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Microbe Pundit: October 2006
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Tuesday, October 31, 2006. Q and A on BACs and Metagenomics. Why large inserts? Question from student (edited):. Yes, you are correct, and yes, there is another reason. Large inserts mean that each clone ( Escherichia coli. Posted by David Tribe at 12:51 pm. Links to this post. A question of cycles of oxidation in mineral leaching. Question from student (edited):. Does the H2SO4 that is produced cause the leaching out of other metals from their ores because of its acidic nature? Links to this post. MUTAN...
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Microbe Pundit: March 2007
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Thursday, March 22, 2007. Welcome to Microbe Pundit , all students of biomolecular engineering! Study questions on the fueling reactions of biosynthesis. What is the justification for combining energy and reducing power into a single entity-driving force? ATP generation by transmembrane ion gradients involves two steps: establishing the gradient, and using its energy to produce ATP. What mechanisms have microbes evolved for these two steps? Respond in the comments and get feedback from The Pundit.
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Microbe Pundit: September 2006
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Tuesday, September 05, 2006. All you need to now about scale-up and scale down, overflow metabolism and glucose feeding when using Escherichia coli as a process tool. Figure 1. Experimental set-up for scale-down simulation studies. (a). Large-scale STR. Va is the addition zone where the most extreme concentration. Gradients are known to exist and Vb is the bulk region that can be. Considered to be well mixed. (b) Scale-down simulation equipment. Here. Times are high (greater than 50 seconds even at the 2...
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Microbe Pundit: July 2006
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Friday, July 28, 2006. Assays for compounds that make existing antibiotics effective against antibiotic resistant bacteria. The figure above shows structures of the beta-lactam semi-synthetic antibiotic amoxycillin. Top) and the different compound clavulanate. The useful beta lactamase inhibitor clavulanate was discovered around 1970 using a clever but simple assay strategy. It required a simple way of measuring inhibition of beta lactamase enzyme activity. This is because it changes from yellow. The mos...
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Microbe Pundit: July 2007
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Friday, July 27, 2007. Rich pickings for proposals in dairy microbe biotechnology. This is what a quick literature search with PubMed can reveal:. J Bacteriol. 2007 Apr;189(8):3256-70. Epub 2007 Feb 16. Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis. Wegmann U, O'Connell-Motherway M, Zomer A, Buist G, Shearman C, Canchaya C, Ventura M, Goesmann A, Gasson MJ, Kuipers OP, van Sinderen D, Kok J. PMID 17307855 [PubMed - indexed for MEDLINE]. PMID 9767571 [PubMed - indexed ...
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Microbe Pundit: March 2006
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Monday, March 13, 2006. Replication mechanisms of the bacterial chromosome. A timeless experiment in cell biology by an Australian scientist. FIGURE 5-2 from here. Autoradiograph of intact replicating chromosome of. Schaecter et al. Microbe Chapters 8, 10 are very useful reading on this topic. There is also the Baron free online textboo. The major points made in the teaching session were:. The structure of a DNA molecule including the backbone and pairing of bases. How DNA and RNA differ. A description o...
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Microbe Pundit: August 2006
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Tuesday, August 29, 2006. Entry of dyes can be sensitive to the state of the membrane. Schematic diagram of a living (bacterial) cell with its polarised (charged) membrane. The cytoplamic membrane is represented in red. PI is a cationic dye (propidium iodide) that binds to DNA, and it is not lipid soluble. BOX is lipophilic oxanol dye, negatively ionised, and about 500 MW. Both PI and BOX are fluorescent. Question: What are the factors affecting staining of the cell by these dyes? Links to this post.
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Microbe Pundit: June 2007
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Saturday, June 16, 2007. Energy and gold among the SliMEs. Clean fuel research deal agreed by BP and Synthetic Genomics. Synthetic Genomics Inc., a privately-held company dedicated to commercializing synthetic genomic processes and naturally occurring processes for alternative energy solutions, has announced a significant, long-term research and development deal with BP. The overall goal of Synthetic Genomics is to discover and/or design new genomes that will code for new types of cells with desired prop...
