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Tuesday, October 31, 2006. Problems with the riboswitch assay. This week I got the preliminary riboswitch experiment results. I tested sxy RNA for binding ATP, GTP, dATP. dGTP, guanosine, adenosine, AMP and GMP (separately). Each of these substances was incubated in three different concentrations (10 nM, 300nM and 100uM) with labeled sxy RNA in a special buffer that contains Mg and K. I also had three controls:. 1) RNA incubated with 100uM of ligand and not digested with RNase (negative control). CHAPTER...

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Tuesday, October 31, 2006. Problems with the riboswitch assay. This week I got the preliminary riboswitch experiment results. I tested sxy RNA for binding ATP, GTP, dATP. dGTP, guanosine, adenosine, AMP and GMP (separately). Each of these substances was incubated in three different concentrations (10 nM, 300nM and 100uM) with labeled sxy RNA in a special buffer that contains Mg and K. I also had three controls:. 1) RNA incubated with 100uM of ligand and not digested with RNase (negative control). CHAPTER...

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mvresearch | mvresearch.blogspot.com Reviews

https://mvresearch.blogspot.com

Tuesday, October 31, 2006. Problems with the riboswitch assay. This week I got the preliminary riboswitch experiment results. I tested sxy RNA for binding ATP, GTP, dATP. dGTP, guanosine, adenosine, AMP and GMP (separately). Each of these substances was incubated in three different concentrations (10 nM, 300nM and 100uM) with labeled sxy RNA in a special buffer that contains Mg and K. I also had three controls:. 1) RNA incubated with 100uM of ligand and not digested with RNase (negative control). CHAPTER...

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mvresearch.blogspot.com

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mvresearch: Always look on the bright side of life..

http://mvresearch.blogspot.com/2006/10/always-look-on-bright-side-of-life.html

Tuesday, October 03, 2006. Always look on the bright side of life. Ok, I am closer to the beginning of the end with my thesis writing. I admit that it makes me really happy the fact that I have to use an industrial stapler to staple my thesis together. Also the Rosie's comments are getting much shorter and there is more "good"s on the sides of my poor paragraphs. I think that these are all the signs that I am getting there. End now for something completely different-. Does anyone have an explanation?

2

mvresearch: still working on the gel picture........

http://mvresearch.blogspot.com/2006/09/still-working-on-gel-picture.html

Tuesday, September 12, 2006. Still working on the gel picture. I ran some gels last week and it turned out that the label (on the RNA) was really old so I had to expose them for days and days to be able to visualize the bands (and they were still fuzzy). I ran another gel today and I will have to leave for at least 4 days to get something (I am getting really annoyed with it). Posted by Okapia Johnstoni @ 6:45 PM. I wouldn't mind learning some of the history of your work. Good luck with your thesis!

3

mvresearch: Problems with the riboswitch assay

http://mvresearch.blogspot.com/2006/10/problems-with-riboswitch-assay.html

Tuesday, October 31, 2006. Problems with the riboswitch assay. This week I got the preliminary riboswitch experiment results. I tested sxy RNA for binding ATP, GTP, dATP. dGTP, guanosine, adenosine, AMP and GMP (separately). Each of these substances was incubated in three different concentrations (10 nM, 300nM and 100uM) with labeled sxy RNA in a special buffer that contains Mg and K. I also had three controls:. 1) RNA incubated with 100uM of ligand and not digested with RNase (negative control).

4

mvresearch: October 2006

http://mvresearch.blogspot.com/2006_10_01_archive.html

Tuesday, October 31, 2006. Problems with the riboswitch assay. This week I got the preliminary riboswitch experiment results. I tested sxy RNA for binding ATP, GTP, dATP. dGTP, guanosine, adenosine, AMP and GMP (separately). Each of these substances was incubated in three different concentrations (10 nM, 300nM and 100uM) with labeled sxy RNA in a special buffer that contains Mg and K. I also had three controls:. 1) RNA incubated with 100uM of ligand and not digested with RNase (negative control). CHAPTER...

5

mvresearch: September 2006

http://mvresearch.blogspot.com/2006_09_01_archive.html

Tuesday, September 26, 2006. Very first thesis draft. Last week I have been working on writing of the discussion section and preparation of my first thesis draft. The discussion section is still not fully developed but I got a very basic first draft. Posted by Okapia Johnstoni @ 9:44 AM 0 comments. Monday, September 18, 2006. Discussion on my thesis discussion. They say it comes with time and practice, but I do not have either. Today I started working on the discussion section. I made a very rough ou...

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Tuesday, October 31, 2006. Problems with the riboswitch assay. This week I got the preliminary riboswitch experiment results. I tested sxy RNA for binding ATP, GTP, dATP. dGTP, guanosine, adenosine, AMP and GMP (separately). Each of these substances was incubated in three different concentrations (10 nM, 300nM and 100uM) with labeled sxy RNA in a special buffer that contains Mg and K. I also had three controls:. 1) RNA incubated with 100uM of ligand and not digested with RNase (negative control). CHAPTER...

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