ncxt.lbl.gov

National Center for X-ray Tomography

The National Center for X-ray Tomography develops novel imaging technologies for biological and biomedical research. In particular, NCXT staff and collaborators are spearheading the development of soft x-ray tomography. As a new tool for visualizing cells. A major part of this development has been the design, construction and now operation of XM-2. The worlds first soft x-ray microscope for life science research. Located at the Advanced Light Source. Of Lawrence Berkeley National Laboratory. This techniq...

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National Center for X-ray Tomography | ncxt.lbl.gov Reviews

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The National Center for X-ray Tomography develops novel imaging technologies for biological and biomedical research. In particular, NCXT staff and collaborators are spearheading the development of soft x-ray tomography. As a new tool for visualizing cells. A major part of this development has been the design, construction and now operation of XM-2. The worlds first soft x-ray microscope for life science research. Located at the Advanced Light Source. Of Lawrence Berkeley National Laboratory. This techniq...

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4 tutorials

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10 supported by

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National Center for X-ray Tomography | ncxt.lbl.gov Reviews

https://ncxt.lbl.gov

The National Center for X-ray Tomography develops novel imaging technologies for biological and biomedical research. In particular, NCXT staff and collaborators are spearheading the development of soft x-ray tomography. As a new tool for visualizing cells. A major part of this development has been the design, construction and now operation of XM-2. The worlds first soft x-ray microscope for life science research. Located at the Advanced Light Source. Of Lawrence Berkeley National Laboratory. This techniq...

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ncxt.lbl.gov

1

Tutorials | National Center for X-ray Tomography

http://ncxt.lbl.gov/tutorials

Below, you can find links to tutorials covering all aspects of the imaging carried out at the NCXT. If you notice an error, or have suggestions how to improve the clarity or increase the scope of these tutorials, please contact us. We are also building a library of in-depth instructional videos. Covering all aspects of soft x-ray tomography, from making capillaries and preparing specimens to setting up the microscope and collecting data. Preparing Specimen Mounting Capillaries. BD FACSaria Cell Sorter.

2

Soft X-ray Tomography | National Center for X-ray Tomography

http://ncxt.lbl.gov/sxt

Our understanding of cell structure and function depends heavily on imaging techniques. You can read about XM-2 below, or download this paper as a pdf. In addition to data acquisition, we are collaborating with Dr. Chao Yang to develop AREC3D, a software package for automated data processing and reconstruction. Useful links related to soft x-ray tomography:. The Advanced Light Source (ALS website). Tutorial on synchrotron radiation (from the ALS). BD FACSaria Cell Sorter.

3

Questions? Comments? Got an idea for a collaboration? | National Center for X-ray Tomography

http://ncxt.lbl.gov/node/1301

Got an idea for a collaboration? BD FACSaria Cell Sorter. Imaging and characterizing cells using tomography. Analysis of ER-mitochondria contacts using correlative fluorescence microscopy and soft X-ray tomography of mammalian cells. Progressive Chromatin Condensation and H3K9 Methylation Regulate the Differentiation of Embryonic and Hematopoietic Stem Cells.

4

Operating Manual | National Center for X-ray Tomography

http://ncxt.lbl.gov/manual

5

Zeiss Axiovert 200M Fluorescence/Live cell Imaging Microscope | National Center for X-ray Tomography

http://ncxt.lbl.gov/node/705

Zeiss Axiovert 200M Fluorescence/Live cell Imaging Microscope. This is the state-of-the-art wide-field microscope equipped with a temperature controlled CO2-incubation system for live cell time-lapse experiments. Zeiss Axiovert 200 M inverted microscope with fluorescence / phase or DIC (Nomarski) imaging. Apotome slider module for high quality optical section. Motorized filter wheel with Dapi, CFP, GFP, Texas Red and bright field. Other filter sets availables are: Cy5 and QDot 605. BD FACSaria Cell Sorter.

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Structural Biology: User Facilities Enabling Science

http://www.doegenomestolife.org/userfacilities/structuralbio.shtml

About Genomic Science Program. DOE Office of Science Statement on Digitial Data Management. USDA/DOE Feedstock Genomics Program. Carbon Cycling, Biogeochemical Processes. DOE National Laboratory Science Focus Areas (SFAs). About the User Facilities. DOE Joint Genome Institute (JGI). DOE Environmental Molecular Sciences Laboratory (EMSL). Structural Biology Resources (SBR). Office of Biological and Environmental Research. US Department of Energy Office of Science. User Facilities Enabling Science. Synchro...

genomicscience.energy.gov

Structural Biology: User Facilities Enabling Science

http://genomicscience.energy.gov/userfacilities/structuralbio.shtml

About Genomic Science Program. DOE Office of Science Statement on Digitial Data Management. USDA/DOE Feedstock Genomics Program. Carbon Cycling, Biogeochemical Processes. DOE National Laboratory Science Focus Areas (SFAs). About the User Facilities. DOE Joint Genome Institute (JGI). DOE Environmental Molecular Sciences Laboratory (EMSL). Structural Biology Resources (SBR). Office of Biological and Environmental Research. US Department of Energy Office of Science. User Facilities Enabling Science. Synchro...

genomicsgtl.energy.gov

Structural Biology: User Facilities Enabling Science

http://genomicsgtl.energy.gov/userfacilities/structuralbio.shtml

About Genomic Science Program. DOE Office of Science Statement on Digitial Data Management. USDA/DOE Feedstock Genomics Program. Carbon Cycling, Biogeochemical Processes. DOE National Laboratory Science Focus Areas (SFAs). About the User Facilities. DOE Joint Genome Institute (JGI). DOE Environmental Molecular Sciences Laboratory (EMSL). Structural Biology Resources (SBR). Office of Biological and Environmental Research. US Department of Energy Office of Science. User Facilities Enabling Science. Synchro...

www-als.lbl.gov

ALS Beamlines Directory

http://www-als.lbl.gov/index.php/beamlines/beamlines-directory.html

Report and Search for Publications. Access to the ALS. In Case of Emergency. Beamlines, Parameters, Contact Information, and Schedules. Click on the image to see/download a high-resolution version of the ALS beamclock. Beamline and endstation technical information is available through the links below. Unless otherwise noted, all beamlines are currently operational. Individual beamline schedules are posted when available. National Center for X-Ray Tomography (NCXT). 400 - 1300 eV. This e-mail address is b...

anatomy.ucsf.edu

Faculty

http://anatomy.ucsf.edu/faculty

University of California San Francisco. PhD Assistant Professor lab website. PhD Professor and Chair lab website. Nripendra Dhillon, MBBS, MS Lecturer. Annemarie Donjacour, PhD Assistant Adjunct Professor. Amber Fitzsimmons, PhD Assistant Adjunct Professor. Derek Harmon, PhD Assistant Adjunct Professor. PhD Professor and Vice Chair lab website. PhD Adjunct Professor lab website. MD, PhD Professor lab website. MD, PhD Professor lab website. PhD Assistant Professor lab website. MD, PhD Professor lab website.

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ncxt.lbl.gov

National Center for X-ray Tomography

The National Center for X-ray Tomography develops novel imaging technologies for biological and biomedical research. In particular, NCXT staff and collaborators are spearheading the development of soft x-ray tomography. As a new tool for visualizing cells. A major part of this development has been the design, construction and now operation of XM-2. The worlds first soft x-ray microscope for life science research. Located at the Advanced Light Source. Of Lawrence Berkeley National Laboratory. This techniq...

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