non essential amino acids (0.1 mM), Insulin (10ug/mL) and Sodium pyruvate (1mM). Add 10nM estrogen to media for a 3-4x increase in cell numbers. Maintain temperature at 37 C in humidified, concentrated CO2 (5%) atmosphere. Once MCF-7 cells reach approximately 90% confluence on plates, remove media and passage cells by rinsing with 1xPBS twice. Purchase MCF-7 Transfection Reagent.

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Your first blog post. You are browsing the archive for Uncategorized. Larr; Previous Entries. Can we edit GABRA3 to suppress AKT activation and therefore metastasis in breast cancer. March 18, 2016 in Uncategorized. Women and men are diagnosed with breast cancer each year in the UK and out of this 11,500 dying from this disease. With the rate of breast cancer increasing by 3.5%. A year it’s never been more important to find a way of reducing these statistics. Why is Gabra3 important? Cells expressing Gab...

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MCF-7 Cells (human breast adenocarcinoma cell line) Cell Culture Protocol and Transfection Resources

Human breast adenocarcinoma cell line. Origin of MCF-7 Cell Line. MCF-7, a widely studied epithelial cancer cell line derived from breast adenocarcinoma, has characteristics of differentiated mammary epithelium. MCF7 cells can be used for detecting PI3K and MAPK involvment, along with easy detection of ERK and Akt phsophorylation. Also, via cytoplasmic estrogen receptors, these cells have the ability to process estradiol. Uses for MCF-7 Cell Line. MCF-7 cells are useful for. MCF-7 cells are fairly large ...

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